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Method for screening anti-bacterial adhesion inhibitors and preparation of anti-adhesion oral liquid

A technology for anti-bacterial adhesion and oral liquid, which is applied in the direction of antibacterial drugs, medical preparations containing active ingredients, and microbial measurement/inspection, and can solve the problems of false negatives of anti-bacterial adhesion inhibitors, etc.

Inactive Publication Date: 2014-05-28
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Another object of the present invention is: to overcome the shortcomings that traditional methods may cause false negatives when screening anti-bacterial adhesion inhibitors, the present invention provides a method that can visually judge whether the sample to be screened has anti-bacterial adhesion

Method used

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  • Method for screening anti-bacterial adhesion inhibitors and preparation of anti-adhesion oral liquid
  • Method for screening anti-bacterial adhesion inhibitors and preparation of anti-adhesion oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of antibacterial adhesion oral liquid of the present invention

[0024] 1. Materials and methods

[0025] (1) Materials: The rhizomes of Lengshuihua were collected from Thunder Rock Canyon, Guiyang City, the honeysuckle was collected from Lover Valley, Guiyang City, and the poria cocos was purchased from Jiuchao Township, Liping County, Guizhou Province. The rhizomes of Lengshuihua are chopped and dried in the shade, the honeysuckle is dried in the shade, the poria cocos is peeled and cut into cubes or slices, and dried in the shade for later use. Kombucha was purchased from the Kombucha Cultivation and Research Center in Yueyang City, Hunan Province.

[0026] (2) Method: Dried 400 grams of the neck root and 600 grams of honeysuckle in cold water, added 6 liters of water and decocted three times to obtain 4 liters of the filtrate, concentrated to obtain the extract, put the extract in a water bath and evaporated to dryness, and obta...

Embodiment 2

[0033] Embodiment 2: Anti-bacterial adhesion oral liquid of the present invention anti-bacterial infection animal test

[0034] 1. Materials and methods

[0035] (1) Materials: Escherichia coli U07, Salmonella enteritidis ATCC 13076, Kunming mice, New Zealand white rabbits.

[0036] (2) method

[0037] a. Mice artificially infected with Escherichia coli and Salmonella enteritidis and treatment experiment. Kunming mice were purchased from the Animal Center of Guiyang Medical College. Mice were divided into infection model group (water supply), anti-bacterial adhesion oral liquid group of the present invention (three measurements, 0.1, 0.3, 0.6 ml / mouse), amoxicillin group (10 mg / mouse), kombucha liquid group (0.6 ml / mouse), cold Jinling aqueous solution group (that is, without kombucha fermentation) (0.6 ml / mouse), 12 mice in each group, half male and half male. After the mice were fasted for 12 hours, 0.2ml (1×10 8 CFU / ml) bacterial suspension (blunt needle ins...

Embodiment 3

[0047] Embodiment 3: Determination of the antibacterial adhesion effect of medicine based on culture fluid slide immersion method

[0048] 1. Materials and methods

[0049] (1) Experimental materials: ordinary broth, rifamycin, anti-bacterial adhesion oral solution of the present invention, Escherichia coli U07 strain (isolated from a clinical patient with urinary tract infection).

[0050] (2) Experimental method

[0051] (a) Crystal violet chromogenic method (traditional method): Rifamycin is used as a positive control drug. Rifamycin is first dissolved in a small amount of DMSO (dimethyl sulfoxide), and then diluted with ordinary broth (the final concentration of DMSO is less than 0.5%) into 2, 4, 8 solution (the minimum inhibitory concentration (MIC) of rifamycin against Escherichia coli U07 strain measured before the experiment was 16 ). The anti-bacterial adhesion oral liquid of the present invention is diluted into 0.5, 1, 2% (V / V) solutions with common bro...

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Abstract

The invention discloses a novel method for screening anti-bacterial adhesion inhibitors and preparation of anti-bacterial adhesion oral liquid. The novel method disclosed by the invention comprises the following steps of: immersing a glass slide with a specific shape into a liquid culture medium to carry out bacterial adhesion; adding a screened sample into the culture medium; and analyzing and judging anti-bacterial adhesion medicinal effect through a microscopic image. The anti-bacterial adhesion oral liquid disclosed by the invention is prepared from the raw materials including pilea notata, honeysuckle and poria cocos through extracting, liquid matching, fermenting, freezing and centrifugal processes and has the pharmaceutical effects of resisting bacterial adhesion, intestinal infection, urinary tract infection and cold.

Description

technical field [0001] The invention relates to a new method for screening anti-bacterial adhesion inhibitors and preparation of an anti-bacterial adhesion oral liquid. Background technique [0002] Antibiotics have made great contributions to the treatment and prevention of bacterial infections, but due to the widespread use (including misuse) of antibiotics, the problem of bacterial resistance has become a major problem that seriously threatens human health. The characteristic of existing antibacterial drugs is to destroy the life function of bacteria, such as interfering with the synthesis of bacterial cell walls, disrupting the replication of bacterial DNA or protein synthesis, etc. It is the selection pressure of these mechanisms of action that promotes the emergence of drug-resistant strains. In order to solve the problem of bacterial drug resistance, a new idea of ​​antibacterial drug research and development has gradually formed, that is, to study new antibacterial ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/35A61K9/08A61P31/04C12Q1/02
Inventor 杨再昌杜润孜陆伦维杨小生
Owner GUIZHOU UNIV
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