Functional strain, and microbial agent and application thereof

A strain and functional technology, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problems of general deodorization effect, human injury, and olfactory failure, and achieve significant deodorization and antibacterial effects

Inactive Publication Date: 2013-01-02
HAINAN FANGLVYUAN TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term repeated stimulation by foul-smelling substances can also cause olfactory fatigue and lead to loss of smell
[0004] The main deodorizing methods currently used are: 1. Sensory deodorization: using aromatic organic compounds, masking agents, animal and plant essential oils, etc. to deodorize by covering and covering, such as air fresheners, which cover odors by emitting aromas , does not decompose and remove harmful gases in the air, but "dilutes" the peculiar smell by confusing people's sense of smell, and the ingredients it contains may cause harm to the human body; 2. Physical deodorization: use absorption and adsorption to make The odorous gas in the air reacts with water or other chemical substances to form odorless molecules, or use porous media to adsorb odorous molecules to achieve deodorization effects, such as using water washing, organic solvent dissolution, etc., or using active The adsorption of carbon, zeolite, activated clay, etc. is only suitable for the treatment of low-concentration malodorous gases, and the deodorization effect is general, and the investment is high; The microbial film on the surface oxidizes the waste odor molecules and decomposes them into odorless simple inorganic substances to achieve the purpose of deodorization. It is currently the most economical and effective deodorization method. It can simultaneously treat waste gas containing multiple pollutants and is environmentally friendly. Hygienic, no secondary pollution, but it requires that the odorous substances to be removed have good water solubility and can be oxidized by microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment one, the preparation of microbial bacterial agent:

[0027] 1. Strain activation: Inoculate the Bacillus subtilis strain W58 stored at -20°C on LB plate medium, and culture at 30°C for 22 hours to obtain activated functional strains;

[0028] 2. Preparation of seed liquid: Pick a single colony and insert the activated functional strain into LB liquid medium, shake and culture at 28°C for 24 hours to obtain seed liquid;

[0029] 3. Fermentation culture: the seed solution obtained in step (2) is inserted into the fermentation culture medium according to the volume percentage of 4%, and then cultivated for 26 hours at 30°C and a shaker speed of 170rpm, to obtain the active ingredient of subtilis subtilis Liquid microbial inoculant of Bacillus strain W58. The composition of the fermentation medium is: K 2 HPO 4 0.6%, KH 2 PO 4 0.1%, NaCl0.3%, MgSO 4 ·7H 2 O0.02%, CaCl 2 2H 2 O0.025%, (NH 4 ) 2 SO4 0.2%, 4% sucrose, 3% soybean powder, and the balance i...

Embodiment 2

[0030] Embodiment two, the preparation of microbial bacterial agent:

[0031] 1. Strain activation: Inoculate the Bacillus subtilis strain W58 stored at -20°C on LB plate medium, and culture it at 28°C for 24 hours to obtain activated functional strains;

[0032] 2. Preparation of seed solution: pick a single colony and insert activated functional strain into LB liquid medium, shake and culture at 32°C for 20 hours to obtain seed solution;

[0033] 3. Fermentation culture: the seed solution obtained in step (2) is inserted into the fermentation culture medium according to the volume percentage of 8%, and then cultivated for 28 hours at 28°C and a shaker speed of 190rpm, and the active ingredient is Subtilis subtilis Liquid microbial inoculant of Bacillus strain W58. The composition of the fermentation medium is: K 2 HPO 4 0.4%, KH 2 PO 4 0.3%, NaCl0.2%, MgSO 4 ·7H 2 O0.04%, CaCl 2 2H 2 O0.020%, (NH 4 ) 2 SO 4 0.3%, 3% sucrose, 1% soybean powder, and the balance is ...

Embodiment 3

[0034] Embodiment three: test

[0035] To a pile of rubbish that produces stench, the liquid microbial bacterial agent (diluted 10 times with tap water before use) of embodiment one gained is sprayed with sprayer, make preparation spraying range throughout the rubbish dumping area as far as possible. Sampling was carried out at different locations around the garbage, and H was measured 20 minutes before and after spraying the preparation. 2 S and NH 3 Concentration, the sampling point is 1m away from the edge of the odor source, the sampling height is 1m, 5 points are sampled, and the average value is taken. Odor concentration adopts three-point comparative smelly bag method (GB / T14675); ammonia adopts sodium hypochlorite-salicylic acid spectrophotometer (GB / T 14679); hydrogen sulfide adopts polyvinyl alcohol ammonium phosphate absorption-methylene blue colorimetric method (GB / T 16489). The results obtained are shown in Table 2.

[0036] Table 2

[0037] Test it...

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PUM

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Abstract

The invention belongs to the field of environment protection, and specifically relates to a bacillus subtilis strain which is collected in China General Microbiological Culture Collection Center on August 15th, 2011 and has a collection register number of CGMCC No. 5140. The stain is named as W58, and has a categorical name of bacillus subtilis. The stain has substantial deodorizing and antibacterial effects against various effluvial environments. A microbial agent produced by using the bacillus subtilis strain W58 has relatively high NH3 average removal rate, relatively high H2S average removal rate, and relatively high odor concentration average removal rate. The microbial agent can be applied in deodorizing and purifying of garbage, domestic sewage, poultry house air, and the like.

Description

technical field [0001] The invention belongs to the field of environmental protection, and in particular relates to a Bacillus subtilis strain capable of absorbing and degrading malodorous gas substances, and a microbial bacterial agent produced by using the functional strain and its application. Background technique [0002] Stench is a general term for various smells (odors), and is one of seven typical public hazards such as air pollution, water pollution, soil pollution, and noise pollution in the world today. It directly affects people's sense of smell and causes direct harm to people. Among the more than 2 million compounds in nature, more than 4,000 compounds can be recognized by human perception to have foul odors. Except for specific industrial odors, compounds such as hydrogen sulfide, amines, indoles, lower fatty acids, hydrocarbons, and aldehydes It is more harmful to the human body. [0003] Odor pollutants refer to all odor substances that stimulate the olfact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61L9/01C12R1/125A61L101/52
Inventor 王志伟
Owner HAINAN FANGLVYUAN TECH DEV
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