Vitamin K2 high-yielding strain

A technology for high-yielding strains and vitamins, applied in bacteria, microorganisms, microorganism-based methods, etc., can solve the problems of reduced affinity between prothrombin and calcium ions, and obstruction of the coagulation process.

Inactive Publication Date: 2012-12-19
上海红马饲料有限公司
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Problems solved by technology

Studies have shown that vitamin K 2 A variety of proteins involved in the synthesis of blood coagulation, in the process of formation, prothrombin and phospholipids form a polymer, in which calcium ions

Method used

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Embodiment Construction

[0021] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific illustrations.

[0022] Such as figure 1 Shown, vitamin K of the present invention 2 The high-yield strain is obtained by screening the mutant BS-53 protoplast of bacillus natto BS-5 and the protoplast of bacillus natto CICC10262 after fusion.

[0023] In the present invention, the mutant BS-53 is obtained by compound mutagenesis of Bacillus natto BS-5 through ultraviolet and nitroso arc.

[0024] The Bacillus natto BS-5 strain is provided by the Microbiology Laboratory of the School of Food Science and Technology, Hefei University of Technology, and the Bacillus natto CICC10262 is provided by the China Microorganism Culture Collection and Management Center.

[0025] The Bacillus natto BS-5 bacterial strain is a kind of Bacillus subtilis Gram that can produce ...

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Abstract

The invention aims to disclose a vitamin K2 high-yielding strain. Compared with the prior art, bacillus natto BS-5 is taken as an original strain, after the ultraviolet and nitroso arc composite mutation, the mutant strain BS-53 is obtained after screening, the mutant strain BS-53 and bacillus natto CICC (China center of industrial culture collection)10262 which has low vitamin K2 yield but high growth rate, are subjected to protoplast fusion, fusant is obtained through the screening of a flat screen, and the fusant is finally obtained after the selecting of the fermenting property experiment; and the vitamin K2 high-yielding strain has excellent characteristics of parents, i.e. has the dual effects of high MK (midkine) yield and high growth rate, an effective method is provided for realizing high MK yield, important scientific meaning and application value are obtained, and the purpose of the invention is realized.

Description

technical field [0001] The present invention relates to a bacterial strain, in particular to a vitamin K 2 High-yielding strain. Background technique [0002] Vitamin K is a class of naphthoquinone derivatives with biological activity of phylloquinone, which plays an important role in controlling blood coagulation. naturally occurring vitamin K 1 and K 2 , Vitamin K 1 Widely present in green plants, it is an important source of vitamin K in food; vitamin K 2 It is mainly synthesized by the intestinal bacteria of the human body. [0003] Studies have found that vitamin K 2 Belonging to fat-soluble vitamins, it has the functions of maintaining the normal blood coagulation of the body, preventing osteoporosis and anti-cancer. Studies have shown that vitamin K 2 A variety of proteins involved in the synthesis of blood coagulation, in the process of formation, prothrombin and phospholipids form a polymer, in which calcium ions can promote the synthesis of polymers, but in...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/03C12R1/07
Inventor 刘国庆赵肖胡卫华钱晓勇
Owner 上海红马饲料有限公司
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