Culture medium for improving hydrogen production amount of Chlamydomonas reinhardtii, and its preparation method

A culture medium and the technology of Chlamydomonas, applied in the field of culture medium and its preparation for increasing the hydrogen production of Chlamydomonas chlamydomonas, can solve the problems that cannot meet the requirements of practical application, etc.

Active Publication Date: 2013-02-13
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although more hydrogen can be obtained under this culture condition, it still cannot meet the requirements of practical application.

Method used

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  • Culture medium for improving hydrogen production amount of Chlamydomonas reinhardtii, and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1, algal species CC400 hydrogen production detection

[0046] 1. Materials

[0047] Chlamydomonas reinhardtii, strain CC400 was purchased from Duke University strain Chlamydomonas reinhardtii (Chlamy center, http: / / www.chlamy.org / );

[0048] Two, medium (various components in the following medium can be purchased from commercial sources)

[0049] 1. Normal TAP medium: NH 4 Cl 0.4g / L; MgSO 4 ·7H 2 O 0.1g / L; CaCl 2 2H 2 O 0.05g / L; K 2 HPO 4 0.108g / L; KH 2 PO 4 0.056g / L; Tris base 2.423g / L; Hunter’s trace elements 1ml / L, glacial acetic acid 1ml / L, and the rest is water.

[0050] Hunter’s trace elements: H 3 BO 4 11.4g / L, ZnSO 4 ·7H 2 O 22.0g / L, MnCl 2 4H 2 O 5.06g / L, CoCl 2 ·6H 2 O 1.61g / L, CuSO 4 ·5H 2 O 1.57g / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 1.10g / L, FeSO 4 ·7H 2 O 4.99g / L, the rest is water.

[0051] Solid TAP medium: add 1.5% agar powder to normal TAP medium.

[0052] The pH value of the medium was all 7.

[0053] 2. Sulfur-defi...

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Abstract

The invention discloses a culture medium for improving the hydrogen production amount of Chlamydomonas reinhardtii, and its preparation method. The culture medium provided by the invention is composed of NH4Cl, MgCl2.6H2O, K2HPO4, KH2PO4, a Tris base, H3BO4, ZnCl2, MnCl2.4H2O, CoCl2.6H2O, CuCl2.2H2O, (NH4)6Mo7O24.4H2O, FeCl2.4H2O and glacial acetic acid. Experiments in the invention prove that the hydrogen production amount of the Chlamydomonas reinhardtii in the sulfur-lack and calcium-lack TAP culture solution is about 2 / 3 higher than the hydrogen production amount of the Chlamydomonas reinhardtii in present sulfur-lack systems after optimizing the formulas of present hydrogen desorption culture mediums (sulfur-lack TAP culture solutions), and a culture medium component is omitted, so the cost is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium for increasing the hydrogen production of Chlamydomonas chlamydomonas and a preparation method thereof. Background technique [0002] Due to the depletion of mineral resources, looking for clean alternative energy has become an urgent issue. Hydrogen is the simplest and most abundant element in the universe. Its calorific value is as high as 118.4kJ / g, and only water is produced after combustion. It is generally considered to be the most attractive alternative energy source. New hydrogen production methods, especially those that can utilize renewable energy and produce hydrogen in large quantities, have attracted much attention. [0003] Photosynthetic hydrogen production by algae is a biological process in which microalgae use solar energy to split water to release hydrogen, and it is one of the important ways to achieve sustainable hydrogen production. In order t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/89
Inventor 黄芳赵磊
Owner INST OF BOTANY CHINESE ACAD OF SCI
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