A rapid preparation method for ex vivo dyed epidermal sheets of macroalgae

A lamella and epidermis technology, which is applied in the field of rapid production of large seaweed in vitro dyed epidermis, can solve the problem that the production of dissociated large seaweed epidermis has not yet been seen, the whole picture of the observation object cannot be displayed, and clear photos cannot be obtained. problem, to achieve the effect of good dyeing effect, not easy to fade, continuous morphological structure difference

Inactive Publication Date: 2015-11-18
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are obvious deficiencies in these methods: 1. Sections can only observe part of the tissue and structure, and cannot show the whole picture of the observed object; 2. Direct observation through the surface, because there are other tissue cells inside, the observation results are often disturbed; 3. Whether it is section or surface observation, the results of ultramicro observation are usually not good, and image information such as clear photos of the desired tissue structure cannot be obtained
So far, there has not been any report about using the characteristics of the reagent to dissociate the epidermis of macroalgae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of mature epidermal sheet of macroalgae

[0026] (1) Select the adult part of the sample, and clean the surface of the algae with a brush and an ultrasonic cleaner for 2-3 times. The ultrasonic cleaner usually processes the sample for 8-10 minutes each time to remove impurities and algae on the surface of the algae, and cut into long For the sample segment of about 1-1.5cm, select the sample with uniform growth, moderate thickness, and few branches for subsequent operations.

[0027] (2) Transfer the cleaned and cut samples into the groove of the glass slide or into a small petri dish or beaker, generally add 1 drop of mixed reagent for 4 samples, and process the samples for 10-12 minutes, every 3- Turn the sample once every 4 minutes to fully dissociate and stain the epidermis and endothelial cells of the sample.

[0028] (3) Transfer the stained and dissociated sample into a small beaker, wash the sample 3-4 times with distilled water, remove...

Embodiment 2

[0031] Embodiment 2: Preparation of the tender epidermal sheet of macroalgae

[0032] (1) Select the adult part of the sample, clean the surface of the algae body once with an ultrasonic cleaner, and the treatment time is generally 3-4 minutes to remove impurities on the surface of the algae body, cut into a sample segment about 1-1.5cm in length, and select the growth in it Samples that are uniform, moderate in thickness, and less branched are used for subsequent operations.

[0033] (2) Transfer the cleaned and cut samples into the slide groove or into a small petri dish or beaker, generally add 1 drop of mixed reagent for 8 samples, and process the samples for 10 minutes, every 3-4 minutes Flip the sample once to fully dissociate and stain the epidermal cell layer and endothelial layer cells of the sample.

[0034] (3) Transfer the stained and dissociated sample into a small beaker, wash the sample 2-3 times with distilled water, remove the mixed reagents on the surface ...

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Abstract

The invention discloses a rapid tableting method of a large-sized alga in-vitro dyeing epidermal lamella. The method comprises the following steps of: preparing a mixing reagent; completing dissociation at one step, and dyeing a large-sized alga epidermal lamella; and manually operating to rapidly prepare an epidermal cell lamella of which the area can be up to 1cm<2>. The method is easy and convenient to operate, has a high preparation speed, a good color development effect and high stability, and is suitable for observation through microscopes and electron microscopes and observation and research of epidermal cell ultrastructures.

Description

technical field [0001] The invention relates to a film-making method in the study of the microstructure and ultra-microstructure of organisms, in particular to a rapid film-making method for in vitro dyed epidermis of large seaweed. Background technique [0002] Microscopic and ultrastructural research is a necessary means to reveal life phenomena and processes. It has been more than 300 years since the Dutch scientist Hooke invented the microscope, and many progresses have been made. However, the research in this field is often limited by the preparation technology in the sample processing process. good or bad. [0003] Large seaweed is a large low-level plant in the ocean. Its length ranges from a few millimeters to tens of meters. Most of the common ones are centimeter-level, and the thickness is generally only a few millimeters long. Common large seaweeds are relatively small in size. Then, the study of its microstructure and ultrastructure has become an indispensable l...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
Inventor 丁兰平黄冰心
Owner SHANTOU UNIV
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