Preparation method of silicon-nanowire-based medicinal carrier

A technology of silicon nanowires and drugs, applied in the field of nanobiomedicine, can solve the problems of cancer treatment limitations, achieve the effect of inhibiting tumor growth, improving loading efficiency, and killing cancer cells

Active Publication Date: 2013-03-27
SUZHOU UNIV
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Problems solved by technology

Traditional anticancer drugs, such as doxorubicin hydrochloride (DOX) and paclitaxel (PTX), will be distributed in normal tissues and can be quickly cleared by the kidneys, and only a very small amount of anticancer drugs can accumulate in the cancer site to play a role ( Clin.Cancer.Res.1999,5,1703-1707;Pharm.Res.2003,20,1337-1350;Clin.Cancer.Res.2005,11,8782-8788;Nat.Rev.Cancer. 592.), thus being greatly limited in cancer treatment

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  • Preparation method of silicon-nanowire-based medicinal carrier
  • Preparation method of silicon-nanowire-based medicinal carrier

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preparation example Construction

[0021] The preparation method of the drug carrier based on silicon nanowires of the present invention specifically comprises the following steps:

[0022] Use a buffer solution to make silicon nanowires into a system of 20-200 micrograms per milliliter, add antineoplastic drugs to mix into a solution, shake at 20-37 degrees Celsius for 3-30 hours, centrifuge and wash 3-10 times with the same buffer solution, remove The unbound anti-tumor drug in the upper layer is precipitated in the lower layer, which is the silicon nanowire-anti-tumor drug complex.

[0023] Preferably, the silicon nanowires have a diameter of 50-150 nanometers and a length of 250-750 nanometers.

[0024] Preferably, the pH of the buffer solution is 4-10.

[0025] Preferably, the buffer solution is PB buffer, PBS buffer or carbonate buffer.

[0026] Preferably, the antitumor drug is doxorubicin hydrochloride or paclitaxel.

Embodiment 1

[0030] (1) Preparation of SiNW-DOX composite

[0031] Add 20 micrograms of SiNWs solids to 100 microliters of PB buffer solution with pH=9, and put them together with 1 milliliter of 640 micrograms per milliliter of DOX solution into a 1.5 milliliter centrifuge tube, and react in a constant temperature shaker at 25 degrees Celsius for 12 After one hour, centrifuge in a centrifuge and wash with PB buffer to obtain a SiNW-DOX complex solution for use.

[0032] (2) DOX loading efficiency

[0033] The supernatant solution containing free DOX after centrifugation was collected, and the absorbance at 490 nanometers was measured by colorimetry using an ultraviolet-visible-near-infrared spectrometer, and the calculated loading efficiency was about 20800 micrograms per gram.

[0034] (3) Cytotoxicity study of SiNW-DOX complex

[0035] The high-sugar DMEM medium containing 20 micrograms per milliliter of SiNW-DOX complex was added to a 96-well cell culture plate containing HeLa cells ...

Embodiment 2

[0037] (1) Preparation of SiNW-DOX composite

[0038] Add 20 micrograms of SiNWs solid to 100 microliters of PBS buffer solution with pH=9, and then put it into a 1.5 milliliter centrifuge tube together with 1 milliliter of 700 micrograms per milliliter of DOX solution, and react 24 times in a constant temperature shaker at 25 degrees Celsius. After one hour, centrifuge in a centrifuge and wash with PBS buffer to obtain a SiNW-DOX complex solution for use.

[0039] (2) DOX loading efficiency

[0040] The supernatant solution containing free DOX after centrifugation was collected, and the absorbance at 490 nanometers was measured by colorimetry using an ultraviolet-visible-near-infrared spectrometer, and the calculated loading efficiency was 19,000 micrograms per gram.

[0041] (3) Cytotoxicity study of SiNW-DOX complex

[0042] The improved -1640 medium containing 5 micrograms per milliliter of SiNW-DOX complex was added to a 96-well cell culture plate containing KB cells an...

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Abstract

The invention discloses a preparation method of a silicon-nanowire-based medicinal carrier. The method comprises the following steps of: preparing a system of between 20 and 200mg / ml from the silicon nanowire with buffer; adding an antitumor medicament to obtain mixed liquid; oscillating for 3 to 30 hours at a temperature of between 20 and 37 DEG C; centrifuging, and washing with the same buffer for 3 to 10 times; and removing the antitumor medicament which is not combined on the upper layer to obtain the lower precipitate, which is silicon nanowire-antitumor medicinal composition. According to the method, the load efficiency of a traditional antitumor medicament in a carrier can be greatly improved along with safe operation, quickness and convenience; and the obtained silicon nanowire-antitumor medicinal composition can play an excellent role in killing cancer cells on cell and living body levels, and plays an effect of medicament sustained release, so that tumor growth can be inhibited in a long time. The silicon-nanowire-based medicinal carrier can be used as a nano-medicament due to the great loading efficiency of the silicon nanowire, and can be widely applied to the field of cancer treatment.

Description

technical field [0001] The invention belongs to the technical field of nanometer biomedicine, and relates to a preparation method of a nanomaterial drug carrier, in particular to a preparation method of a silicon nanowire-based drug carrier. Background technique [0002] With the rapid development of nanobiotechnology, nanomaterials as drug carriers for cancer therapy have received extensive attention in recent years. Traditional anticancer drugs, such as doxorubicin hydrochloride (DOX) and paclitaxel (PTX), will be distributed in normal tissues and can be quickly cleared by the kidneys, and only a very small amount of anticancer drugs can accumulate in the cancer site to play a role ( Clin.Cancer.Res.1999,5,1703-1707;Pharm.Res.2003,20,1337-1350;Clin.Cancer.Res.2005,11,8782-8788;Nat.Rev.Cancer. 592.), thus being greatly limited in cancer treatment. [0003] In order to increase the drug concentration of antitumor drugs at the site of action, one of the effective strategies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/04A61K45/00A61K31/704A61K31/337A61P35/00
Inventor 何耀彭飞苏媛媛李述汤
Owner SUZHOU UNIV
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