Non-animal-origin frozen semen diluent

Abstract

The invention relates to a non-animal-origin frozen semen diluent, and relates to a frozen semen diluent prepared with non animal-origin component. The diluent is used for preparing cattle and sheep frozen semen. In prior arts, adopted frozen semen contains animal-origin components, and prepared frozen semen has the defects of low motility and short survival time, low intact acrosome rate of thin tube frozen semen, and large amount of pathogenic micro-organisms after thawing. With the diluent provided by the invention, the problems are solved. The diluent is prepared from the components of, by mass, 3.412 g of lactose, 0.5 g of sucrose, 0.9 g of glucose, 0.61 g of trehalose, 0.53 g of citric acid, 0.403 g of acetic acid tetraethylammonium, 1.425 g of tris(hydroxymethyl) aminomethanegrams, 0.375 g of tri-sodium citrate, 5.2 ml of ethylene glycol, 100 ml of double-distilled water, and 0.88 g of phospholipids powder. With the diluent, prepared frozen semen has the advantages of high motility and long survival time, high intact acrosome rate of thin tube frozen semen, and small amount of pathogenic micro-organisms after thawing.

Description

Technical field: [0001] The invention relates to a diluent of frozen semen prepared with no animal source components used in making frozen semen of cattle and sheep. Background technique: [0002] At present, when making frozen semen of cattle and sheep, it is necessary to dilute the semen with a diluent, and the currently used frozen semen diluents all contain animal-derived ingredients (including egg yolk, milk, etc.), and the frozen semen made with it is thawed The sperm motility is low, the survival time is short, the acrosome integrity rate of the thin tube frozen semen is low, and the number of pathogenic microorganisms is large. Under the premise of 70% sperm motility, the motility of frozen semen is 30-50% after thawing, and the average motility is only 45%. The average survival time at 38°C is 4-7 hours. The average integrity rate is below 65%, and the average number of pathogenic microorganisms after 24 hours of cultivation is 600-1200 colonies. Invention conten...

AI Technical Summary

Problems solved by technology

[0002] At present, when making frozen semen of cattle and sheep, it is necessary to dilute the semen with a diluent, and the currently used frozen semen diluents all contain animal-derived ingredients (including egg yolk, milk, etc.), and the frozen semen made with it is thawed Sperm motility is low, survival time is short, acrosome integrity rate of thin-tube frozen semen is low, and the number of pathogenic microorganisms is large