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Deafness susceptive gene joint detection kit

A susceptibility gene, combined detection technology, applied in the field of genes, can solve the problems of high precision, high price, and inconsistent supporting analysis software.

Active Publication Date: 2014-03-26
潮州凯普生物化学有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex production process of the gene chip technology, the high precision required, it is difficult for ordinary laboratories to meet the requirements, and the supporting analysis software is not uniform, etc., and because it has just entered industrialization, the price is relatively expensive. These shortcomings make the application of this technology subject to certain restrictions. Restrictions, unable to meet the requirements of clinical rapid detection

Method used

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  • Deafness susceptive gene joint detection kit
  • Deafness susceptive gene joint detection kit
  • Deafness susceptive gene joint detection kit

Examples

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Embodiment Construction

[0103] The present invention will be described in detail below through examples.

[0104] In the examples, 20% EDAC solution, 0.1% SDS, 0.25% skimmed milk powder, 0.05% thimerosal, and 0.05% sodium azide all refer to the mass ratio, and 0.1% Tween20 refers to the volume ratio.

[0105] Step 1 Preparation of gene chip for detecting deafness susceptibility

[0106] For 4 common mutation sites in mtDNA gene (1494 C>T, 1555 A>G, 7445 A>G, 12201 T>C); 5 common mutation sites in GJB2 gene (35(-G), 155( -TCTG), 176 (del 16bp), 235 (-C), 299 (-AT); 1 common mutation site in GJB3 gene (538 C>T); 3 common mutation sites in SLC26A4 gene (IVS7-2 A>G, 1229 C>T, 2168 A>G), a total of 26 probes were designed, the probe sequence is SEQ ID Nos: 1-26, and the length of the probe is generally about 14-25 bases. The 5' or 3' end of the needle is aminated.

[0107] The production steps of the DNA gene chip are as follows: the prepared probe and Bio were first dissolved in ultrapure water to mak...

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Abstract

The invention discloses a deafness susceptive gene joint detection kit which comprises (1) a gene chip and (2) various primers, wherein the gene chip is provided with (i) nucleotide sequence probes and (ii) a DNA (deoxyribonucleic acid) sequence marked with biotin sites; the nucleotide sequence probes are at least one of sequences shown as SEQ ID Nos:1-26 and one of sequences complementary with the sequences shown as SEQ ID Nos:1-26; the DNA sequence marked with the biotin sites is as shown in SEQ ID No.27; and the primers are respectively shown as SEQ ID No.28-29, SEQ ID No.30-31, SEQ ID No.32-37 and SEQ ID No.38-43. The kit disclosed by the invention can realize synchronous joint detection, enhance the detection specificity, reduce the cost and shorten the detection time.

Description

technical field [0001] The invention relates to the field of genes, in particular to a combined detection kit for deafness susceptibility genes. Background technique [0002] Deafness is a common congenital disease that seriously affects the quality of human life. It can be caused by a single gene mutation or a compound mutation of different genes, or by environmental factors (such as medical factors, environmental exposure, trauma, drugs, etc.) or genetic and environmental factors. Both work together. Worldwide, there is one child with congenital deafness in every 1,000 newborns, and 50% of the children's deafness is related to genetic factors. According to statistics from the website of the China Disabled Persons' Federation, 21 million of China's 60 million disabled people are hearing disabled. Among those with hearing and speech disabilities, there are 800,000 deaf children under the age of 7, and the number of deaf children is increasing at an annual rate of 30,000. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B40/06
Inventor 朱娟娟谢龙旭李烈军
Owner 潮州凯普生物化学有限公司
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