Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method

A fluorescence detection and kinesiology technology, applied in fluorescence/phosphorescence, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of cumbersome operation, difficult interpretation of results, poor repeatability, etc., saving manpower, material resources and time. Effect

Active Publication Date: 2014-11-19
山东百福基因科技有限公司 +4
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  • Claims
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Problems solved by technology

These methods have different defects, such as cumbersome operation, difficult interpr

Method used

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  • Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method
  • Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method
  • Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method

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Embodiment 1

[0029] The kit of the present invention detects the DNA samples of 40 different individuals. The primers used for the detection of motor function-related genes were labeled with blue fluorescent dye, and the internal standard was labeled with red fluorescent dye.

[0030] 1. 40 samples to be tested, the results of 3 individuals with different genotypes are listed below. Relevant samples have been sequenced and tested for ACE using the technical method of "DNA extraction-PCR amplification-sequencing". Among them, the typing results of samples No. 71-2, No. 74-2, and No. 69-4 were: D / D, I / I, I / D.

[0031] 2. Genomic DNA extraction from samples

[0032] Chelex extraction method: Cut off 1-3mm blood spot and put it in a 1.5mL centrifuge tube, add sdH 2 O 1mL, shake and centrifuge, discard the supernatant, repeat the steps twice, discard the supernatant, shake and suspend 5% Chelex-100, quickly draw 200μL into the centrifuge tube with the tip of the cut pipette, and shake for a ...

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Abstract

The invention discloses a fluorescence test kit for a motor-function-related gene ACE (Angiotensin Converting Enzyme) and a detection method, belonging to the technical field of biological detection. The kit comprises a pre-amplification reagent and an post-amplification reagent, wherein the pre-amplification reagent comprises a PCR (Polymerase Chain Reaction) buffer solution, a reaction mixture of MgCl2 and dNTPs (Deoxy-Ribonucleoside Triphosphates), a Taq enzyme, ultrapure water, and a primer mixture of a high-specificity amplification ACE gene; and the postr-amplification reagent comprises a genotyping reference substance and an internal label. The gene is taken as a detection object, and PCR amplification, capillary electrophoresis detection and comparison with the genotyping reference substance are performed, so that genotyping can be performed, an individual with a specific genotype is screened, and references are provided for physical material selection. On the aspect of physical material selection, a fluorescence labeling technology and a capillary electrophoresis technology are adopted for the first time to realize the high-sensitivity specific detection on a motor-function-related gene, so that manpower, materials and time are saved greatly.

Description

technical field [0001] The invention relates to a motor function-related gene ACE fluorescence detection kit and a detection method, belonging to the technical field of biological detection. Background technique [0002] With the completion of the Human Genome Project and the full development of the Post-Genome Project, the application of genetic engineering-led biotechnology in the field of sports has received widespread attention. The application of genetic engineering to athlete selection is a historical necessity and the trend of the times. It will fundamentally cause a revolution in the theory and methods of sports selection. Preliminary progress has been made in sports selection using the theory and methods of genetics. [0003] At present, it is generally believed that the level of ACE (angiotensin-converting enzyme) is closely related to the morphology and function of the cardiovascular system. The polymorphism of the ACE gene is one of the decisive factors for the...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 步迅夏子芳
Owner 山东百福基因科技有限公司
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