Potato circulating production process by means of detoxified potato test tube

A production process and test-tube tuber technology, applied in the field of virus-free potato test-tube tuber production, can solve the problems of long production cycle, many production processes, increased waste of resources and environmental pollution, etc., achieve uniform growth, simple production process, shorten The effect of the production cycle

Inactive Publication Date: 2013-02-13
达州市农业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that there are many production processes (three stages); the production cycle is long (about 110 days); the potato tube seedlings can only be produced once, and cannot be recycle

Method used

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  • Potato circulating production process by means of detoxified potato test tube
  • Potato circulating production process by means of detoxified potato test tube
  • Potato circulating production process by means of detoxified potato test tube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take test-tube seedlings with 4 to 5 stem nodes, cut into single stem nodes under sterile conditions, and inoculate on the strong seedling medium, the light time is 16h·d -1 , Light intensity 2500 ~ 3000lx. After 3 to 4 weeks, enough test-tube seedlings with the same source were induced, and the test-tube seedlings were subjected to the following 5 treatment methods using the induction medium formula and inoculation method (see Table 1), first cultivated in light for 5 days, and then transferred to Cultivate induced tube tubers in a dark room at 15-20°C. 10 bottles were treated each time, with 20 plants per bottle, repeated 3 times. After 35 days of treatment, the number of tuber tubers was investigated, and after 55 days, the total number of tubers, the number of large tubers and the grain weight were investigated. (Standard: large potato d>5mm; knot potato d>3mm). The test results are shown in Table II.

[0027] Table 1. Processing method

[0028]

[0029] (In...

Embodiment 2

[0036] The applicant selects the test-tube seedlings of the potato variety Qinyu No. 30 that has passed the virus-free detection. According to the design method of the application, the test-tube seedlings are subtracted from the top buds, and the test-tube tuber induction medium is added to induce tuber formation. The clipped top buds are used As a basic seedling breeding, a total of 820 bottles of induced test tube tubers were produced, harvested after 2 and a half months, a total of 20090 tubers, with a total weight of 2034 grams, an average of 24.5 tubers and 2480.5 mg per bottle.

Embodiment 3

[0038] Utilize the test-tube plantlets propagated by terminal buds in Example 2, cut off the terminal buds and add an induction medium to induce tuber formation, and the clipped terminal buds are used as basic seedlings for breeding, and a total of 2000 bottles of induced test-tube tubers are produced. The temperature was controlled at 15-18°C for the first 20 days of tuber induction, and 20-22°C for the later period. After 2 months, a total of 56,780 tubers were harvested, with a total weight of 6,485 grams, and an average of 28.3 tubers and 3,242.5 mg per bottle.

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Abstract

The invention discloses a potato circulating production process by means of a detoxified potato test tube. The potato circulating production process comprises the following steps of test-tube strong seedling cultivation and test-tub potato induction. After the step of the test-tube strong seedling cultivation is performed, terminal buds of detoxified potato test-tube strong seedlings cultured in the step of the test-tube strong seedling cultivation are sheared, and then the detoxified potato test-tube strong seedlings without the terminal buds are added into a test-tub potato induction culture medium to perform test-tub potato induction production. Sheared terminal buds are continuously used for the test-tube strong seedling cultivation. The potato circulating production process is optimized while improving the production quantity per unit area and grain weight of test-tub potatoes, shortens production procedures, reduces the production cost, achieves annual reciprocating circulation to promote the detoxified potato production by means of the detoxified potato test tube, accelerates breeding, popularization and application of the detoxified potatoes, and accordingly promotes improvement of the production level of potatoes in our country and quick development of potato industry.

Description

technical field [0001] The invention relates to the field of production of virus-free potato test-tube potatoes, in particular to a circulating production process of virus-free potato test-tube potatoes. Background technique [0002] China is the most populous country in the world, and the contradiction between man and land is prominent. The food issue is crucial. Potato is a rare high-yield crop in the world. It has excellent characteristics such as comprehensive nutrition, wide adaptability, strong stress resistance, high and stable yield, long industrial chain, and both food and vegetable feed industrial raw materials. It is the fourth largest food crop in the world and the second largest in China. five major food crops. China's potato planting area and total output rank first in the world, but the yield per unit is relatively low. The average yield per unit is 956.5kg / 666.67 square meters, ranking 82nd in the world, and about 1 / 3 of the world's advanced developed count...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 胡振兴赵思毅张玲李薇杨小丽刘晓英贾荟芹
Owner 达州市农业科学研究所
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