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Preparation method of high-purity triglyceride type PUFA (Polyunsaturated Fatty Acid)

A triglyceride, high-purity technology, applied in the field of preparation of high-purity triglyceride-type PUFA, can solve the problem of low triglyceride purity, achieve good economy and environmental protection, and avoid oxidation

Active Publication Date: 2014-06-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortcoming that the purity of triglycerides in the PUFA glyceride products obtained by existing synthetic methods is not high, the purpose of the present invention is to provide a method for preparing high-purity triglyceride-type PUFAs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Add glycerin 100g, PUFA concentrate 1000g (from tuna oil, PUFA composition is ALA 1.6%, AA 9.8%, EPA 7.8%, DPA 16.4%, DHA 50.8%), 10g lipase Novozym435, 20g molecular sieve (remove The moisture generated by the esterification reaction), the esterification reaction was carried out in a constant temperature magnetic stirrer at 50° C. at a stirring speed of 200 rpm. After the reaction is over, the reaction mixture is centrifuged or filtered to separate Novozym435, centrifuged to remove unreacted glycerol, and the esterification reaction product is recovered. The triglyceride content in the esterified product was analyzed by liquid chromatography to be 69.7%, the diglyceride content was 11.2%, the monoglyceride content was 4.7%, and the remaining fatty acid was 14.4%. After testing, the peroxide value of the oil phase is 2.1meq / Kg. Add 100g water and 10g partial glyceride lipase Lipase SMG1 to the esterification product (Wang W-f, Li T, Qin X-l, Ning Z-x, Yang B, Wang Y-h....

Embodiment 2

[0023] Add glycerin 100g, PUFA concentrate 1000g (from tuna oil, PUFA composition is ALA 1.6%, AA 9.8%, EPA 7.8%, DPA 16.4%, DHA 50.8%), 10g lipase Novozym435, 20g molecular sieve to remove reaction vessel The generated moisture was esterified in a constant temperature magnetic stirrer at 50° C. at a stirring speed of 200 rpm. After the reaction is over, the reaction mixture is centrifuged or filtered to separate Novozym435, centrifuged to remove unreacted glycerol, and the esterification reaction product is recovered. The triglyceride content in the esterified product was analyzed by liquid chromatography to be 69.7%, the diglyceride content was 11.2%, the monoglyceride content was 4.7%, and the remaining fatty acid was 14.4%. After testing, the peroxide value of the oil phase is 2.1meq / Kg. 100 g of water and 10 g of partial glyceride lipase Lipase SMG1 were added to the esterification product, and the hydrolysis reaction was carried out in a constant temperature magnetic st...

Embodiment 3

[0025]Add 100g of glycerin, 900g of CLA ethyl ester concentrate (CLA content is 80.3%), 10g of lipase Novozym435 into the reaction vessel, and carry out esterification at a constant temperature magnetic stirrer at 50°C at a stirring speed of 200rpm under a vacuum of 100Pa reaction. After the reaction is over, the reaction mixture is centrifuged or filtered to separate Novozym435, centrifuged to remove unreacted glycerol, and the esterification reaction product is recovered. The content of triglyceride in the esterified product was analyzed by liquid chromatography to be 70.5%; the content of diglyceride was 11.5%; the content of monoglyceride was 4.4%; and the remaining fatty acid was 13.6%. After testing, the peroxide value of the oil phase is 2.0meq / Kg. 100 g of water and 10 g of partial glyceride lipase Lipase SMG1 were added to the esterification product, and the hydrolysis reaction was carried out in a constant temperature magnetic stirrer at 2° C. at a stirring speed of...

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PUM

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Abstract

The invention discloses a preparation method of high-purity triglyceride type PUFA (Polyunsaturated Fatty Acid). The preparation method comprises the following steps: (1), using lipase Novozym435 as catalyst, enabling acyl donor of the PUFA to react with glycerol; removing catalyst and residual glycerol to obtain a mixture of trimester type PUFA and meta-glyceride PUFA; (2), carrying out hydrolysis or alcoholysis reaction to the meta-glyceride PUFA and acyl donor in catalytic mixture at a temperature not higher than 5 DEG C through the meta-glyceride lipase SMG1; (3), separating the reaction product to recycle oil phase, and purifying the oil phase to obtain the high-purity triglyceride type PUFA. The preparation method uses meta-glyceride lipase to selectively remove non-triglyceride components in the PUFA triglyceride at a low temperature to obtain a PUFA glyceride product with a low peroxide value and triglyceride content of 95-100%, and has better economical efficiency and environmental protection capability.

Description

technical field [0001] The invention relates to a preparation method of high-purity triglyceride type PUFA. Background technique [0002] Polyunsaturated fatty acids (PUFA) generally refer to straight-chain fatty acids containing two or more double bonds and a carbon chain length of 18 to 22 carbon atoms. Important PUFAs include eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), eicosapentaenoic acid (DPA), arachidonic acid (AA), stearidonic acid ( SDA), α-linolenic acid (ALA), γ-linolenic acid (GLA). PUFA has entered the field of biopharmaceuticals and nutraceuticals due to its unique biological activity. Most of these PUFAs are derived from marine fish oils and plants. In the past two decades, people have increasingly realized that the deficiency of PUFAs is associated with certain diseases. In addition to their potential pharmaceutical applications, the presence of PUFAs in food sources and the public The emphasis on healthy food has caused these compounds to att...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
Inventor 王永华王卫飞杨博蓝东明李志刚凌飞
Owner SOUTH CHINA UNIV OF TECH