Extraction technology of cerebral polypeptides

An extraction process and brain polypeptide technology, applied in the field of brain polypeptide extraction process, can solve the problems of incomplete virus elimination, long time, unsafe and environmental protection, etc., and achieve the effects of enriching biological activity elements, reducing energy consumption, and shortening the production cycle.

Inactive Publication Date: 2013-01-09
江西康宝医药生物科技有限公司
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Problems solved by technology

[0002] Brain polypeptide is a good biological preparation for brain and brain. Pig brain, because of its low cost and abundant availability, is used to make brain polypeptide series products. The existing brain polypeptide

Method used

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Embodiment Construction

[0006] This specific embodiment adopts the following technical scheme: its technological process is: take the fresh pig brain through quarantine to remove fascia, fat, and brain vertebral body impurities, use purified water to wash, add 4 times the amount of purified water and use a high-speed masher (speed 20,000 r / min) divide it into two high-speed broken cells; filter with a 20-mesh filter cloth, homogenize with a homogenizer (pressure above 200Kg); add citric acid to adjust the pH value, and use compound enzymes to hydrolyze to obtain low Small molecular weight peptides, free amino acids, and nucleotide mixtures, the enzymolysis solution is heated to 40-50°C to deactivate the enzyme, then the deactivation solution is divided into containers, and placed at -20 0 C freezer, take out after freezing for 12-24 hours, thaw at room temperature, take the supernatant and use a centrifuge for high-speed centrifugation to remove fat, cell debris, residual denatured protein and nucleic...

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Abstract

The invention discloses an extraction technology of cerebral polypeptides, relating to the technical field of biochemical industry. The technology comprises the following steps: taking quarantined fresh pig brains and removing impurities, washing with purified water, then adding 4 times of purified water and conducting high speed cell breakage with a high-speed blender at twice; filtering with a filter cloth, and then homogenating by a homogenizer; adjusting the pH value, hydrolyzing by using complex enzyme to obtain low molecular weight small peptide, a mixture of free amino acid and nucleotide, heating up the enzymatic hydrolysate to 40-50 DEG C for removing active enzymes, distributing the enzyme removal liquid into containers, putting the containers in a refrigerator of -200 DEG C, freezing the containers for 12-24h and then taking the containers out, putting the containers at room temperature for thawing, taking the supernatant and centrifuging the supernatant with a centrifugal machine, removing fat, cell debris, residual denatured protein, nucleic acid and other large molecular impurities, taking the centrifugate and conducting unltrafiltration by using an unltrafiltration device. Compared with traditional process, the technology disclosed herein has the advantages of time saving, high efficiency, safety and environmental protection.

Description

technical field [0001] The invention relates to the field of chemical and biological technology, in particular to a brain polypeptide extraction process. Background technique [0002] Brain polypeptide is a good biological preparation for brain and brain. Pig brain, because of its low cost and abundant availability, is used to make brain polypeptide series products. The existing brain polypeptide extraction process adopts conventional repeated freezing and thawing Technology, this technology takes a long time, the virus is not completely eliminated, and the production process is not safe and environmentally friendly. Contents of the invention [0003] The purpose of the present invention is to provide a brain polypeptide extraction process, which strengthens the biosafety control of raw material sources and production processes, adopts nano-scale cell crushing technology, and the nano-scale cell crushing rate is ≥ 95%, which is higher than that of conventional nano-scale c...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 聂建群
Owner 江西康宝医药生物科技有限公司
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