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Pseudolarix amabilis cell suspension culture method

A technique of suspension culture and money pine, which is applied in the field of suspension culture of money pine cells, can solve the problems of difficulty and long cycle, and achieve the effect of solving the shortage of plant resources and medicinal material resources

Inactive Publication Date: 2013-03-27
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for suspension culture of pine cells in response to the current shortage of pine resources and the long and difficult problems of the existing traditional cultivation methods, which can quickly produce active ingredients in a short time Cell culture of pine tree with high content to alleviate the current shortage of pine plant resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Induction culture of callus: Select the buds on the pine plants that grow healthy after falling leaves and have no diseases and insect pests, peel off the bud scales on the buds and put the buds on the ultra-clean workbench, first use a concentration of 70% sterilized with alcohol for 40 s, then sterilized with 0.1% mercuric chloride for 4 min, and finally rinsed with sterile water for 4 times, and then inoculated the sterilized buds into solid medium MS+6-BA1.0 mg·L -1 +NAA2.0 mg·L -1 + 30 g·L sucrose -1 + agar 6.0 g·L -1 cultured in 22 ° C, pH value 6, 10 hours of light per day, and light intensity of 1500 lx, the induction rate of callus after 25 days of culture was 98.2%;

[0020] (2) Pre-culture of cells: transfer the emerald green callus obtained in step (1) into liquid medium MS+6-BA1.0 mg·L -1 +2, 4-D 3.0 mg L -1 +KT 0.5mg·L -1 + sucrose 25 g·L -1 7 days of pre-cultivation;

[0021] (3) Suspension culture of cells: select single cells or small a...

Embodiment 2

[0026] Change the pre-medium in step (2) of Example 1 to MS+6-BA 1.2 mg·L -1 +2, 4-D 2.0 mg·L -1 +KT 1 mg·L -1 + 30 g·L sucrose -1 , other steps are the same as in Example 1, and the biomass multiplier of the 3 generations of subcultured pine cells is 1.88 times, and the growth rate of the cells is 2.14 g (d L) -1 .

Embodiment 3

[0028] Change the pre-medium in step (2) of Example 1 to MS+6-BA 1.0 mg·L -1 +2, 4-D 3.0 mg L -1 + sucrose 25 g·L -1 , other steps are the same as in Example 1, and the biomass multiplier of the 3 generations of subcultured pine cells is 2.12 times, and the growth rate of the cells is 2.61 g (d L) -1 .

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PUM

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Abstract

The invention discloses a pseudolarix amabilis cell suspension culture method. The method comprises the following steps of: callus induction culture, cell preculture, cell suspension culture and cell subculture. According to the method, pseudolarix amabilis callus is adopted to carry out the cell suspension culture, so that pseudolarix amabilis cell cultures with high effective ingredient content can be quickly produced in large scale.

Description

technical field [0001] The invention relates to a method for tissue culture of pine pine, in particular to a method for suspension culture of pine cells. Background technique [0002] The golden pine is an ancient and relict species of Pinaceae endemic to my country. According to the new threatened species classification system standards of the World Conservation Union (IUCN) and the standards of the Chinese Plant Red Book, the golden pine is designated as a endangered species and listed as a national second-class protected plant. [0003] The golden pine is not only an important garden plant, but also an important medicinal plant with broad application prospects. Studies have found that the most important medicinal ingredient of pine pine is the diterpenoid compound hibiscus acid extracted from its bark and root bark. It is the anti-tumor effect that makes the medicinal value of jinsong more prominent. Modern pharmacological research has found that hibiscus acid and its ...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N5/02
Inventor 王跃华杨小萍袁畅王强黄兴王丹宋超覃泽娇王朝君吴佳靓熊云翔江明殊
Owner CHENGDU UNIV