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Nacre glycosaminoglycan soft capsules for enhancing immunity and preparation method thereof

The invention relates to a technology for enhancing immunity by glycosaminoglycan and a technology for enhancing immunity, which is applied in the fields of glycosaminoglycan soft capsules and preparation thereof, and the field of the preparation of glycosaminoglycan soft capsules in N. , to achieve the effect of high product quality, convenient operation and high safety

Inactive Publication Date: 2013-04-17
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a pinnacle glycosaminoglycan soft capsule for enhancing immunity, to solve the environmental problem of resource waste of pinnacles martensii, and to make comprehensive utilization of it to make products beneficial to human beings

Method used

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  • Nacre glycosaminoglycan soft capsules for enhancing immunity and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0015] Add 250 g of water to homogenate 500 g (wet weight) of Pinctada martensii whole organs, add trypsin 1% (w / w) and subtilisin neutral protease 0.5% (w / w) according to the mass ratio, at pH 7.0, 50°C constant temperature water bath for 3 hours, high temperature 100°C to inactivate enzyme and cool down, centrifuge at 4000rpm for 5min, take the supernatant and add adsorbent (activated carbon: diatomaceous earth = 3:2) at a mass ratio of 2%, keep the temperature at 65°C for 50min, put on gauze After filtration, centrifuge at 4000rpm for 10min to take the supernatant, add 95% food-grade alcohol for alcohol precipitation, the volume ratio of supernatant to alcohol is 1: 0.5, precipitate at 4°C for 24h, take the precipitate and wash it three times alternately with absolute ethanol and acetone , collect the precipitate and dissolve it in water, adjust the isoelectric point to pH 2.0, centrifuge to remove protein, and then obtain glycosaminoglycan (ie soft capsule raw material) by ...

Embodiment 2

[0017] Homogenize 500g (wet weight) of Pinctada martensii whole organs with 500g water, add trypsin 1%-2% (w / w) and Bacillus subtilisin dispase 1% (w / w) according to the mass ratio, and pH 7.0, 50°C constant temperature water bath for 5 hours, high temperature 100°C to inactivate enzymes and cool down, centrifuge at 4000rpm for 5min, take supernatant and add adsorbent (activated carbon: diatomaceous earth = 3:2) at a mass ratio of 2%, 65°C constant temperature After 50min, filter with gauze, centrifuge at 4000rpm for 10min to take the supernatant, add 95% food-grade alcohol for alcohol precipitation, the volume ratio of supernatant to alcohol is 1: 1.5, precipitate at 4°C for 24h, take the precipitation with absolute ethanol and acetone alternately Wash 3 times, collect the precipitate and dissolve it with water, adjust the isoelectric point to pH 2.0, centrifuge to remove protein, and then obtain the refined product of glycosaminoglycan glycosaminoglycan (ie soft capsule raw m...

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Abstract

The invention relates to nacre glycosaminoglycan soft capsules for enhancing immunity and a preparation method thereof, belonging to the technical field of health food production. The method comprises the steps of adding water into all internal organs of pinctada martensii and homogenizing; adding pancreatin and bacillus subtilis neutral protease; performing constant-temperature water bath at 50 DEG C with pH of 7.0; performing high-temperature enzyme deactivation and cooling; centrifuging, taking supernate and adding an adsorbent; at constant temperature of 65 DEG C, filtering with gauze, centrifuging and taking supernate; adding 95% food-grade alcohol for alcohol precipitation; taking the precipitate and washing with absolute ethyl alcohol and acetone alternately; collecting the precipitate and adding water for dissolution; adjusting the isoelectric point and centrifuging to remove protein; performing ultrafiltration to obtain glycosaminoglycan; adding a medical-grade PEG400 diluent, and adding a suspending agent and propylene glycol at the same time; and sufficiently and uniformly mixing to obtain soft capsules. The soft capsules have the advantages of little toxic and side effect, high safety, obvious effect in improving immune function of body and the like; and the method has simple technology, is convenient to operate, and realizes high product quality.

Description

technical field [0001] The invention relates to a glycosaminoglycan soft capsule and a preparation method thereof, in particular to a pearl oyster glycosaminoglycan soft capsule for enhancing immunity and a preparation method thereof, which belongs to the technical field of health food production and is a kind of A method for preparing soft capsule products with enhanced immune function from active substances isolated from Pinctada martensii. Background technique [0002] Pinctada martensii (Dunder) is the main species of pinctada martensii (Dunder) in the South my country Sea, and it is cultivated in large quantities in some areas of Guangdong and Guangxi. After the pearl farmers collect pearls, the resources of the remaining mother-of-pearl viscera are about 3,500-4,000 tons per year. In areas such as Leizhou City and Xuwen County in Guangdong Province, Beihai City and Qinzhou City in Guangxi Zhuang Autonomous Region, and parts of Hainan Province, most of them are only us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/09A23P1/04
Inventor 吴红棉范秀萍钟敏胡雪琼吴文龙
Owner GUANGDONG OCEAN UNIVERSITY