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Rectum exudate heme test method and rectum exudate heme test kit

A technology of heme and exudate, which is applied in the field of detection of heme in rectal exudate, can solve the problems of long cycle, high cost, and complicated operation, and achieve the effects of low cost, reduced labor burden, and high sensitivity

Active Publication Date: 2014-06-11
青岛中联海诺医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of high cost, long period, and complicated operation in the prior art method for detecting hemoglobin in rectal exudate, the present invention provides a method for detecting hemoglobin in rectal exudate, which has high sensitivity, low cost, and rapid detection of hemoglobin in rectal exudate; at the same time, the invention also provides a special detection box for detecting heme in rectal exudate

Method used

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  • Rectum exudate heme test method and rectum exudate heme test kit
  • Rectum exudate heme test method and rectum exudate heme test kit
  • Rectum exudate heme test method and rectum exudate heme test kit

Examples

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Embodiment 1

[0023] A method for detecting hemoglobin in rectal exudate, the method is as follows: slide open the sliding cover 1 of the detection box, take out the finger cot 6 from the box body 8, put on latex gloves, and then put the finger cot 6 on the first joint of the index finger On the rectum, wipe with a little pressure to obtain the exudate, wherein the exudate volume is 0.5-1 ml; take the sample cup 7 in the sample cup placement area 3, and put it into tris-hydrochloric acid with a pH value of 7.4 3 ml of buffer solution, put the finger cot 6 with seepage into the sample cup 7, and mix well; take out two droppers from the two strip grooves 2, and the two droppers are pre-filled with reagent A and reagent B, sequentially add 1ml of sample solution, 0.5ml of reagent A, and 0.5ml of reagent B into the test pool 4, mix well, observe the color change within 2 minutes, compare and record the test results with the color guide 5; the preparation of the reagent A The method is ...

Embodiment 2

[0030] Combine below Figure 1 to Figure 4 The special detection box of the present invention is further introduced.

[0031] The detection box for detecting rectal exudate hemoglobin provided by the present invention comprises a box body 8 and a sliding cover 1 slidingly connected with the box body 8, the sliding cover 1 can slide along the direction of the upper surface of the box body 8, and the sliding cover 1 is provided with There are two bar-shaped grooves 2 protruding outward, a dropper is housed in the bar-shaped groove 2, the bar-shaped groove 2 is sealed from the bottom, the inside of the box body 8 is hollow, and one side of the box body 8 is provided with a color comparison plate 5, The inner bottom of the box body 8 is also provided with a test pool 4 and a sample cup placement area 3 for placing the sample cup 7. Finger cots 6 are placed on the 4 sides of the test pool. The test pool 4 extends inward from the bottom of the box body 8, and the middle part is ...

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Abstract

Provided is a method for detecting haemoglobin in rectal exudate, comprising: wiping at the site of recta under slight pressure to obtain exudate; treating the exudate with a trimethylol aminomethane-hydrochloric acid buffer of PH 7.4; reacting with a chromogenic reagent and performing color comparison with a colorimetric plate; and recording detection results. Also provided is a kit for detecting haemoglobin in rectal exudate.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting hemoglobin in rectal exudate, and at the same time, the invention also relates to a special detection box for detecting hemoglobin in rectal exudate. Background technique [0002] The hemoglobin in the rectal exudate is often caused by the release of hemoglobin from the tissue cell oxygen sensor and FixL transmembrane protein, the hemoglobin precipitation of the tumor cells, or the exudation of the microcirculation. Generally, it is necessary to use advanced instruments and analytical chemical methods for detection, which is costly, long-term, and complicated to operate. Therefore, it is an urgent need to invent a detection method with simple operation and low cost to quickly determine whether the rectal exudate contains hemoglobin. solved problem. Contents of the invention [0003] In order to solve the problems of high cost, long period and complicated operation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N33/72
Inventor 温鑫崔子伦杜晓丽
Owner 青岛中联海诺医疗科技有限公司