Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method

A fluorescence detection and kinesiology technology, applied in fluorescence/phosphorescence, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of cumbersome operation, difficult interpretation of results, poor repeatability, etc., saving manpower, material resources and time. Effect

Active Publication Date: 2014-11-19
山东百福基因科技有限公司 +4
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have different defects, such as cumbersome operation, difficult interpretation of results, poor repeatability, many false negatives and false positives, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method
  • Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method
  • Fluorescence test kit for motor-function-related gene ACE (Angiotensin Converting Enzyme) and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The kit of the present invention detects the DNA samples of 40 different individuals. The primers used for the detection of motor function-related genes were labeled with blue fluorescent dye, and the internal standard was labeled with red fluorescent dye.

[0030] 1. 40 samples to be tested, the results of 3 individuals with different genotypes are listed below. Relevant samples have been sequenced and tested for ACE using the technical method of "DNA extraction-PCR amplification-sequencing". Among them, the typing results of samples No. 71-2, No. 74-2, and No. 69-4 were: D / D, I / I, I / D.

[0031] 2. Genomic DNA extraction from samples

[0032] Chelex extraction method: Cut off 1-3mm blood spot and put it in a 1.5mL centrifuge tube, add sdH 2 O 1mL, shake and centrifuge, discard the supernatant, repeat the steps twice, discard the supernatant, shake and suspend 5% Chelex-100, quickly draw 200μL into the centrifuge tube with the tip of the cut pipette, and shake for a ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fluorescence test kit for a motor-function-related gene ACE (Angiotensin Converting Enzyme) and a detection method, belonging to the technical field of biological detection. The kit comprises a pre-amplification reagent and an post-amplification reagent, wherein the pre-amplification reagent comprises a PCR (Polymerase Chain Reaction) buffer solution, a reaction mixture of MgCl2 and dNTPs (Deoxy-Ribonucleoside Triphosphates), a Taq enzyme, ultrapure water, and a primer mixture of a high-specificity amplification ACE gene; and the postr-amplification reagent comprises a genotyping reference substance and an internal label. The gene is taken as a detection object, and PCR amplification, capillary electrophoresis detection and comparison with the genotyping reference substance are performed, so that genotyping can be performed, an individual with a specific genotype is screened, and references are provided for physical material selection. On the aspect of physical material selection, a fluorescence labeling technology and a capillary electrophoresis technology are adopted for the first time to realize the high-sensitivity specific detection on a motor-function-related gene, so that manpower, materials and time are saved greatly.

Description

technical field [0001] The invention relates to a motor function-related gene ACE fluorescence detection kit and a detection method, belonging to the technical field of biological detection. Background technique [0002] With the completion of the Human Genome Project and the full development of the Post-Genome Project, the application of genetic engineering-led biotechnology in the field of sports has received widespread attention. The application of genetic engineering to athlete selection is a historical necessity and the trend of the times. It will fundamentally cause a revolution in the theory and methods of sports selection. Preliminary progress has been made in sports selection using the theory and methods of genetics. [0003] At present, it is generally believed that the level of ACE (angiotensin-converting enzyme) is closely related to the morphology and function of the cardiovascular system. The polymorphism of the ACE gene is one of the decisive factors for the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68G01N21/64
Inventor 步迅夏子芳
Owner 山东百福基因科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com