Non-animal-origin frozen semen diluent

A diluent, animal-derived technology, applied in the field of frozen semen diluent, can solve the problems of low acrosome integrity rate, large number of pathogenic microorganisms, and short survival time in thin tube frozen semen, so as to achieve a small number of pathogenic microorganisms and high sperm motility. , the effect of long survival time

Inactive Publication Date: 2013-03-27
辽宁省辽宁绒山羊原种场有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0002] At present, when making frozen semen of cattle and sheep, it is necessary to dilute the semen with a diluent, and the currently used frozen semen diluents all contain animal-derived ingredients (including egg yolk, milk, etc.), and the frozen semen made with it is thawed Sperm motility is low, survival time is short, acrosome integrity rate of thin-tube frozen semen is low, and the number of pathogenic microorganisms is large

Method used

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Embodiment Construction

[0007] The animal source-free frozen semen diluent is prepared from the following ingredients in the following mass ratio: 3.412 grams of lactose, 0.5 grams of sucrose, 0.9 grams of glucose, 0.61 grams of trehalose, 0.53 grams of citric acid, and 0.403 grams of tetraethylamine oxalate. gram, 1.425 grams of trishydroxymethyl aminomethane, 0.375 grams of trisodium citrate, 5.2 milliliters of ethylene glycol, 100 milliliters of double distilled water, 0.88 grams of powdered phospholipids;

[0008] The specific preparation method is as follows: put the described lactose, sucrose, glucose, trehalose, citric acid, tetraethylamine oxalate, trishydroxymethylaminomethane, trisodium citrate, ethylene glycol into the Erlenmeyer flask, Add double-distilled water and heat to 95°C to dissolve the above-mentioned medicines completely. When the temperature is lowered to 35°C, add powdered phospholipids and stir for 30 minutes. Filter paper to filter out the precipitate in the solution, ready ...

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Abstract

The invention relates to a non-animal-origin frozen semen diluent, and relates to a frozen semen diluent prepared with non animal-origin component. The diluent is used for preparing cattle and sheep frozen semen. In prior arts, adopted frozen semen contains animal-origin components, and prepared frozen semen has the defects of low motility and short survival time, low intact acrosome rate of thin tube frozen semen, and large amount of pathogenic micro-organisms after thawing. With the diluent provided by the invention, the problems are solved. The diluent is prepared from the components of, by mass, 3.412 g of lactose, 0.5 g of sucrose, 0.9 g of glucose, 0.61 g of trehalose, 0.53 g of citric acid, 0.403 g of acetic acid tetraethylammonium, 1.425 g of tris(hydroxymethyl) aminomethanegrams, 0.375 g of tri-sodium citrate, 5.2 ml of ethylene glycol, 100 ml of double-distilled water, and 0.88 g of phospholipids powder. With the diluent, prepared frozen semen has the advantages of high motility and long survival time, high intact acrosome rate of thin tube frozen semen, and small amount of pathogenic micro-organisms after thawing.

Description

Technical field: [0001] The invention relates to a diluent of frozen semen prepared with no animal source components used in making frozen semen of cattle and sheep. Background technique: [0002] At present, when making frozen semen of cattle and sheep, it is necessary to dilute the semen with a diluent, and the currently used frozen semen diluents all contain animal-derived ingredients (including egg yolk, milk, etc.), and the frozen semen made with it is thawed The sperm motility is low, the survival time is short, the acrosome integrity rate of the thin tube frozen semen is low, and the number of pathogenic microorganisms is large. Under the premise of 70% sperm motility, the motility of frozen semen is 30-50% after thawing, and the average motility is only 45%. The average survival time at 38°C is 4-7 hours. The average integrity rate is below 65%, and the average number of pathogenic microorganisms after 24 hours of cultivation is 600-1200 colonies. Invention conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 张世伟刘兴伟宋先忱韩迪
Owner 辽宁省辽宁绒山羊原种场有限公司
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