35 results about "L-tert-leucine" patented technology

The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals. A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.

The invention relates to a method for preparing a chiral compound based on splitting by adopting a biological enzyme preparation, in particular to a method for preparing an L-tertiary leucine compound by a two enzyme system. The method prepares the chiral L-tertiary leucine compound of the formula III by using the reaction of an N-acylating tertiary leucine compound of the formula II in water or a buffer solvent at 15 to 60 DEG C in the presence of the two enzyme system. The biological enzyme is a mixed enzyme of an acylating amino acid racemase (alanine racemase) and a hydrolase; and the preparation reaction equation is as follows, wherein X is any group of OH, NH2, NR1R2 and OR3 (wherein R1, R2, R3 are a C1-C5 straight chain, a branch chain and a one, two or three-halogen substituted alkane respectively). The invention can be implemented at room temperature and ensures simple operation and no pollution, while remarkably lowering the production cost.

The invention discloses a novel method for synthesizing Atazanavir. The method comprises steps of: carrying out a nucleophilic substitution reaction on a compound shown as a formula I N-1-(t-butoxycarbony)-N-2-[4-(2-pyridyl) benzylidene]-hydrazine and a compound shown as a formula II (S)-4-chlorine-3-carbonyl-1-phenyl butane-2-tert-butyl carbamate to obtain an intermediate shown as a formula III; removing protective groups of the intermediate shown as a formula III and carrying out condensation reaction with N-methoxycarbonyl-L-tert-leucine to obtain an intermediate shown as a formula IV; carrying out a reduction reaction on the intermediate shown as the formula IV; and a reaction formula is shown as below. The synthetic method has advantages of mild reaction conditions, high security, simple operation, simple purification treatment on end product, high purity, stable quality, easily available raw material, low price and high overall yield, so as to substantially reduce cost and apply to demand of large-scale industrialized production.

The invention relates to a new method for preparing telaprevir, which comprises the following steps: 1) orderly coupling the following 5 substances according to a Fmoc solid-phase synthetic method with a resin as a carrier to obtain peptide resin: -amino acid Fmoc-P-OH, -Fmoc-octahydrocyclopenta[c]pyrrole-1-carboxylic acid, -Fmoc-L-tertiary leucine, -Fmoc-2-cyclohexyl glycine, and -2-pyrazinecarboxylic acid; 2) cracking the peptide resin to obtain deprotected peptide; 3) allowing the deprotected peptide to react with cyclopropylamine under a liquid-phase condition to obtain the telaprevir; wherein the Fmoc is 9-fluorenylmethyloxycarbonyl, and is connected with an amino, or with a nitrogen atom on a carbon atom connected with a carboxyl. The method for preparing telaprevir of the invention is simple in operation, low in cost, and high in yield.

The invention discloses a method for preparing highly pure L-tertiary leucine through a biosynthesis process. The method comprises the following steps: inoculating an Escherichia coli seed liquid expressing leucine dehydrogenase gene and formic dehydrogenase gene into in a fermenting culture medium of a self-induction system, carrying out fermenting culture, centrifuging to obtain crude thalli, and adding a buffer solution to obtain a cell suspension; adding substrates comprising trimethylpyruvic acid and ammonium formate, and carrying out a biological catalysis reaction to obtain an trimethylpyruvic acid conversion solution; and centrifuging the conversion solution, adjusting the pH value of the solution, carrying out column chromatography, precipitating, and concentrating to obtain highly pure L-tertiary leucine. The method fully uses the high effectiveness, the specificity and the mildness of an enzyme; and compared with traditional chemical synthesis methods, the method disclosed in the invention has the advantages of high selectivity, mild conditions, simple process, low cost, small pollution and the like.

The invention discloses a method for simultaneously preparing D-tert-leucine and L-tert-leucine. The method comprises the following steps: 1, reacting n-butanol with 1,1-dichloroethylene to obtain a 3,3-dimethylbutyric acid product; 2, adding a catalyst to the 3,3-dimethylbutyric acid product, introducing air and chlorine, and carrying out a chlorination reaction to obtain 2-chloro-3,3-dimethylbutyric acid; 3, carrying out an aminolysis reaction on 2-chloro-3,3-dimethylbutyric acid to obtain D, L-tert-leucine; 4, carrying out acetylation and chloroacetylation on the obtained D, L-tert-leucine, and using L-thermal stability amino acid acylase splitting and other treatments to respectively obtain D-tert-leucine and L-tert-leucine; and 5, racemizing acetylated and chloroacetylated D-tert-leucine recovered after splitting, and carrying out cycle splitting. N-butanol and 1,1-dichloroethylene with low cost are used as initial raw materials. The cost of the method is substantially lower than that of a biological reduction method.

The invention discloses a method for preparing L-tertiary leucine based on biological brick tandem double enzymes. The method comprises the following steps: (1) constructing a tandem biological brick element capable of realizing tandem expression of leucine dehydrogenase and formate dehydrogenase; (2) introducing the tandem biological brick element into Escherichia coli (E.coli) so as to construct E.coli engineering bacteria based on tandem expression of leucine dehydrogenase and formate dehydrogenase; (3) inoculating the E.coli engineering bacteria into a liquid enlarged culture medium containing chloromycetin for carrying out culture and induced expression to obtain fermentation liquid, carrying out refrigerated centrifugation to obtain cells, and re-suspending and washing with a buffer solution so as to obtain cell sap; and (4) putting the cell sap, trimethylpyruvic acid, amino donors, coenzymes and cosubstrates for regeneration of the coenzymes in a buffer system for carrying out vibration reaction, and carrying out whole-cell catalysis asymmetric reductive amination to obtain the product, namely L-tertiary leucine. The method disclosed by the invention has the advantages that the product conversion rate is high, the chiral selectivity is better, the reaction conditions are mild, the operation is simple, the expensive coenzymes can be regenerated, and the cost is saved by expressing the double enzymes with single cells.

The invention relates to the fields of biological pharmacy and biochemical engineering, and in particular relates to a method for producing L-tert-leucine. The method comprises the step of synthesizing L-tert-leucine through the L-tert-leucine dehydrogenase and formate dehydrogenase enzymic method, wherein in the enzyme catalysis step, the enzyme feeding amount is 4 percent (w/w) of a substrate, the coenzyme feeding amount is 0.2 percent (w/w) of the substrate, the space time yield is 100g/d/L, the product purity is more than 98 percent, and the optical purity is more than 99 percent. Compared with the traditional preparation process, the method has the advantages that the catalyst enzyme amount is effectively reduced, the product is high in purity and high in yield, the process flow is simple, special equipment requirements are avoided, the method is suitable for industrial production, and the product can be widely applied to chemical intermediates and medical intermediates.

The invention discloses a method for preparing L-tert-leucine by coupling leucine dehydrogenase with glucose dehydrogenase, belonging to the technical field of enzyme engineering and chiral medical intermediate preparation. The method comprises the following steps: adding substrates trimethylpyruvic acid and glucose into a reaction system; adjusting the pH value of the system to 8.0-9.0; and adding a crude enzyme (containing leucine dehydrogenase, glucose dehydrogenase and coenzyme) with broken co-expressed strain and cultured by a culture medium added with substances such as niacin, and then starting reactions while controlling the temperature at 25-30 DEG C and pH value at 8.0-9.0 in the reaction process to generate L-tert-leucine. In the invention, a system in which leucine dehydrogenase is coupled with glucose dehydrogenase is adopted, and the L-tert-leucine is prepared by use of the coenzyme contained in the thalli; and the method has the characteristics of high concentration of single-batch reaction substrates, high reaction efficiency, no coenzyme addition and the like.

The invention discloses a method for preparing an atazanavir monomer. The method is applied to the technical field of medicines and comprises steps as follows: at the proper temperature, weak base is used as an acid-binding agent, under a certain organic solvent condition, N-methoxycarbonyl-L-tert-leucine firstly reacts with thionyl chloride to produce N-methoxycarbonyl-L-tert-leucine acyl chloride, then the N-methoxycarbonyl-L-tert-leucine acyl chloride and 1-[4-(pyridin-2-yl)-phenyl]-4(S)-hydroxyl-5(S)-2,5-diamino-6-phenyl-2-azahexane have an amide formation reaction at the room temperature, and the atazanavir monomer is obtained. The method has the advantages as follows: (1), the thionyl chloride is cheap, so that the costs of raw materials are effectively reduced, and the method is suitable for industrialization; (2), the produced pollution is less and is converted into soluble effluent brine after after-treatment, so that the method is relatively environment-friendly; (3), the operation is simple, the product yield is high, separation and purification are easy, and the method is suitable for industrial production.

Process for producing N-[(2S)-sulfanyl-4-(1,5,5-trimethylhydantoinyl) butanoyl]-L-leucyl-L-tert-leucine N-methylamide by forming an intermediate compound N-[(2S)-thiobenzoyl-4-(1,5,5-trimethylhydantoinyl)butanoyl]-L-leucyl-L-tert-leucine N-methylamide by an improved process that substantially reduces the presence of contaminants in the reaction mixture.

The invention relates to a preparation method of MOC (methoxycarbonyl group)-L-tertiary leucine, in particular to a method for preparing the MOC-L-tertiary leucine by separating 2-methoxyamide-3, 3-methylbutanoic acid. The method comprises the following steps of forming two kinds of diastereoisomer amine salts by using the 2-methoxyamide-3, 3-methylbutanoic acid and a resolving agent which is an amine compound; separating the two kinds of diastereoisomer amine salts to obtain MOC-L- tertiary leucine amine salt; and performing acid dissociation on the MOC-L- tertiary leucine amine salt to obtain the MOC-L-tertiary leucine. The method is low in cost, a technology is simple and reasonable, the purity of products is high, and the method is suitable for industrial production of the MOC-L-tertiary leucine.

The invention discloses a new method for preparing L-tertiary leucine. The new method comprises the following steps: 1, performing asymmetric cyanosilylation reaction on pivalaldehyde, L-phenylglycinol and trimethylsilyl cyanide in the presence of a catalyst A, and carrying out posttreatment to obtain a compound I; 2, hydrolyzing the compound I under acidic condition, and carrying out posttreatment to obtain a compound II; 3, performing catalytic hydrogenation reaction on the compound II to obtain L-tertiary leucine, wherein the catalyst A is magnesium diiodide, magnesium dibromide, magnesium dichloride, magnesium perchlorate or magnesium trifluoromethanesulfonate. The stereochemistry of the L-tertiary leucine can be constructed with high selectivity under solvent-free condition by adopting a magnesium catalyzed three-component one-pot asymmetric cyanosilylation reaction through cheap and easily available organic raw materials.

The invention discloses a printing and dyeing industry sewage treating agent and a preparation method thereof. The printing and dyeing industry sewage treating agent is prepared from, by weight, 20-25 parts of diatomite, 0.5-2 parts of alpha-bromocinnamaldehyde, 1-5 parts of Boc-L-tert-leucine, 5-8 parts of calcium carbonate, 10-25 parts of coal gangue, 5-8 parts of tobacco stalk, 1-3 parts of sodium tartrate, 0.5-5 parts of (diphenylhydroxymethyl)pyrrolidine, 8-15 parts of fly ash, 5-10 parts of wormcast and 5-10 parts of ethylenediaminetetraacetic acid disodium magnesium salt. According to the printing and dyeing industry sewage treating agent, a synergistic effect is exerted through raw material compounding, the content of COD, BOD, SS and NH3-N in printing and dyeing sewage can be effectively decreased, and the chromaticity and the dye concentration of the printing and dyeing sewage can be significantly reduced; the preparation method and the sewage treatment method are simple, the raw materials are easy to obtain, the cost is low, and the sewage treating agent is safe and effective; in addition, the printing and dyeing industry sewage treating agent has the advantages of being capable of rapidly achieving the effects and free of secondary pollution.

The invention discloses a synthetic method of an atazanavir intermediate of N-1-[N-(methoxycarbonyl)-L-tert-leucine]-N-2-[4-(2-pyridyl)-benzyl] hydrazine. The synthetic method comprises (1) amidationreaction, (2) condensation and hydrogenation reaction and (3) condensation reaction. L-tert-leucine and methylchloroformate are subjected to amidation reaction to obtain N-methoxycarbonyl-L-tert-leucine, then 4-(2-pyridyl)-benzaldehyde and hydrazine hydrate are subjected to condensation reaction and hydrogenation reaction to obtain 2-[4-(2-pyridyl)-benzyl] hydrazine hydrochloride, and the 2-[4-(2-pyridyl)-benzyl] hydrazine hydrochloride and the N-methoxycarbonyl-L-tert-leucine are subjected to condensation reaction to obtain the atazanavir intermediate of the N-1-[N-(methoxycarbonyl)-L-tert-leucine]-N-2-[4-(2-pyridyl)-benzyl] hydrazine. The synthetic method of the atazanavir intermediate has the advantages of being low in cost, mild in reaction conditions, low in toxicity, convenient to operate, simple in raw material acquisition, high in yield and applicable to industrial production.