Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and device for hollow focused light spot excitation-based confocal microscopy

A technology of confocal microscopy and focusing spot, which is applied in the field of microscopy, can solve problems such as limiting the effective point spread function, and achieve the effects of simple structure, small system changes, and high system limit resolution

Inactive Publication Date: 2015-03-18
ZHEJIANG UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Compared with the traditional wide-field microscope system, the confocal microscope system adopts scanning imaging, and places a pinhole (Pinhole) on the focal plane conjugate to the imaging object plane to block the stray light around the non-imaging point , which effectively limits the effective point spread function of the system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for hollow focused light spot excitation-based confocal microscopy
  • Method and device for hollow focused light spot excitation-based confocal microscopy
  • Method and device for hollow focused light spot excitation-based confocal microscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0052] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, but the present invention is not limited thereto.

[0053] Such as figure 1 Shown is the confocal microscopy device based on hollow focusing spot excitation of the present invention, comprising:

[0054] Laser 1, phase encoder 2, polarization state converter 3, microscope objective lens 4, dichroic plate 5, convex lens 6, pinhole 7, photoelectric sensor 8, computer 9, two-dimensional mobile platform 10, fluorescent sample 11, two Dimensional scanning galvanometer 12.

[0055] The laser light emitted by the laser 1 becomes the incident beam of the system after linear polarization, beam collimation and monochromatic filtering inside the laser 1 , and the central wavelength of the beam is determined by the fluorescence absorption spectrum of the fluorescent sample 11 . The incident beam will be vortex phase encoded after passing through the phase encoder...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
refractive indexaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for hollow focused light spot excitation-based confocal microscopy. The method comprises the following steps of 1, transforming laser beams into circularly polarized lights by phase encoding, 2, by a microscope objective, focusing the circularly polarized lights to a sample needing to be detected so that a hollow focused light spot is formed and fluorescence is excited, and 3, scanning the surface of the sample needing to be detected, collecting the excited fluorescence, acquiring light intensity information at different positions and carrying out calculation to obtain a corresponding microscopic image. The invention also discloses a device for hollow focused light spot excitation-based confocal microscopy. The method and the device realize a higher system limit resolution ratio and have a small system change, a simple structure and an imaging rate the same as an imaging rate of the original confocal system.

Description

technical field [0001] The invention relates to the field of microscopes, in particular to a confocal microscopy method and device based on excitation of a hollow focal spot. Background technique [0002] Like most optical imaging, the Abbe diffraction limit has restricted the improvement of the resolution of the microscope system since the invention of the microscope. Early microscope systems were all wide-field imaging systems with limited imaging resolution. This situation was not improved to some extent until the invention of the confocal microscope system (Confocal Microscope). [0003] The basic concept of confocal microscopy was proposed by M.Minsky et al. in 1957 [M.Minsky et al. Microscopy Apparatus, US Patent 3013467], but the technology was not truly instrumented until 1978 [C.Cremer et al. Considerations on a laser -scanning-microscope with high resolution and depth of field]. [0004] Laser confocal scanning microscope uses laser light as scanning light sourc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 匡翠方郝翔李旸晖顾兆泰李帅刘旭
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com