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Recombinant Newcastle disease LaSota attenuated vaccine strain expressing mycoplasma gallisepticum TM1protein

A technology of mycoplasma gallisepticum and attenuated vaccines, which is applied in the field of recombinant virus vaccines and can solve the problems of large antigenic differences of strains and difficulties in MG cultivation

Inactive Publication Date: 2013-03-13
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, it is difficult to cultivate MG, and the antigenicity of strains isolated from different regions is quite different. Conventional vaccines have been unable to prevent and treat MG. Therefore, it is imminent to develop new vaccines by using advanced technology, and the development of MG genetically engineered vaccines has been in its infancy.

Method used

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  • Recombinant Newcastle disease LaSota attenuated vaccine strain expressing mycoplasma gallisepticum TM1protein
  • Recombinant Newcastle disease LaSota attenuated vaccine strain expressing mycoplasma gallisepticum TM1protein
  • Recombinant Newcastle disease LaSota attenuated vaccine strain expressing mycoplasma gallisepticum TM1protein

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Experimental program
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Effect test

Embodiment 1

[0039] Example 1 Construction and biological activity of recombinant Newcastle disease LaSota attenuated vaccine expressing the gene encoding Mycoplasma gallisepticum TM1 protein

[0040] 1 Materials and methods

[0041] 1.1 Cells, viruses, serum, chicken embryos and experimental animals

[0042] Vero-E6 cells (African green monkey kidney cells ATCC No.CRL-1586), BHK-21 cells (suckling hamster kidney cells ATCCCCL-10) and CEF cells (chicken embryo fibroblasts) were purchased from Harbin Veterinary Institute Zoonosis Preserved in the research laboratory; the MG strain used for the attack was donated by Shao Guoqing, a researcher of the Jiangsu Academy of Agricultural Sciences; the recombinant Newcastle disease virus rLaSota is a recombinant LaSota virus that does not contain foreign genes rescued by reverse genetic manipulation technology, which was developed by the inventor according to ZL200510097997. 8 Prepared and preserved; recombinant poxvirus vTF7-3 (ATCC, VR-2153) stab...

Embodiment 2

[0071] Embodiment 2 Recombinant virus immunoassay and serum antibody detection

[0072] 1. Materials and methods

[0073] 1.1 Materials

[0074] With above embodiment 1.

[0075] 1.2 Method

[0076] Dilute the recombinant virus stock solution to 10 6 EID 50 Twenty 3-day-old SPF chicks were vaccinated in doses, and the vaccinated SPF chicks were reared in a negative pressure isolator. Wing venous blood was collected at intervals of one week, serum was separated, and anti-NDV HI antibodies and MG-specific ELISA antibodies were detected. The LaSota inoculated group was used as a control.

[0077] Three weeks after the immunization, take the virulent strain of MG and inoculate the virus through the tracheal route, and each chicken is inoculated with 0.5ml. Observe the respiratory symptoms of the chicks, and collect wing vein blood after 14 days, then carry out autopsy, observe the air sac lesions, and carry out scoring records.

[0078] 2 results

[0079] Dilute the recomb...

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Abstract

The invention relates to a recombinant Newcastle disease LaSota attenuated vaccine strain expressing a mycoplasma gallisepticum TM1protein, more particularly, the recombinant Newcastle disease LaSota attenuated vaccine is rLa-tPA-TM1. The invention also discloses a method for preparing the recombinant Newcastle disease LaSota attenuated vaccine and application of the recombinant Newcastle disease LaSota attenuated vaccine in the preparation of bivalent vaccines for controlling diseases caused by mycoplasma gallisepticum and Newcastle disease.

Description

technical field [0001] The invention relates to the field of recombinant virus vaccines. More specifically, the invention relates to a recombinant Newcastle disease LaSota attenuated vaccine expressing Mycoplasma gallisepticum TM1 protein. More specifically, the recombinant Newcastle disease LaSota attenuated vaccine is rLa-tPA-TM1. The invention also discloses a method for preparing the recombinant Newcastle disease LaSota attenuated vaccine and the application of the recombinant Newcastle disease LaSota attenuated vaccine in preparing bivalent vaccines for preventing diseases caused by mycoplasma gallisepticum and Newcastle disease. Background technique [0002] Mycoplasma gallisepticum (MG) can cause chronic respiratory disease, air sacculitis and sinusitis in infected chickens, and is one of the common diseases affecting poultry production. Affected chickens often show dyspnea, cough and rales, and infraorbital sinusitis and air sac inflammation are common. The disease ...

Claims

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Application Information

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IPC IPC(8): A61K39/295C12N15/45A61P31/04A61P31/14A61K39/02A61K39/17
Inventor 步志高陈化兰葛金英邵国青
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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