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Echinococcus granulosus recombinant BCG vaccine and preparation method thereof

A technology of Echinococcus granulosus and Echinococcus granulosus, which is applied in the field of vaccine preparation, can solve the problems of large damage, recurrence, and adverse reactions, and achieve the effects of enhancing immunity, less immunization times, and good thermal stability

Inactive Publication Date: 2013-04-17
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the damage caused by surgery to the human body is relatively large and recurrence is possible, and chemotherapy drugs can cause some serious adverse reactions

Method used

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  • Echinococcus granulosus recombinant BCG vaccine and preparation method thereof
  • Echinococcus granulosus recombinant BCG vaccine and preparation method thereof
  • Echinococcus granulosus recombinant BCG vaccine and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0026] 1 material

[0027] The Escherichia coli-mycobacterium shuttle plasmid pMV361 and BCG used in the present invention and E.coli DH5α bacterial strain can be obtained through commercial channels, and pMV361 and BCG and E.coli DH5α bacterial strain are purchased from Biovector Science lab Co., Ltd. (China plasmid vector Strain Cell Line Gene Collection Center).

[0028] 2 methods

[0029] 2.1 Design and synthesis of primers

[0030] The upstream and downstream primers were designed according to the gene sequence of Echinococcus granulosus egG1Y162 (GenBank accession numbers are AB458258 and AB458259) and the plasmid map of PMV361, and EcoRI and HindⅢ restriction endonuclease sites were introduced.

[0031] Upstream primer: 5′-CC GAATTCATGGTACTTCGATTCTGT-3′, the underlined part is the EcoRI site;

[0032] Downstream primer: 5′-CC AAGCTT AGTAAGTAATAGGAGCCCA-3′, the underlined part is HindⅢ site.

[0033] 2.2 PCR amplification of target gene egG1Y162

[0034] Using Ec...

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Abstract

The invention provides the preparation of an echinococcus granulosus recombinant BCG vaccine. The vaccine is good in heat stability and convenient to transport and store; single immunity can induce high potency antibody aiming at target antigen, so that immunity is durable, immune time is few, immune procedure is simplified, and immune effect aiming at echinococcus granulosus is enhanced.

Description

technical field [0001] The invention provides a recombinant BCG vaccine of echinococcus granulosus, which is used for preventing and controlling echinococcosis granulosus in animals, and also provides a preparation method of the vaccine, which belongs to the technical field of vaccine preparation. Background technique [0002] Cystic echinococcosis (CE) is a zoonotic parasitic disease that seriously endangers people's health. Hydatid cystectomy is currently the treatment of choice for patients with echinococcosis. However, the damage to the human body caused by surgery is relatively large and there is a possibility of recurrence, and chemotherapy drugs can produce some serious adverse reactions. Therefore, more effective solutions need to be studied, and vaccine prevention is an effective measure to prevent and control its prevalence. [0003] Vaccines for the prevention of echinococcosis have undergone various explorations such as peptide vaccines, subunit vaccines, and D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P33/10C12N15/12C12N15/74C12N1/21
Inventor 丁剑冰马海梅祖力皮也·吐尔逊马秀敏曹春宝德里夏提·依米提朱明温浩
Owner XINJIANG MEDICAL UNIV
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