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Preparation method of autologous platelet-factor-rich plasma (PFRP) preparation

A platelet-rich plasma and platelet-rich technology, applied in the field of biomedicine, can solve the problems of unsustainable filling effect, obvious pain, tissue damage, etc., and achieve the effects of good tensile strength and ductility, simple preparation method and low cost.

Active Publication Date: 2013-05-01
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of animal-derived thrombin and calcium ions may lead to exogenous pollution, and improper dosage may also cause tissue damage
In addition, the subcutaneous injection of platelet-rich plasma PRP gel is painful and the filling effect is not long-lasting

Method used

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  • Preparation method of autologous platelet-factor-rich plasma (PFRP) preparation
  • Preparation method of autologous platelet-factor-rich plasma (PFRP) preparation
  • Preparation method of autologous platelet-factor-rich plasma (PFRP) preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of autologous platelet-rich plasma PRP, collect 20ml of autologous peripheral anticoagulant blood, centrifuge within 2 hours after blood collection, centrifugal force 1800g, centrifugation time is 8min; discard the upper platelet-poor plasma and lower red blood cells after centrifugation, and retain the middle autologous platelet-rich plasma Plasma PRP (platelet-poor plasma in the upper section, platelet-rich plasma in the middle section, and red blood cells in the lower section have different colors, so they can be distinguished by color). ;

[0044] 2. Preparation of autologous platelet-rich factor plasma PFRP. Take out the autologous platelet-rich plasma PRP stored at -80°C, dissolve it at 37°C and centrifuge to obtain platelet-rich factor plasma PFRP for later use, and discard the bottom membrane fragments; the centrifugal force during centrifugation is 4000g, the centrifugation time is 10min, and the whole process is aseptically operated;

[0045] 3...

Embodiment 2

[0047] 1. Preparation of autologous platelet-rich factor plasma PRP, collect 20ml of autologous anticoagulant blood, centrifuge within 2 hours after blood collection, centrifugal force 1600g, centrifugation time is 10min; discard the upper platelet-poor plasma and lower red blood cells after centrifugation, and retain the middle autologous platelet-rich plasma Plasma PRP cryopreservation, aseptic operation throughout the process;

[0048] 2. Preparation of autologous platelet-rich factor plasma PFRP. Freeze-thaw the prepared autologous platelet-rich plasma PRP and centrifuge to obtain platelet-rich factor plasma PFRP for later use. Discard the bottom membrane fragments; the centrifugal force during the centrifugation process is 4000g, and the centrifugation time is 10min. Full aseptic operation;

[0049]3. Preparation of autologous platelet-rich factor plasma PFRP preparation. The obtained autologous platelet-rich factor plasma PFRP and sodium hyaluronate were mixed at a volum...

Embodiment 3

[0051] 1. Preparation of autologous platelet-rich plasma PRP, collect 20ml of autologous peripheral anticoagulant blood, centrifuge within 2 hours after blood collection, centrifugal force 1200g, centrifugation time is 10min; discard the upper platelet-poor plasma and lower red blood cells after centrifugation, and retain the middle autologous platelet-rich plasma Plasma PRP, the prepared autologous platelet-rich factor plasma PRP was stored at -80°C for later use, and the whole process was aseptically operated;

[0052] 2. Preparation of autologous platelet-rich factor plasma PFRP. The autologous platelet-rich plasma PRP stored at -80°C was taken out, melted at 37°C and centrifuged to obtain platelet-rich factor plasma PFRP for later use, and the bottom membrane fragments were discarded. The centrifugal force during the centrifugation process is 4000g, the centrifugation time is 10min, and the whole process is aseptically operated;

[0053] 3. Preparation of autologous platel...

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Abstract

The invention discloses a preparation method of an autologous platelet-factor-rich plasma (PFRP) preparation. The preparation method is characterized by comprising the steps of: A, preparation of autologous PFRP, to be specific, collecting autologous whole blood, and preparing the autologous PFRP in a centrifuging and freeze-thawing manner; and B, preparation of the PFRP preparation, to be specific, mixing the obtained PFRP with a biological scaffold / autologous somatic cells according to a volume ratio of (3:1)-(1:1) to obtain the PFRP preparation. By means of specially processing the autologous whole blood to obtain the autologous PFRP, pollution risks caused by adding an activating agent are reduced, and the immune risks of a membrane antigen during heterogenous usage are avoided. The autologous PFRP preparation prepared according to the method not only has a good effect of repairing damaged tissues such as meniscus, but also has very good anti-wrinkle, filling and lifting effects.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to an autologous blood factor-rich plasma PFRP preparation, in particular to a preparation method of an autologous blood factor-rich plasma PFRP (Platele-factor-rich plasma) preparation. Background technique [0002] In clinical research, it has been reported that platelet-rich plasma PRP (Plate-rich plasma) gel is used to treat meniscus injury. The preparation of platelet-rich plasma PRP gel requires adding thrombin and calcium ions to platelet-rich plasma PRP. Thrombin is the most effective component of platelet activators. The use of animal-derived thrombin and calcium ions may lead to exogenous pollution, and improper dosage may also cause tissue damage. In addition, subcutaneous injection of platelet-rich plasma PRP gel is painful and the filling effect is not long-lasting. Contents of the invention [0003] The purpose of the present invention is to provide an innovative ...

Claims

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Application Information

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IPC IPC(8): A61L31/16A61L31/06A61L31/04A61L31/02
Inventor 陈静娴申重阳海泉胡忠国赵令卉王翔王小燕郑榆坤赵峻
Owner CHENGDU QINGKE BIOTECH
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