Method for producing flavoring material by double-bacteria fermentation bone paste
A technology of bacterial fermentation and bone paste, applied in the fields of biotechnology and food processing, can solve the problems of high content of bitter peptides in the product, uncontrollable enzymatic hydrolysis process and high enzymatic hydrolysis cost, and achieve high added value, low cost and simple operation. Effect
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Embodiment 1
[0023] Aspergillus oryzae was purchased from ATCC with accession number 42149. Take 4000g of processed fresh pig bone mud, inoculate Aspergillus oryzae separately according to the inoculation amount of 3%, the bone mud concentration is 50%, the initial pH is 7, the fermentation temperature is 30°C, and the fermentation time is 48h. The fermented liquid was centrifugally filtered, tasted delicious, and the degree of hydrolysis was determined to reach 38.2%, and the content of umami amino acids (high performance liquid chromatography) and peptides (Sephadex gel chromatography) was determined to be 6.2 times higher than before fermentation. The content of bitter amino acids (HPLC) decreased by 2.3 times.
Embodiment 2
[0025] Bacillus subtilis was purchased from ATCC with accession number 6633. Take 4000g of processed fresh pork bone paste, inoculate Bacillus subtilis separately according to the inoculation amount of 3%, the concentration of bone paste is 50%, the initial pH is 7, the fermentation temperature is 30°C, and the fermentation time is 48h. The fermented liquid was centrifugally filtered, tasted delicious, and the degree of hydrolysis was determined to be 44.8%. The content of umami amino acids and umami peptides increased by 8.2 times compared with that before fermentation, and the content of bitter amino acids decreased by 2.5 times.
Embodiment 3
[0027] Take 4000g of processed fresh pork bone paste, according to the inoculation ratio (Aspergillus oryzae ATCC42149: Bacillus subtilis ATCC 6633) 2:1, the inoculation amount is 3%, the material-water ratio is 2:3, the initial pH is 7, and the fermentation temperature is 30°C. Fermentation time 48h. The fermented liquid was centrifugally filtered, tasted delicious, and the degree of hydrolysis was determined to reach 52.3%. The content of umami amino acids and umami peptides was measured to increase by 10.4 times compared with that before fermentation, and the content of bitter amino acids decreased by 4.2 times.
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