Application of phenylethanoid glycoside monomer compounds

A compound and drug technology, applied in the field of medicine, can solve problems such as unclear effects of phenylethanol glycoside monomers

Inactive Publication Date: 2013-02-20
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the Chinese patent with the application number 201110459715.X, it is disclosed that the phenylethanol glycosides in velvet grass have new uses in the prevention and treatment of cardiovascular diseases. However, it is unclear which phenylethanol glycoside monomers act

Method used

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  • Application of phenylethanoid glycoside monomer compounds
  • Application of phenylethanoid glycoside monomer compounds
  • Application of phenylethanoid glycoside monomer compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1, savatiside E

[0030] (1) Extraction of medicinal materials

[0031] 50kg of velvet antler medicinal material, cut into small pieces of 1-2cm, heated and refluxed with 15 times the amount of water to extract three times, each time for 2 hours. Combine the extracts, concentrate under reduced pressure at 70°C to 50L (relative density 1.15-1.18), add 250L of 95% ethanol, stir while adding, let stand overnight, take the supernatant, and concentrate under reduced pressure at 70°C to 50L.

[0032] (2) AB-8 resin purification

[0033] Resin dosage: medicinal material quality / resin quality=1:1;

[0034] Sample loading speed: 1mL / min.cm 2 ;

[0035] Elution rate: 2-3mL / min.cm 2 ;

[0036] The diameter-to-height ratio of the resin bed is 1:10;

[0037] Take 50 kg of the processed AB-8 resin, put it into a stainless steel chromatographic column with a diameter of 30 cm, wash it with water, and set it aside. The extract was loaded twice and ...

Embodiment 2

[0058] The preparation of embodiment 2, savatiside A

[0059] (1) Extraction of medicinal materials

[0060] 50kg of velvet antler medicinal material, cut into small pieces of 1-2cm, heated and refluxed with 15 times the amount of water to extract three times, each time for 2 hours. Combine the extracts, concentrate under reduced pressure at 70°C to 50L (relative density 1.15-1.18), add 250L of 95% ethanol, stir while adding, let stand overnight, take the supernatant, and concentrate under reduced pressure at 70°C to 50L.

[0061] (2) AB-8 resin purification

[0062] Resin dosage: medicinal material quality / resin quality=1:1;

[0063] Sample loading speed: 1mL / min.cm 2 ;

[0064] Elution rate: 2-3mL / min.cm 2 ;

[0065] The diameter-to-height ratio of the resin bed is 1:10;

[0066] Take 50 kg of the processed AB-8 resin, put it into a stainless steel chromatographic column with a diameter of 30 cm, wash it with water, and set it aside. The extract was loaded twice and ...

Embodiment 3

[0087] Embodiment 3, DPPH method measures the scavenging ability of phenylethanoid glycoside monomer compound to free radical

[0088] The savatiside E prepared in Example 1 and the savatiside A prepared in Example 2 were added with distilled water to form a 1 mg / mL sample solution, and then diluted to 100 μg / mL, 50 μg / mL, 25 μg / mL, 12.5 μg / mL, 6.25μg / mL and 3.125μg / mL and six concentrations are available for use. At the same time, 100 μg / mL vitamin E (VE) solution was used as a positive control.

[0089] Take 100 μL of the above-mentioned sample solutions of different concentrations in a 96-well ELISA plate, make three parallel wells for each concentration, then add 100 μL of 1 mM DPPH test solution, shake for 30 seconds, and measure the absorbance of each well at 37°C and 517nm wavelength (Ap). Simultaneously measure the blank absorbance value (Ac) of the sample solution without DPPH and the absorbance value (A max) of the sample solution with DPPH but no sample solution (...

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Abstract

The invention relates to the field of traditional Chinese medicine, and discloses application of phenethyl alcohol glycoside monomer compounds savatiside E and savatiside A in preparing a medicine for preventing and treating myocardial ischemia and hypoxia. According to the invention, the ability of the savatiside E and savatiside A in clearing oxygen radicals is measured by a DPPH method, and the result indicates that the oxygen radicals can be cleared in a dose-dependent mode. The H2O2 and Na2S2O3-caused myocardial cell damage model is adopted to detect the influence on survival rate of the H2O2 and Na2S2O3-caused rat myocardial cell oxidative damage, and the result indicates that the survival rate of myocardial cells can be improved in a dose-dependent mode to suppress peroxidation damage. Moreover, the in-vivo experiments also indicate that the savatiside E and savatiside A can reduce the area of the coronary artery ligation-caused rat myocardial infarction.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to the application of phenylethanoid glycoside monomer compounds, especially the application of phenylethanoid glycoside monomer compounds savatiside E and savatiside A in the preparation of drugs for preventing and treating myocardial hypoxic-ischemic diseases . Background technique [0002] Antler grass, English name: Antlerpilose grass, is the whole herb of Monochasma savatieri Franch of the genus Scrophulariaceae, with a plant height of 15-40 cm, covered with dense silver-white hairs. The stems are thin and hard, branched in clusters. Leaves opposite or three-lobed whorled, dense, internodes shortened; base leaves scale-like, 3-5 mm long; upper leaves narrowly lanceolate, 1-2 cm long, 1-3 mm wide, entire, Apex acuminate, base narrow, sessile. Flowers are solitary in the leaf axils, each flower has 2 linear bracteoles, inserted at the base of the calyx tube; the calyx is tube-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7034A61K31/7032A61P9/10A61P39/06A61P9/00
Inventor 刘艳丽许琼明何文君师梦凡陈重李笑然杨世林
Owner SUZHOU UNIV
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