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Preparation of low-crystalline nano hydroxyapatite through membrane modulation and mineralization method

A technology of hydroxyapatite and diaphragm, applied in chemical instruments and methods, phosphorus compounds, inorganic chemistry, etc., can solve the problems of slow reaction degradation, inconvenient management of manufacturers, and difficulty in rebuilding bones, etc.

Active Publication Date: 2014-08-20
TIANJIN SANNIE BIOENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are basically two methods for preparing hydroxyapatite. One is chemical synthesis and high-temperature calcination. The hydroxyapatite obtained by this method has a high degree of crystallinity, and the clinical multi-reaction degradation rate is too slow, making it difficult to rebuild bones; the other is animal collagen. Protein co-precipitation method is biomimetic. The hydroxyapatite obtained by this method has low crystallinity, which is conducive to degradation and absorption in the body. However, the material obtained by this method is an active material, and the procurement of raw materials is very strict, which brings a lot of management to the manufacturer. inconvenience, which increases the cost of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Measure 100 ml of collagen solution with a concentration of 0.5%, put it into a dialysis bag with a molecular weight cut-off of 1000, and the concentration of hydrochloric acid is 0.01 mol / L. At the same time, measure 500ml of 0.02mol / L calcium chloride solution, drop 0.38ml of concentrated phosphoric acid into it, and finally weigh 0.77g of sodium hydroxide and pour it into the mixed solution of calcium chloride and phosphoric acid, and stir rapidly with electric power. And put into a dialysis bag filled with collagen solution. Stir for 12 h at a temperature of 23 °C, and take out the dialysis bag. Finally, the obtained white solution is filtered through a 0.45 μm membrane to obtain low-crystalline nano-hydroxyapatite. The mass ratio of collagen to calcium chloride is 1:2.2; the mass ratio of calcium chloride to sodium hydroxide is 10:7.

Embodiment 2

[0014] Measure 100 ml of collagen solution with a concentration of 5.0%, put it into a dialysis bag with a molecular weight cut-off of 1000, and the concentration of hydrochloric acid is 0.2 mol / L. At the same time, measure 500ml of 0.02mol / L calcium chloride solution, drop 0.38ml of concentrated phosphoric acid into it, and finally weigh 1.0g of sodium hydroxide and pour it into the mixed solution of calcium chloride and phosphoric acid, and stir rapidly with electric power. And put into a dialysis bag filled with collagen solution. Stir for 24 h at a temperature of 10 °C, and take out the dialysis bag. Finally, the obtained white solution is filtered through a 0.45 μm membrane to obtain low-crystalline nano-hydroxyapatite. The mass ratio of collagen to calcium chloride is 5:1; the mass ratio of calcium chloride to sodium hydroxide is 1.1:1.

Embodiment 3

[0016] Measure 100 ml of collagen solution with a concentration of 10.0%, put it into a dialysis bag with a molecular weight cut-off of 1000, and the concentration of hydrochloric acid is 0.5 mol / L. At the same time, measure 400 ml of 2.0 mol / L calcium chloride solution, drop 30.4 ml of concentrated phosphoric acid into it, and finally weigh 77.0 g of sodium hydroxide and pour it into the mixed solution of calcium chloride and phosphoric acid, and stir quickly with electric power. And put into a dialysis bag filled with collagen solution. Stir for 0.5 h at a temperature of 8 °C, and take out the dialysis bag. Finally, the obtained white solution is filtered through a 0.45 μm membrane to obtain low-crystalline nano-hydroxyapatite. The mass ratio of collagen to calcium chloride is 1:8.8; the mass ratio of calcium chloride to sodium hydroxide is 8:7.

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PUM

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Abstract

The invention provides a material which can be used for an artificial bone and a drug carrier and a preparation method thereof, belongs to the field of medical biological materials, mainly solves the problems that the hydroxyapatite produced through high-temperature calcination in clinical use at present is high in crystalline and is nondegradable and active raw materials of the bionic hydroxyapatite are difficult to control, and solves the problem on a favorable drug carrier for preventing and treating osteoporosis. The osteogenesis-inducing hydroxyapatite is provided to a receptor, and after implantation, medical fluorine is injected to achieve the purposes of helping bone healing and preventing and treating osteoporosis. The material prepared through the method is prepared from dialysis bag isolation collagen having a certain molecular weight cutoff and inorganic calcium salt through a method that the collagen arranged inside a dialysis membrane is used to modulate and mineralize calcium ions outside the dialysis membrane based on the characteristic that the molecular weight of the collagen is large, and the material is stable, uniform, low in crystalline and favorable in biocompatibility. Thus, the invention is beneficial to bone healing of patients with osteonosus.

Description

technical field [0001] The invention relates to a low-crystalline nano-hydroxyapatite produced by modulation and mineralization of a collagen membrane. This product is low crystalline nanoscale. Can be used for artificial bone and drug carrier. It belongs to the field of medical biomaterials. Background technique [0002] The main raw material of artificial bone currently on the market is hydroxyapatite. It has attracted wide attention due to its inductive effect on osteogenesis and its good biocompatibility. There are basically two preparation methods for hydroxyapatite. One is chemical synthesis and high-temperature calcination. The hydroxyapatite obtained by this method has high crystallinity, and the degradation rate of clinical multiple reactions is too slow, making it difficult to rebuild bones; the other is animal collagen. The hydroxyapatite obtained by this method has a low crystallinity, which is beneficial to degradation and absorption in vivo. However, the ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B25/32A61K47/02A61L27/12
Inventor 毕宏伟张春红王宗龙
Owner TIANJIN SANNIE BIOENG TECH