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Multifunctional plant excised root culture bioreactor system

The technology of a bioreactor and a reactor system is applied in the field of a multifunctional plant in vitro root culture bioreactor system, which can solve the problems that the advantages cannot be reflected at the same time, and achieve the effects of good gas-liquid mixing effect and short mass transfer ability.

Inactive Publication Date: 2015-01-14
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the currently commonly used bubble column and spray reactors are independent types, which cannot embody the advantages of both at the same time.

Method used

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  • Multifunctional plant excised root culture bioreactor system

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Embodiment

[0020] The multifunctional plant isolated root culture bioreactor system of the present embodiment is as figure 1 Shown, comprise spray device and reactor, described spray device comprises spray tank 2, siphon pipe 8, air spray head 9, liquid inlet pipe 18, air filtering device 16, spray head 1, spray valve 3 and liquid pump 17, Described reactor comprises reaction tank 6 (total volume is 2 liters, height-to-diameter ratio (H / D) is 4: 1), stirring paddle 7, net 13, air-spray pad 10 (air-spray pad selects aperture to be 100~180 μm Glass filter core, preferably aperture is the glass filter core of 180 μ m) and liquid outlet pipe 19; Described air nozzle 9 is arranged on the bottom of described spray liquid storage tank 2, and described liquid inlet pipe 18 and air filtering device 16 are respectively arranged Above the spray liquid storage tank 2, the liquid inlet pipe 18 is provided with a liquid inlet valve 4, and the bottom of the spray liquid storage tank 2 is connected to t...

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Abstract

The invention discloses a multifunctional plant excised root culture bioreactor system which comprises a spray device and a reactor, wherein the spray device comprises a spray liquid storage tank, a siphon tube, a gas sprayer, a liquid inlet tube, an air filter, a spray head, a spray valve and a liquid pump; the reactor comprises a reaction tank, a stirring paddle, a net, a gas spray cushion and a liquid outlet tube; the liquid inlet tube and the air filter are respectively arranged above the spray liquid storage tank; the lower part of the spray liquid storage tank is sequentially connected with the liquid pump and the lower part of the reaction tank through a liquid delivery duct; both ends of the siphon tube are respectively arranged in the spray liquid storage tank and the reaction tank; the end of the siphon tube extending into the reaction tank is provided with the spray head; the lower part of the reaction tank is provided with a liquid outlet connected with the liquid outlet tube; and the reaction tank is sequentially provided with the gas spray cushion, stirring paddle and net from bottom to top. The reactor system disclosed by the invention can implement two culture modes (bubble tower and spray) in one reactor.

Description

technical field [0001] The invention relates to a system in the field of biotechnology, in particular to a multifunctional plant isolated root culture bioreactor system. Background technique [0002] The large-scale cultivation of isolated roots of medicinal plants (adventitious roots, hairy roots, etc.) in bioreactors can not only produce many valuable secondary metabolites, but also alleviate the over-harvesting of wild resources. At present, the reactors used for in vitro root cultivation of medicinal plants mainly include bubbling type, air lift type, fluidized bed type and spray type. Bubble towers and spray reactors have the characteristics of low shear force and strong mass transfer capacity, and are the main types of reactors used in plant isolated root culture. [0003] The law of plant isolated root growth in the reactor conforms to the "S" curve. After inoculation, the isolated roots of plants need about 10 days to adapt to the surrounding environment, so the bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34C12M1/02
Inventor 王贵荣
Owner SHANGHAI JIAOTONG UNIV
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