The invention relates to a chip-level bottom filling adhesive, which comprises following components by weight percentage: 15 to 50 percent of epoxy resin, 1 to 20 percent of toughening agent, 0.1 to 1 percent of dispersing agent, 0.01 to 1 percent of defoaming agent, 0.8 to 10 percent of cross-linking agent, 0.1 to 0.5 percent of pigment, 40 to 70 percent of filler, 3 to 30 percent of curing agent and 1 to 20 percent of diluting agent. A preparation method of the chip-level bottom filling adhesive comprises the steps that the epoxy resin, toughened resin, the dispersing agent, the defoaming agent, the cross-linking agent and the pigment are weighed according to the proportion, thrown into reaction kettle and mixed to form a homogeneous solution; and afterwards, the filler, the curing agent and the diluting agent are weighed according to the proportion, thrown into the reaction kettle in sequence and evenly mixed with the homogeneous solution, and then the chip-level bottom filling adhesive is obtained. The bottom filling adhesive prepared by adopting the method has the characteristics of low curing shrinkage rate, high reliability, and the like, simultaneously satisfies the requirement of low radioactivity and is suitable for primary high-density packaging of memory chips.