Composite microbial bactericide, and preparation method and application thereof

A technology of compound microorganisms and compound inoculants, applied in the field of compound microorganism fungicides and their preparation, can solve the problems of single efficacy and enhanced resistance of pathogenic bacteria, and achieve the effects of strong control ability, long lasting effect and strong colonization ability.

Active Publication Date: 2014-05-14
湖南慧谷农业生态研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the shortcomings of existing chemical fungicides with single efficacy and long-term application that lead to increased resistance to pathogenic bacteria, the present invention provides a composite microbial fungicide, which is prepared by raw materials including the following components in parts by weight: to make:

Method used

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  • Composite microbial bactericide, and preparation method and application thereof
  • Composite microbial bactericide, and preparation method and application thereof
  • Composite microbial bactericide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1 Composite microbial bactericide

[0064] The composition of composite microbial bactericide described in the present embodiment:

[0065] 10kg of compound bacteria agent 10kg of nutrients; 2kg of enzyme active substances; 78kg of fungicide carrier.

[0066] The above compound microbial agent contains: Trichoderma harzianum 4.5×10 8 cfu / g; Trichoderma viride 2×10 8 cfu / g; Bacillus subtilis 0.5×10 8 cfu / g; Bacillus licheniformis 1×10 8 cfu / g; total effective bacteria content ≥8×10 8 cfu / g.

[0067] Wherein, the above-mentioned composite microbial agent is prepared by the following steps:

[0068] Preparation of bacterial mixed spore powder:

[0069] ①Slant culture: Inoculate the original strains of Bacillus subtilis and Bacillus licheniformis on the slant medium under sterile conditions, and cultivate them at 29±1°C for 48 hours;

[0070] ②Shaking table culture: inoculate the strains cultured in the above step ① into the liquid medium under sterile co...

Embodiment 2

[0092] Embodiment 2 Composition of composite microbial bactericide

[0093] Compared with Example 1, the difference is only that the composition of the compound microbial bactericide described in this example is:

[0094] Compound bacterial agent 20kg; nutritional ingredients 20kg; enzyme active substance 4kg; fungicide carrier 56kg.

[0095] The composition of the above-mentioned composite bacterial agent:

[0096] Trichoderma harzianum 7.5×10 8 cfu / g; Trichoderma viride 4×10 8 cfu / g; Bacillus subtilis 1.5×10 8 100 million cfu / g; Bacillus licheniformis 3×10 8 Billion cfu / g; total effective bacteria content ≥12×10 8 cfu / g.

[0097] Wherein, the above-mentioned composite microbial agent is prepared by the following steps:

[0098] Preparation of bacterial mixed spore powder:

[0099] ① Slant culture: Inoculate the original strains of Bacillus subtilis and Bacillus licheniformis on the slant medium under sterile conditions, and culture at 29±1°C for 36 hours;

[0100] ②...

Embodiment 3

[0112] Compared with Example 1, the difference is only that the composition of the compound microbial bactericide described in this example is:

[0113] Compound bacterial agent 15kg; nutritional ingredients 15kg; enzyme active substance 4kg; fungicide carrier 66kg.

[0114] The composition of the above-mentioned composite bacterial agent:

[0115] Trichoderma harzianum 6×10 8 cfu / g; Trichoderma viride 3×10 8 cfu / g; Bacillus subtilis 1×10 8 100 million cfu / g; Bacillus licheniformis 2×10 8 Billion cfu / g; total effective bacteria content ≥12×10 8 cfu / g.

[0116] Wherein, the above-mentioned composite microbial agent is prepared by the following steps:

[0117] Preparation of bacterial mixed spore powder:

[0118] ① Slant culture: Inoculate the original strains of Bacillus subtilis and Bacillus licheniformis on the slant medium under sterile conditions, and culture at 29±1°C for 36 hours;

[0119] ② Shaking table culture: Inoculate the strains cultured in the above step ①...

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Abstract

The invention provides a composite microbial bactericide, and a preparation method and application thereof. The composite microbial bactericide is composed of the following components in parts by weight: 10-20 parts of composite microbial inoculum, 10-20 parts of nutritional ingredient, 4-6 parts of enzyme activated substance and 54-78 parts of bactericide carrier, wherein the composite microbial inoculum contains biocontrol growth-promoting bacteria and a protein-decomposing bacterium; the biocontrol growth-promoting bacteria include Trichoderma harzianum, Trichoderma viride and Bacillus licheniformis; and the protein-decomposing bacterium is Bacillus subtilis. The invention also provides a preparation method of the composite microbial bactericide. The composite microbial bactericide provided by the invention can be colonized and grow quickly at the rhizosphere of a plant and form a protecting cover, thus preventing the infection of pathogenic fungi at the root; antibiotics and other plant growth-promoting substances are generated in the pathogenic bacterium interaction and hyperparasitization processes; multiple diseases of crops can be remarkably prevented and antagonized or be directly killed; and meanwhile, the composite microbial bactericide has a root promotion and germination accelerating effect and can promote the healthy growth of the crops, thereby improving the yield and the quality.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms, and in particular relates to a compound microbial bactericide and its preparation method and application. Background technique [0002] At present, in agricultural production, with the long-term continuous cropping of crops, soil pathogens have accumulated for a long time, and soil-borne diseases have occurred seriously. In addition, the improper use of chemical pesticides and fertilizers has led to serious soil compaction and salinization, and the output of agricultural products has dropped sharply, or even failed. , poor quality and low price, which have a great impact on farmers. Therefore, how to develop and correctly use biological fungicides is the first choice for the development of organic and green agriculture. [0003] The prior art discloses a variety of microbial bactericides, such as: [0004] Chinese application 200910016337.0 discloses a Trichoderma harzianu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P3/00C05G3/00
Inventor 曹典军唐八生彭群林炜郭帅蔡浩
Owner 湖南慧谷农业生态研究院有限公司
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