Method for producing L-tert-leucine

Abstract

The invention relates to the fields of biological pharmacy and biochemical engineering, and in particular relates to a method for producing L-tert-leucine. The method comprises the step of synthesizing L-tert-leucine through the L-tert-leucine dehydrogenase and formate dehydrogenase enzymic method, wherein in the enzyme catalysis step, the enzyme feeding amount is 4 percent (w / w) of a substrate, the coenzyme feeding amount is 0.2 percent (w / w) of the substrate, the space time yield is 100g / d / L, the product purity is more than 98 percent, and the optical purity is more than 99 percent. Compared with the traditional preparation process, the method has the advantages that the catalyst enzyme amount is effectively reduced, the product is high in purity and high in yield, the process flow is simple, special equipment requirements are avoided, the method is suitable for industrial production, and the product can be widely applied to chemical intermediates and medical intermediates.

Description

technical field [0001] The invention is applicable to the technical fields of biopharmaceutical and biochemical industry, and in particular relates to a production method of L-tert-leucine. Background technique [0002] Unnatural amino acids and their derivatives are important chemical intermediates. Among them, L-tert-leucine is an important unnatural amino acid. It can be used in the synthesis of pharmaceutical intermediates, as a chiral inducer for asymmetric synthesis and as a resolution reagent for asymmetric resolution, and as a chiral precursor for the synthesis of various anti-AIDS drugs and hepatitis virus inhibitors. With the development of my country's social economy and the enhancement of environmental protection awareness, the pharmaceutical and chemical industry not only needs to increase the production capacity of L-tert-leucine, but also needs to create a green and pollution-free production process. Therefore, the use of enzymatic biotransformation to produ...

AI Technical Summary

Problems solved by technology

For example, in the patent CN201110116253.1, the cost of the expensive coenzyme NAD is 1% (w / w) of the substrate, which is economical. In the patent CN201110277030.3, the concentration of the whole cell catalyst in the reaction system is 80% (4g / 5 mL) ; 3. No definite post-extraction process

Also due to the existence of problem 1, its yield and yield cannot meet the needs of industrialized production

Based on the above three points, there is no report on the large-scale production of L-tertiary leucine using this method