Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing high purity monounsaturated fatty acid

An unsaturated fatty acid, high-purity technology, applied in the field of preparing high-purity monounsaturated fatty acid isomers, to achieve high product purity, simple experimental equipment, and cost-saving effects

Inactive Publication Date: 2014-11-12
NAT INST OF METROLOGY CHINA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Studies have found that cis-monounsaturated fatty acids have important physiological functions, such as significantly lowering cholesterol, reducing the incidence of cardiovascular and cerebrovascular diseases, repairing neuron cells, etc., and have been widely used in pharmaceuticals and health food industries. However, it is difficult to obtain monounsaturated fatty acids with a content of more than 99% from the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing high purity monounsaturated fatty acid
  • Method for preparing high purity monounsaturated fatty acid
  • Method for preparing high purity monounsaturated fatty acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, the preparation of cis oleic acid and trans oleic acid

[0067] Accurately weigh 50g of oleic acid raw material (it has been determined to contain 40g of cis-oleic acid and 5g of trans-oleic acid) into a beaker, add 100mL of methanol, and prepare the oleic acid raw material sample into a methanol solution with a concentration of 0.5g / mL.

[0068] Inject the prepared sample evenly and slowly into the upper layer of the C18 low-pressure preparation column through a pipette, open the stopcock of the chromatography column, so that the sample is evenly and fully adsorbed on the C18 filler, and use the mobile phase to elute after the adsorption is completed.

[0069] The mobile phase was a mixed solution of methanol / water (95:5, V / V) containing 0.1% formic acid, isocratic elution, and the flow rate was 3 mL / min.

[0070] A fraction collector is used for automatic collection, and the collection volume of each fraction is 150ml.

[0071] Color analysis was carrie...

Embodiment 2

[0074] Embodiment 2, preparation of high-purity cis-arachidic acid

[0075] Accurately weigh 50g of peanut oleic acid raw material (measured to contain 37.5g of peanut oleic acid) into a beaker, and add 100mL of methanol to make the concentration of peanut oleic acid sample 0.5g / mL.

[0076] Inject the prepared sample evenly and slowly into the upper layer of the C18 low-pressure preparation column through a pipette, open the stopcock of the chromatography column, so that the sample is evenly and fully adsorbed on the C18 filler, and use the mobile phase to elute after the adsorption is completed.

[0077] The mobile phase is a methanol solution containing 0.1% formic acid, isocratic elution, and the flow rate is 3 mL / min.

[0078] A fraction collector is used for automatic collection, and the collection volume of each fraction is 150ml.

[0079] Color analysis was carried out on the collected eluate fractions by bromocresol green color reaction, and the fractions reacted wit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a method for preparing a cis-isomer and a trans-isomer of high purity monounsaturated fatty acid. According to the present invention, a low pressure reverse phase C18 column chromatography technology is firstly adopted to carry out separation purification on a monounsaturated fatty acid mixture crude product. With the method, preparation of hundred gram-scale to kilogram-scale of high purity monounsaturated fatty acids can be achieved, and resolution of the cis-isomer and the trans-isomer of the monounsaturated fatty acid can be achieved. In addition, the method has characteristics of simple laboratory equipment, easy operation and cost saving.

Description

Technical field: [0001] The invention belongs to the field of food, and in particular relates to a method for preparing high-purity monounsaturated fatty acid, especially a method capable of preparing high-purity monounsaturated fatty acid isomers. Background technique: [0002] Monounsaturated fatty acids refer to fatty acids containing a double bond in their molecular structure, and their types and sources are extremely rich, such as myristoleic acid, palmitoleic acid, oleic acid, peanut oil Eicosenoic acid, cis-trans isomers of erucic acid (Docosenoic acid), etc. [0003] In food, chemical, pharmaceutical and other industries, it is often necessary to use high-purity monounsaturated fatty acids as raw materials and determination standards. There have been many literature reports on the extraction and preparation of fatty acids, such as: [0004] T.C.Marquardt, R.F.Wilson.An improved reversed-phase thin-layer chromatography method for separation of fatty acid methyl este...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/22B01D15/10
Inventor 盛灵慧高运华黄峥李佳马康王晶李良
Owner NAT INST OF METROLOGY CHINA