Method for measuring content of polysaccharide in camellia seeds

A technology of polysaccharide content and determination method, applied in the field of polysaccharide content determination, can solve problems such as increasing difficulty and affecting the accuracy of polysaccharide content determination, and achieves the effects of high reproducibility, strong operability and accurate determination results.

Inactive Publication Date: 2013-02-06
伊国群
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Problems solved by technology

However, since camellia oleifera seeds also contain camellia oil, tea saponin, flavonoids and other active ingredients, it is more difficult to effectively extract polysaccharides with higher purity from camellia oleifera seeds, and affects the accuracy of polysaccharide content determination. Spend

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for assaying polysaccharide content in camellia oleifera seeds, comprising the following steps:

[0027] (1) Accurately weigh 5g of Camellia oleifera seed sample, wrap it with double-layer filter paper, place it in a Soxhlet extractor, add 80ml of petroleum ether to a round bottom flask, heat and reflux in an oil bath at a temperature of 70°C, and extract After 4 hours, the camellia seed I and petroleum ether extract were obtained, the camellia seed I was retained, and the petroleum ether extract was discarded;

[0028] (2) Add 80ml of 70% ethanol to the round-bottomed flask containing the above-mentioned Camellia oleifera I, heat and reflux in an oil bath at a temperature of 90°C, and extract for 4 hours to obtain Camellia oleifera II and ethanol extract, discard Ethanol extract, the camellia oleifera II is taken out, and dried naturally in a dark and ventilated place;

[0029] (3) Wrap the dried Camellia oleifera II in step (2) with double-layer filter paper...

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Abstract

The invention provides a method for measuring the content of polysaccharide in camellia seeds. The method comprises the following steps of: firstly, sequentially extracting camellia seeds by using a low-polarity solvent and alcohol, removing liposoluble substances and soluble glycoside substances from the camellia seeds; extracting polysaccharide from the camellia seeds by using water; and finally measuring the content of the polysaccharide in the camellia seeds by adopting a sulfuric acid-phenol method. The method provided by the invention has the advantages of strong maneuverability, accuracy in measuring results and high reproducibility, can be carried out in common conventional routine test laboratories, and provides an effective guarantee for development, production and quality control of polysaccharide products.

Description

technical field [0001] The invention relates to a method for determining the content of polysaccharides, in particular to a method for determining the content of polysaccharides in camellia oleifera seeds. Background technique [0002] Camellia oleifera, commonly known as bitter tea, Jiancha, and white flower tea, has become one of the excellent edible vegetable oils because it can extract camellia oil with an unsaturated fatty acid content as high as 93% from its seeds. At the same time, studies have shown that Camellia oleifera seeds also contain bioactive active ingredients such as polysaccharides, tea saponin, flavonoid glycosides and caffeine, which have high research and application value. [0003] Plant water-soluble polysaccharides are natural active ingredients isolated from natural products. They have a variety of biological activities and pharmacological effects, mainly including: enhancing immunity, anti-tumor, lowering blood pressure, lowering blood fat, lowerin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/34G01N1/38
Inventor 伊国群孟庆华
Owner 伊国群
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