Multi-antibody marked quick procalcitonin detecting kit

A detection kit and multi-antibody labeling technology, applied in the detection field, can solve the problems of inability to directly detect whole blood, unsuitable for rapid diagnosis, complicated operation process, etc., to promote timely and accurate diagnosis and standardized application of antibiotics, to achieve sensitivity and The effect of high specificity detection and improved detection sensitivity

Active Publication Date: 2013-02-13
WUHAN EASYDIAGNOSIS BIOMEDICINE
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AI Technical Summary

Problems solved by technology

[0007] Chinese invention patent application CN101029897A (public date: 2007.9.5) discloses a test kit for procalcitonin and its test method. The method uses nano-immunomagnetic microbeads as a separation reagent, and anti-PCT monoclonal antibody as a luminescent marker Labeled isoluminol derivatives, using the PCT kit and testing method thereof according to the present invention, based on the characteristics that the detection sensitivity of the chemiluminescence method can reach the pg/ml level

Method used

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Embodiment

[0029] Example, the rapid detection kit for procalcitonin labeled with multiple antibodies using colloidal gold as a marker

[0030] Step 1, determine the capture antibody and labeled antibody

[0031] (1) Select two monoclonal antibodies targeting the N-terminal region of procalcitonin, or select a monoclonal antibody and a polyclonal antibody, mix them and immobilize them on a cellulose membrane as a capture antibody, and select multiple Monoclonal or polyclonal antibodies against the calcitonin region and the C-terminal region are used as labeled antibodies; the aforementioned monoclonal antibodies and polyclonal antibodies can be obtained through public channels;

[0032] (2) Label the monoclonal or polyclonal antibodies targeting the calcitonin region and the C-terminal region with colloidal gold, and spray on the gold label pad at a concentration of OD25, and dry at 37°C;

[0033] (3) Combination test with capture antibody, using conventional techniques to screen out t...

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Abstract

The invention relates to a multi-antibody marked quick procalcitonin detecting kit in the technical field of detection. The kit comprises a common carrier, a monoclonal or polyclonal capturing body which is fixed on the common carrier and corresponding to different procalcitonin areas, and a monoclonal or polyclonal capturing body which is compatible with the capturing body and is suitable for a plurality of same or different areas for the procalcitonin marked by a marker. According to the multi-antibody marked quick procalcitonin detecting kit, different distinguishing areas with multi-antibody pair coated are marked and the spaces are subjected to compatibility of medicines, the procalcitonin in a sample can be captured to the greatest extent, and the sensitivity in detection can be improved; the detection sensitivity of PCT (procalcitonin) is improved to 0.1ng/ml by the kit, so that the application value and the application range of a quick PCT detecting agent in diagnosis of bacterial infection, differential diagnosis and treatment can be increased and expanded; and the kit can be applied to diagnosis of bacterial infection, differential diagnosis, treatment effect monitoring and post-curing assessment.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a multi-antibody-labeled procalcitonin rapid detection kit. Background technique [0002] In 1993, Lancet reported for the first time that the level of procalcitonin (precalcitonin, PCT) was significantly increased in sepsis. When the blood PCT concentration is greater than 0.5ng / ml, it indicates the possibility of sepsis. If it is greater than 2ng / ml, it indicates the existence of sepsis. If it is greater than 10ng / ml indicates severe sepsis or shock, and PCT can be used as a specific indicator for the diagnosis of sepsis. [0003] In recent years, with the deepening of research, people have gradually found that the low level of PCT (0.1ng / ml-0.5ng / ml) has been increased in the early stage of bacterial infection, and the elevated level of PCT is related to the severity and scope of bacterial infection. Antibiotic therapy is recommended when PCT levels are greater...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 陈莉莉王颖
Owner WUHAN EASYDIAGNOSIS BIOMEDICINE
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