Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

2-furoacridone-beta-dihydroagarofuran sesquiterpene compound in leafy parnassia, and preparation method and application thereof

A technology of dihydro agarwood furans and sesquiterpenes, which is applied in the field of medicine, can solve the research on the anti-tumor activity of 2-keto-β-dihydro agarwood furans compounds with complex and diverse structure types, complex and diverse substitution types, and 2-keto-β-dihydro agarwood furans. Issues that have not been reported

Inactive Publication Date: 2013-05-01
NORTHWEST A & F UNIV
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the parent structure of β-dihydroagarfuran compounds is simple, the number of substituents varies from 2 to 9, and the substitution types are complex and diverse, so the structure types of these compounds are complex and diverse
Most of the existing β-dihydroagarwood furan compounds with insecticidal and anti-tumor activities have a 4-β hydroxyl group as the core structure, while 2-keto-β-dihydroagarwood furan compounds have a rare structure, and have No report on the anti-anti-tumor activity of 2-keto-β-dihydroagarfuran compounds

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 2-furoacridone-beta-dihydroagarofuran sesquiterpene compound in leafy parnassia, and preparation method and application thereof
  • 2-furoacridone-beta-dihydroagarofuran sesquiterpene compound in leafy parnassia, and preparation method and application thereof
  • 2-furoacridone-beta-dihydroagarofuran sesquiterpene compound in leafy parnassia, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Compound preparation

[0020] 1. Extraction

[0021] The dry weight of the whole herb of Xanthium chinensis is 1250g, and it is extracted with 95% ethanol at room temperature for 5 times, each time for 12 hours. After rotary evaporation to dryness, the ethanol phase extract was obtained, then suspended in water, followed by extraction with petroleum ether, ethyl acetate, and n-butanol to obtain 36.43 g of petroleum ether phase, 9.52 g of ethyl acetate phase, and 13.00 g of n-butanol phase. g and the aqueous phase 35.37 g.

[0022] Two, rough classification

[0023] After the ethyl acetate extract of cocklebur heptaacetate was suspended in water, it was extracted with petroleum ether, ether, ethyl acetate and n-butanol respectively to obtain C-E-1 (petroleum ether extraction layer), C-E-2 (ether extraction layer), C-E- 3 (ethyl acetate extraction layer, 5 g), C-E-4 (n-butanol extraction layer).

[0024] 3. Separation

[0025] Take 5 g of C-E-3 extract, dissolve with...

Embodiment 2

[0039] In vitro anti-tumor experiment

[0040] 1. Determination of tumor cell inhibition rate: MTT method

[0041] Tested cell lines: Two cell lines of human liver cancer cell HepG2 and human breast cancer cell line MDA-10 were selected.

[0042] Operation steps: Inoculate 2.5×10 per well in a 96-well plate 4 Each cell was incubated in a carbon dioxide cell incubator at 37°C for 6 hours, the culture solution in each well was sucked off, monomer compounds were added and cultured for 72 hours, stained with methylene blue staining, and the absorbance value was detected with a microplate reader. Investigate and compare the inhibitory rate of monomeric compounds on tumor cell proliferation.

[0043] 2. Test results

[0044] Table 2 Compounds inhibit the IC of different tumor cell proliferation 50 Value (μM)

[0045] Test sample

[0046] The test results showed that (1S, 4R, 5S, 6R, 7R, 8S, 9S, 10R)-1β-acetoxy-6β, 9β-dibenzoyloxy-2-keto-8β-hydroxyl-β- Dihydro agarwoo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses 2-furoacridone-beta-dihydroagarofuran sesquiterpene compound in leafy parnassia, and a preparation method and the application thereof. The method concretely comprises the step that a new beta-dihydroagarofuran sesquiterpene compound is separated from leafy parnassia plant in family saxifragaceae, with the molecular formula being C31H34O9. Modern spectral analysis determines the chemical structure and the spatial configuration of the compound, and the compound is named (1S, 4R, 5S, 6R, 7R, 8S, 9S, 10R)-1beta-acetoxyl-6beta, 9beta-diphenyl methanoyl-2-keto-8beta-oxhydryl-beta-dihydroagarofuran. In vitro antitumor test shows that the compound has obvious inbibitional effect on the proliferation of HepG2 human body hepatoma carcinoma cells and MDA-10 human body breast cancer cells. The compound can provide lead compound for researching new antineoplastic drugs, and provide an important theoretical basis for developing and utilizing a new plant origin--leafy parnassia plant in family saxifragaceae of beta-dihydroagarofuran sesquiterpene compound.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an anti-tumor compound, in particular to a new 2-keto-β-dihydroagarfuran sesquiterpene compound from Xanthium chinensis and its preparation method and application. Background technique [0002] Malignant tumor is a disease that seriously endangers human life. Its mortality rate is second only to cardiovascular and cerebrovascular diseases, and its incidence is increasing year by year. The study of anticancer drugs has been the focus and focus of the world's pharmaceutical research for many years. The clinical application of anti-tumor chemical drugs is greatly limited due to their high toxicity and side effects. Therefore, finding new anti-tumor active drugs from natural products has attracted more and more attention in recent years. [0003] At present, natural β-dihydroagarfuran compounds are mainly obtained from Euonymus plants. In recent decades, hundreds of β-dihydroagarfur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/94A61K31/343A61P35/00A61P1/16A61P15/14
Inventor 王冬梅李登武浦文君刘建军
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com