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A kind of extraction and purification method of fucoidan sulfate ester

A technology of fucoidan sulfate and purification method, applied in the field of extracting and purifying fucoidan sulfate, can solve the problems of polysaccharide breakage, increased loss, affecting the extraction rate of polysaccharide, etc., so as to reduce the bulk density and improve the yield. rate, and the effect of reducing the total amount of solvent used

Inactive Publication Date: 2016-01-20
SHANDONG MEICHEN ECOLOGY & ENVIRONMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strong mechanical shearing effect of ultrasonic waves, long-term action will break the polysaccharides of macromolecules, thereby increasing the loss in the post-treatment process and affecting the extraction rate of polysaccharides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 200g of kelp waste residue, dry and pulverize, pass through an 80-mesh sieve to make a dry powder, add 400g of deionized water with a pH of 5 to the dry powder, and stir evenly. Microwave extraction at a microwave frequency of 2450MHz and a power of 800W for 2 minutes, and then add 400g of deionized water with a pH of 5, continued microwave extraction for 2 minutes under the same conditions, and extracted 4 times in total. After centrifuging the extract, take the first supernatant, concentrate the first supernatant to 800ml, add absolute ethanol to the supernatant while stirring to make the concentration reach 25%, and after the white precipitate precipitates, continue stirring for 20min. Centrifuge and take the second supernatant. Cool the second supernatant to 8 ° C, and slowly add absolute ethanol to make the concentration reach 70%, under vigorous stirring, the precipitate gradually precipitates, collect the precipitate,

[0023] After washing with absolute et...

Embodiment 2

[0025] Take 200g of kelp waste residue, dry and pulverize, pass through an 80-mesh sieve to make dry powder, add 600g of deionized water with a pH of 6 to the dry powder, and stir evenly, microwave for 6 minutes at a microwave operating frequency of 2450MHz and a power of 800W, and then add 600g of deionized water with a pH of 6, continued microwave extraction for 6 minutes under the same conditions, and extracted 3 times in total. After centrifuging the extract, take the first supernatant, concentrate the first supernatant to 800ml, and add absolute ethanol to the supernatant while stirring to make the concentration reach 30%. After the white precipitate is precipitated, continue stirring for 20min. Centrifuge and take the second supernatant. Cool the second supernatant to 5°C, and slowly add absolute ethanol to make the concentration reach 75%. Under vigorous stirring, the precipitate gradually precipitates, collects the precipitate, washes with absolute ethanol, and obtains...

Embodiment 3

[0027] Take 300g of dried kelp, dry and pulverize, pass through an 80-mesh sieve to make dry powder, add 800g of deionized water with a pH of 6 to the dry powder, and stir evenly, microwave for 6 minutes at a microwave operating frequency of 2450MHz and a power of 1000W, and then add 600g of deionized water with a pH of 6, continued microwave extraction for 6 minutes under the same conditions, and extracted 4 times in total. After centrifuging the extract, take the first supernatant, concentrate the first supernatant to 1200ml, add absolute ethanol to the supernatant while stirring to make the concentration reach 30%, and after the white precipitate precipitates, continue stirring for 20min. Centrifuge and take the second supernatant. Cool the second supernatant to 5°C, and slowly add absolute ethanol to make the concentration reach 75%. Under vigorous stirring, the precipitate gradually precipitates, collects the precipitate, washes with absolute ethanol, and obtains fine par...

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PUM

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Abstract

The invention relates to an extracting and purifying method of fucoidan from fucus vesiculosus. The extracting and purifying method comprises the following steps of: S1, with sea-tangle waste generated after mannitol is extracted as raw material, adding deionized water with pH of 5-6 according to a proportion, intermittently extracting for 3-6 times under the microwave condition of 2450MHz and 600-1200W, wherein each extraction is carried out for 2-7min and a certain proportion of deionized water is replenished every time; S2, centrifuging an extraction solution to obtain supernate, concentrating the supernate and then adding a certain proportion of alcohol, generating a sediment, centrifuging again to obtain supernate; S3, cooling the supernate to 5-10DEG C, slowly adding a certain proportion of alcohol, seriously stirring, gradually generating a white particle sediment; and S4, collecting the sediments, washing by using absolute ethyl alcohol, and drying to obtain the fine particle fucoidan from fucus vesiculosus.

Description

technical field [0001] The invention relates to a method for extracting and purifying fucoidan sulfate from kelp waste residue by using microwaves. Background technique [0002] Fucoidan sulfate, also known as fucoidan and fucoidan sulfate, is an intercellular polysaccharide inherent in brown algae such as kelp. It exists in the cell wall matrix of brown algae and is a unique biologically active substance of brown algae. Fucoidan sulfate in kelp is a water-soluble heteroglycan. Its main component is L-fucose-4-sulfate, and a small amount of galactose, mannose, xylose, glucose, arabinose, uronic acid, protein and potassium, sodium, calcium, magnesium and other metal ions. The structure of L-fucose-4-sulfate is characterized by 1,2-linked poly a-L-fucopyranose. The main chain is composed of fucose, which contains a large number of sulfate groups, which is their common feature. Moreover, fucoidan sulfate has various physiological activities such as anticoagulation, lowering ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 李红芬梁军军刘云
Owner SHANDONG MEICHEN ECOLOGY & ENVIRONMENT CO LTD