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Method for preparing deep-sea bacterial polysaccharides in low cost

A deep-sea bacteria, low-cost technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high fermentation cost, affecting the development and application of polysaccharides, etc., to achieve lower fermentation costs, good environmental benefits and social benefits. Benefit, the effect of reducing fermentation cost

Inactive Publication Date: 2013-03-27
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of using lactose and peptone to ferment the polysaccharide is relatively high, which seriously affects the development and application of the polysaccharide

Method used

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  • Method for preparing deep-sea bacterial polysaccharides in low cost
  • Method for preparing deep-sea bacterial polysaccharides in low cost
  • Method for preparing deep-sea bacterial polysaccharides in low cost

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method of deep-sea bacterial polysaccharide, the steps are as follows:

[0039] (1) The deep-sea bacteria (Zunongwangia profunda) SM-A87 strain was inoculated in the liquid seed medium, and shaken at 20°C for 3 days to obtain the seed culture solution;

[0040] The components of the liquid seed culture medium are as follows, all in parts by weight:

[0041] 0.8 parts of peptone, 0.75 parts of yeast powder, 100 parts of artificial seawater, pH 8.0;

[0042] (2) Inoculate the seed culture solution prepared in step (1) on the fermentation medium at a volume percentage of 5%, and culture it for 20 hours at 20°C and the dissolved oxygen saturation is higher than 30%, and then adjust the fermentation temperature to Continue culturing at 12°C for 8 days, and add whey concentrate during the period of continuing culturing to obtain a fermented liquid;

[0043] The components of the fermentation medium are as follows, whey is parts by volume, and the unit is in mi...

Embodiment 2

[0056] A preparation method of deep-sea bacterial polysaccharide, the steps are as follows:

[0057] (1) The deep-sea bacteria (Zunongwangia profunda) SM-A87 strain was inoculated in the liquid seed medium, and shaken at 15°C for 3 days to obtain the seed culture solution;

[0058] The components of the liquid seed culture medium are as follows, all in parts by weight:

[0059] 1.0 parts of peptone, 0.5 parts of yeast powder, 100 parts of artificial seawater, pH 7.5;

[0060] (2) Inoculate the seed culture solution prepared in step (1) on the fermentation medium at a volume percentage of 3%, and culture it for 30 hours at 15°C and the dissolved oxygen saturation is higher than 30%, and then adjust the fermentation temperature to Continue culturing at 8°C for 10 days, and add whey concentrate during the period of continuing culturing to obtain a fermented liquid;

[0061] The components of the fermentation medium are as follows, whey is parts by volume, and the unit is in mil...

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Abstract

The invention relates to a method for preparing deep-sea bacterial polysaccharides in low cost, comprising the following steps: (1) inoculating a deep-sea bacteria SM-A87 strain into a liquid seed culture medium, and culturing by shaking to obtain a seed nutrient solution; (2) inoculating the seed nutrient solution into a fermentation culture medium, culturing, then adjusting the fermentation temperature to be continuously cultured, and additionally adding a whey concentrated solution to prepare a fermentation solution; (3) adding anhydrous ethanol into the fermentation solution for ethanol precipitation, separating to obtain a precipitate, washing by anhydrous ethanol, and drying to prepare a crude polysaccharide; and (4) dissolving the crude polysaccharide into water, adding alkaline protease for enzymolysis, adding ethanol for ethanol precipitation, separating to obtain the precipitate, washing by anhydrous ethanol, and drying to prepare deep-sea bacteria exopolysaccharide. According to the method, lactose is replaced by cheese processing byproducts of whey liquid, and peptone is replaced by bean pulps to ferment the bacterial strain SM-A87 exopolysaccharide, and the fermentation cost is reduced by more than 70%.

Description

technical field [0001] The invention relates to a low-cost preparation method of deep-sea bacterial polysaccharides, in particular to a method for preparing deep-sea bacterial polysaccharides by using cheese processing by-product whey liquid and soybean meal, and belongs to the technical field of biotechnology. Background technique [0002] Bacterial extracellular polysaccharides include capsular polysaccharides and mucus polysaccharides, which have attracted much attention due to their excellent properties such as safety, non-toxicity, unique physical and chemical properties, wide application, easy separation from bacteria, and industrial production through submerged fermentation. Compared with plant polysaccharides, they have a wide variety, short production cycle, and are not restricted by seasons, regions, and pest conditions. The advantages of these production links of bacterial polysaccharides and the superior physical and chemical properties of the product have brough...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01
Inventor 张玉忠刘升波陈秀兰秦启龙苏海楠宋晓妍周百成
Owner SHANDONG UNIV