Double-specificity monoclonal antibody resistant to triazophos and chlorpyrifos and preparation method and application thereof

A monoclonal antibody and anti-triazophos technology, applied in chemical instruments and methods, specific peptides, instruments, etc., can solve problems such as toxicity, single analysis technology and means, and achieve stable properties and high detection sensitivity

Inactive Publication Date: 2013-04-17
ZHEJIANG GONGSHANG UNIVERSITY
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Triazophos original drug is moderately toxic and toxic to fish and bees
[0010] Although there are currently reports on the immunoassay techniques for triazophos and chlorpyrifos, they are all limited to a single analysis technique and means. In the context of the need for multi-residue analysis, it is im...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention will be further described below in conjunction with the examples.

[0023] 1. Preparation of Immunogen

[0024] 1.1 Preparation of triazophos hapten, artificial antigen and coated antigen

[0025] 1.1.1 O - Synthesis of ethyl thiophosphoryl dichloride (TZLM-1)

[0026] Weigh the phosphorus trichloride (PSCl3 ) 68 g (about 0.4 mol) was placed in a three-necked flask with a low temperature thermometer, cooled to -10~-5 ℃ with an ice-salt water bath, and 55 g (about 1.2 mol) of absolute ethanol was added dropwise under vigorous stirring, Strictly control the rate of addition so that the temperature of the reaction solution is always not greater than 0 °C. After the dropwise addition, the reaction was continued at 10 °C for 2 h. After the reaction, the reaction solution (100 ml×2) was washed with (0±5)°C distilled water, the oil layer was separated and washed with anhydrous Na 2 SO 4 Dry it, then distill it under reduced pressure with a water pum...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Ionic strengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a double-specificity monoclonal antibody resistant to triazophos and chlorpyrifos and a preparation method and application thereof, and belongs to the technical field of biology. The double-specificity monoclonal antibody has specificity for triazophos and chlorpyrifos simultaneously. The preparation method for the monoclonal antibody comprises the steps as follows: synthesizing half antigens and artificial antigens of triazophos and chlorpyrifos; utilizing the artificial antigens of triazophos to prepare hybridoma cell strains secreting a component resistant to triazophos; utilizing the artificial antigens of chlorpyrifos to prepare hybridoma cell strains secreting a component resistant to chlorpyrifos; and after the triazophos-resistant hybridoma cell strains and hybridoma-resistant hybridoma cell strains are subjected to cell fusion, screening to obtain tetrasomic hybridoma cells which secrete the double-specificity monoclonal antibody resistant to triazophos and chlorpyrifos. The double-specificity monoclonal antibody prepared by the invention has the advantages that the characters are stable, the detection sensitivity is high, the double-specificity monoclonal antibody can be produced massively, and meanwhile the antibody is used for establishing an ELISA (enzyme-linked immuno sorbent assay) analysis method, so that the technical basis for the rapid detection of triazophos and chlorpyrifos is provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bispecific monoclonal antibody against triazophos and chlorpyrifos, a preparation method and application thereof. Background technique [0002] Immunoassay (IA) is a technology that uses antibodies as biochemical detectors to perform qualitative and quantitative analysis of compounds, enzymes, or proteins. It is based on the specific and reversible binding reaction between antigens and antibodies. It has the characteristics of strong specificity, high sensitivity, convenience and speed, high throughput, low detection cost, safety and reliability. This method generally does not require expensive instruments, and the technical requirements of the users are not high. It is easy to popularize and promote, and is especially suitable for on-site screening and rapid analysis of a large number of samples. A series of detection products developed based on this technology, such ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K16/44G01N33/577
Inventor 金仁耀朱国念桂文君
Owner ZHEJIANG GONGSHANG UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap