Water-soluble culture medium for edible fungus liquefaction strain

A water-soluble edible fungus technology, applied in the field of culture medium, can solve the problems of difficult transportation of liquid strains, long growth cycle of strains, inconvenient operation, etc., and achieve neat fruiting, fast growth speed, and shortened fruiting time Effect

Inactive Publication Date: 2013-03-20
TIANSHUI ZHONGXING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The production of edible fungus strains in the prior art mainly contains two production methods of solid strains and liquid strains. The production of solid strains has the disadvantages of long growth cycle of the strains, inconsistent bacterial age, inconvenient operation, and high cost. Liquid strains have disadvantages such as difficult transportation, storage, testing, miscellaneous removal, and high investment costs, which have become the main factors restricting the development of industrialized edible fungi.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0011] The preparation method of the liquefied strain of Flammulina velutipes comprises the following steps:

[0012] Step 1: Purification and cultivation of primary strains

[0013] (1) Preparation of PDA-enriched medium: remove 300g of potatoes, peel them, cut them into small strips, put them in a pot, add 1500ml of water, boil until the potatoes are crispy and not rotten, filter them with 6 layers of gauze to obtain PDA culture base; add 30g of agar to melt, then add 30g of glucose, 1.0g of plant-based peptone, and 3.0g of dry yeast flakes, mix and stir evenly, add water to 1500ml to obtain PDA-enriched medium, and distribute it in 20mm×200mm test tubes while hot; Plug the test tubes with cotton plugs, wrap each of the 7 tubes with kraft paper, and fix them with rubber bands for later use.

[0014] (2) Sterilization and cooling of PDA enriched medium: Put the wrapped test tube into a high-pressure steam sterilizer with a pressure of 1.5kg / m 3 , the temperature was 121°C, ...

Embodiment 2

[0028] The preparation method of the liquefied strain of Pleurotus eryngii comprises the following steps:

[0029] Step 1: Purification and cultivation of primary strains

[0030] (1) Preparation of PDA-enriched medium: remove 300g of potatoes, peel them, cut them into small strips, put them in a pot, add 1500ml of water, boil until the potatoes are crispy and not rotten, filter them with 6 layers of gauze to obtain PDA culture base; add 20g of agar to melt, then add 40g of glucose, 1.2g of peptone, and 2.5g of yeast flakes, mix and stir evenly, replenish water to 1500ml to obtain PDA-enriched medium, and divide it into 20mm×200mm test tubes while hot; plug the test tube Put on tampons, wrap each of 7 with kraft paper, and fix them with rubber bands for later use.

[0031] (2) Sterilization and cooling of PDA enriched medium: Put the wrapped test tube into a high-pressure steam sterilizer with a pressure of 1.4kg / m 3 , the temperature was 122°C, and heated for 30 minutes to ...

Embodiment 3

[0045] The preparation method of the liquefied strain of crab-flavored mushroom comprises the following steps:

[0046] Step 1: Purification and cultivation of primary strains

[0047] (1) Preparation of PDA-enriched medium: remove 300g of potatoes, peel them, cut them into small strips, put them in a pot, add 1200ml of water, boil until the potatoes are crispy and not rotten, filter them with 5 layers of gauze to obtain PDA culture base; add 25g of agar to melt, then add 20g of glucose, 1.5g of peptone, and 2.0g of yeast flakes, mix and stir evenly, replenish water to 1200ml to obtain PDA enriched medium, and divide it into 20mm×200mm test tubes while hot; plug the test tube Put on tampons, wrap each of 7 with kraft paper, and fix them with rubber bands for later use.

[0048] (2) Sterilization and cooling of PDA enriched medium: Put the wrapped test tube into a high-pressure steam sterilizer with a pressure of 1.4kg / m 3 , the temperature was 123°C, and heated for 35 minute...

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Abstract

The invention discloses a water-soluble culture medium for an edible fungus liquefaction strain, which is prepared from the following raw materials in parts by mass: 150-170 parts of white sugar, 17-19 parts of bean pulp, 26-30 parts of magnesium sulfate, 26-30 parts of potassium dihydrogen phosphate, 1-3 parts of defoaming agent and 550-580 parts of sterilized water by evenly mixing and stirring at 17-19 DEG C. The water-soluble culture medium for an edible fungus liquefaction strain is simple to prepare; and after an edible fungus liquefaction strain prepared from the culture liquid is inoculated, the strain has high permeability and high growth speed. Besides, through the culture of the edible fungus liquefaction strain, the fruiting time is greatly shortened, fruits grow regularly and quickly, and the yield is obviously increased.

Description

technical field [0001] The invention relates to a culture medium, in particular to a water-soluble culture medium for edible mushroom liquefaction strains. Background technique [0002] In the production of edible fungi, the key to success and failure is the production of strains. The largest investment in production is the strains that people are most worried about. The purity, vigor, cultivation time and anti-impurity ability of the strain determine the success or failure of production, the level of benefit and the quality of the product. The production of edible fungus strains is a key link in the cultivation of edible fungi. To achieve high-quality and high-efficiency edible fungus cultivation, the high-quality, high-efficiency and low-cost of the strains must first be achieved. [0003] The production of edible fungus strains in the prior art mainly contains two production methods of solid strains and liquid strains. The production of solid strains has the disadvantage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 陶军
Owner TIANSHUI ZHONGXING BIO TECH
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