Volatile organic product preparation method

A volatile organic, product technology, applied in the field of biochemical industry, can solve problems to be improved, etc.

Inactive Publication Date: 2013-01-30
ACS农化系统有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the means of producing volatile organic products by microbial fermentation still needs to be improved.

Method used

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  • Volatile organic product preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Production process such as Figure 4 As shown, three fermenters (3, 3' and 3") are cascaded for continuous fermentation process. Feed stream 1 contains 27% glucose and 0.2% non-fermentable matter (mainly mineral salts) with a flow rate of 1000kg / h. The total fermentation volume of the three fermenters is 40m 3. The microorganisms used are genetically engineered yeasts of the genus Arxula disclosed in European patent application EP 11161243.8 (such as Arxula adeninivorans ATCC 76597). When the fermentation process reaches a steady state, the last stage fermenter (fermenter 3") contains 4% n-butanol, 4% yeast cells and about 0.3% non-fermentable matter. Stream 11 is taken from the bottom of fermenter 3". out, enter the membrane separator 13, and obtain the retentate 8 and the permeate 4 after being filtered by the ultrafiltration membrane 12. The permeate 4 further enters the membrane separator 5 and is separated by the lipophilic pervaporation membrane 6 to obtain th...

Embodiment 2

[0045] Production process such as Figure 5 shown. Continuous fermentation using a cascade of three fermenters (3, 3’ and 3”) with a total fermentation volume of 40m 3 . Feed stream 1 contained 27% glucose and 0.2% non-fermentable matter (mainly mineral salts) and had a flow rate of 1700 kg / h. The target product is ethanol, and the microorganism used is Saccharomyces cerevisiae. When steady-state fermentation is achieved, the concentration of ethanol in the third-stage fermenter 3" is 9-10%, the concentration of yeast cells is 6%, the concentration of non-fermentable substances is about 0.3%, and almost all glucose is converted into ethanol. Fermenter 3 "The bottom is produced stream 11 with about 3200kg / h flow rate. Stream 11 flows into ultrafiltration membrane separator 13 and is separated into retentate 8 and permeate 4 by ultrafiltration membrane 12 . The retentate 8 mainly contains water, yeast cells, and macromolecular non-fermentable substances, while the permeate ...

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Abstract

The present invention provides a method for continuous fermentation and purification of a volatile organic product, and particularly relates to a method for production, separation, concentration and purification of a volatile organic product from a fermentation broth through a membrane separation process, wherein the membrane separation process is a combination comprising one or a plurality of processes selected from pervaporation, vapor penetration, micro-filtration, ultra-filtration and nano-filtration. With the method, concentration of a target organic product can be maintained to a low level during a fermentation process so as to avoid inhibition on the fermentation process; and after the oleophilic pervaporation membrane separation is performed, concentration of the target product in the penetrant is increased so as to reduce subsequent product concentration and purification energy consumption. The method further has an advantage that: the product concentration is controlled to a non-inhibitory concentration level during the fermentation process, such that the whole process can be subjected to a continuous operation.

Description

technical field [0001] The invention relates to the field of biochemical industry, and more specifically, the invention relates to a method for preparing volatile organic products through fermentation. Background technique [0002] With the development of bioengineering, more and more high value-added products can be produced by fermentation of microorganisms, such as bacteria and yeast, using a variety of substrates. The target product may be an intracellular accumulation product, and the fermentation process needs to increase the cell biomass as the main goal. Another situation is that the target product is secreted outside the cell during the growth and metabolism of the bacteria, that is, secreted into the fermentation broth. At this time, the growth of the biomass of the cells after a certain period of growth needs to be limited to ensure that the fermentation process of the second stage The optimal yield of the target product can be obtained under the premise of no ob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/02C12P7/06C12P7/16B01D61/00B01D61/58C07C31/08C07C31/12C07C29/76
CPCY02E50/17Y02E50/10
Inventor 埃里克·F·图梭哈特穆特·布鲁斯克刘德华赵雪冰
Owner ACS农化系统有限公司
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