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Novel creating method for processing serial high-quality protein into special soybean germplasm

A high-quality protein, special-purpose technology, applied in the fields of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of inability to achieve large-scale preparation or production, and high equipment requirements

Inactive Publication Date: 2013-02-27
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

At present, Zheng et al. (2009) of South China University of Technology used anion exchange chromatography and immobilized metal ion affinity chromatography (IMAC) to achieve the purpose of obtaining high-purity β-conglycinin. It has high requirements on equipment and cannot realize mass preparation or production

Method used

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Experimental program
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Effect test

Embodiment 1

[0063] A new method for the creation and application of a series of special soybean germplasm for high-quality protein processing, the specific operation is as follows:

[0064] 1. Screening of germplasm lacking major storage protein subunits in soybean seeds

[0065] Test germplasm: 1400 soybean germplasm resources collected, including wild soybeans, local varieties, and popular varieties.

[0066] From 2001 to 2003, SDS-PAGE method was used to identify and screen out 1400 soybean germplasm resources collected to identify Wuhe soybean (α′ deletion), Liuyang Wuyuehuang (α deletion) and Suhuipod (β deletion) , Jiyu 32 (A 1a B 2 missing), Xingwen black beans (A 2 B 1a missing), Guiyang Zijindou (A 1b B 1b missing), 493-1(A 5 A 4 B 3 Missing), Anning 1 (A 3 B 4 Deletion) and other protein subunit deletion material. And after 2 years of planting observation, it shows that the inheritance of these germplasm protein subunit deletion genes is stable.

[0067] 2. Polymeri...

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Abstract

The invention discloses a novel creating method for processing serial high-quality protein into special soybean germplasm. Chinese soybean germplasm resources have rich main seed storage protein (7S and 11S components) subunit deleted genes, the genes are identified and selected by means of pyramiding breeding and cross breeding and by an SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) method, 7S component protein subunit deleted germplasm, 11S component protein subunit deleted germplasm and 7S+11S component protein subunit all-deleted germplasm are firstly created, the specific germplasm is further respectively crossly combined with current Chinese major varieties, various 7S and 11S component protein subunit single-deleted, double-deleted, triple-deleted, quadruple-deleted, quintuple-deleted, sextuple-deleted, septuple-deleted and all-deleted materials with genetic stability are finally obtained by means of selection and stabilization and are further cultivated into strains, accordingly, the cultivating process of Chinese protein processed special soybean varieties is accelerated, and high-quality raw materials are provided for Chinese soybean protein processing and utilization.

Description

technical field [0001] The invention belongs to the fields of crop genetic breeding, quality improvement and utilization, and relates to a new method for creating a series of special soybean germplasm for high-quality protein processing. Background technique [0002] Soybean seed protein and oil content account for about 40% and 20% of the dry weight of the seed, respectively. The utilization of soybean seed protein is not only the use of its nutrition, such as it contains 8 kinds of amino acids necessary for the human body, it has functions such as anti-tumor, enhancing immunity, and preventing cardiovascular diseases, but more importantly, it uses its processing characteristics-functionality, Such as gelling, emulsifying, foaming, oil holding, water holding, etc. to improve the texture and nutrition of food. The latter is especially important in the food processing industry. For example, meat products need to use the oil-holding and water-holding properties of soybean pro...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H5/10G01N27/447
Inventor 麻浩何小玲刘春张国敏杨艳
Owner NANJING AGRICULTURAL UNIVERSITY
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