Fish skin and fish scale protein enzymolysis powder with function of relieving fatigue

Abstract

The invention discloses fish skin and fish scale protein enzymolysis powder with a function of relieving fatigue. Every 100g of fish skin and fish scale protein enzymolysis powder comprises the following components by weight: 80g-85g of protein, 3g-4g of glycogen and 10g of L-hydroxyproline. The preparation method for the fish skin and fish scale protein enzymolysis powder comprises the following steps of: removing fishy smell and color of enzymatic hydrolysate by utilizing active carbon after carrying out enzymolysis of the fish skin and the fish scales; and carrying out vacuum concentration and vacuum drying to obtain protein enzymolysis powder. The fish skin and fish scale protein enzymolysis powder prepared by the preparation method can be used for supplementing a lot of substances such as amino acid consumed by the human body after being eaten, and used for reliving a lot of accumulated fatigue factors such as lactic acid, uric acid, urea and oxygen radical in the human body to quickly relieve the fatigue.

Description

technical field

[0001] The invention relates to an enzymatic hydrolyzed powder of fish skin and fish scale protein, in particular to an enzymatic hydrolyzed powder of fish skin and fish scale protein with the function of relieving fatigue, and belongs to the technical field of health-care functional food production. Background technique

[0002] At present, chronic fatigue syndrome caused by various reasons such as increased work and life pressure affects the health of the human body and has become a social problem that people pay attention to. Therefore, there is an urgent need to develop a functional food that can relieve fatigue quickly. But at present, most fatigue-relieving products on the market are mostly formulated with traditional Chinese medicines such as Codonopsis pilosula and other extracts or taurine, and there are no fish skin and fish scale protein products that use fish processing waste.

[0003] A large amount of fish skin and scales are left in the proces...

Examples

Embodiment 1

[0021] Tilapia scales are used as raw materials, and the protease used to prepare the enzymolysis solution is Bacillus subtilis neutral protease, which is decolorized by activated carbon and dried in vacuum. The process steps are:

[0022] (1) Enzymatic hydrolysis of fish scales: take 5000g decalcified fish scales, add water according to the ratio of material to water 1:5, add Bacillus subtilis neutral protease at a ratio of 10000U / g fish scale weight, hydrolyze at 63°C for 3 hours, inactivate the enzyme at 95°C for 10min, cool , centrifuge (4000rpm, 30min), take the supernatant;

[0023] (2) Deodorization and decolorization of the enzymatic hydrolysis solution: add 1% activated carbon according to the mass percentage of the enzymatic hydrolysis solution, incubate at 50°C for 20 minutes, and filter with diatomaceous earth to obtain a clarified fish scale enzymatic hydrolysis solution;

[0024] (3) Vacuum concentration of the enzymolysis solution: Concentrate the clarified fis...

Embodiment 2

[0027] Tilapia scales are used as raw materials, and the protease used to prepare the enzymolysis solution is Bacillus subtilis neutral protease, which is decolorized by activated carbon and dried in vacuum. The process steps are:

[0028] (1) Enzymatic hydrolysis of fish scales: Take 5000g decalcified fish scales and add water according to the ratio of material to water 1:5, then add neutral protease and papain to compound according to the weight ratio of 15000U / g fish scales (the ratio is 1:1), hydrolyze at 65℃ for 4 hour, inactivate the enzyme at 100°C for 20 minutes, cool down, centrifuge (4000rpm, 30min), and take the supernatant;

[0029] (2) Deodorization and decolorization of the enzymatic hydrolysis solution: add 2% activated carbon according to the mass percentage of the enzymatic hydrolysis solution, keep warm at 60°C for 40 minutes, and filter with diatomaceous earth to obtain a clarified fish scale enzymatic hydrolysis solution;

[0030] (3) Vacuum concentration ...

Embodiment 3

[0033] Using tilapia skin as raw material, the protease used in the preparation of the enzymatic hydrolyzate is neutral protease: papain is compounded at a ratio of 1:1, decolorized by activated carbon, and freeze-dried. The process steps are:

[0034] (1) Fat removal from fish skin: Take 5000g fish skin and add water according to the material-to-water ratio of 1:2, then heat and stir to 95°C, pick up, wash with running water, and drain to remove fat;

[0035] (2) Fish skin enzymatic hydrolysis: add water according to the weight of fish skin at 1:1, add 10000U / g fish skin weight compound protease, enzymolyze at 55°C for 4h, inactivate enzyme at 95°C for 10min, cool, and centrifuge (4000rpm, almost 30min), take the supernatant;

[0036] (3) Vacuum concentration of the enzymolysis solution: Concentrate the clarified fish scale enzymolysis solution at 60°C and -0.085MPa to a soluble solid content of 18-20%;

[0037] (4) Vacuum drying: vacuum-dry the concentrated solution at 65°...

technical field

[0001] The invention relates to an enzymatic hydrolyzed powder of fish skin and fish scale protein, in particular to an enzymatic hydrolyzed powder of fish skin and fish scale protein with the function of relieving fatigue, and belongs to the technical field of health-care functional food production. Background technique

[0002] At present, chronic fatigue syndrome caused by various reasons such as increased work and life pressure affects the health of the human body and has become a social problem that people pay attention to. Therefore, there is an urgent need to develop a functional food that can relieve fatigue quickly. But at present, most fatigue-relieving products on the market are mostly formulated with traditional Chinese medicines such as Codonopsis pilosula and other extracts or taurine, and there are no fish skin and fish scale protein products that use fish processing waste.

[0003] A large amount of fish skin and scales are left in the proces...

Examples

Embodiment 1

[0021] Tilapia scales are used as raw materials, and the protease used to prepare the enzymolysis solution is Bacillus subtilis neutral protease, which is decolorized by activated carbon and dried in vacuum. The process steps are:

[0022] (1) Enzymatic hydrolysis of fish scales: take 5000g decalcified fish scales, add water according to the ratio of material to water 1:5, add Bacillus subtilis neutral protease at a ratio of 10000U / g fish scale weight, hydrolyze at 63°C for 3 hours, inactivate the enzyme at 95°C for 10min, cool , centrifuge (4000rpm, 30min), take the supernatant;

[0023] (2) Deodorization and decolorization of the enzymatic hydrolysis solution: add 1% activated carbon according to the mass percentage of the enzymatic hydrolysis solution, incubate at 50°C for 20 minutes, and filter with diatomaceous earth to obtain a clarified fish scale enzymatic hydrolysis solution;

[0024] (3) Vacuum concentration of the enzymolysis solution: Concentrate the clarified fis...

Embodiment 2

[0027] Tilapia scales are used as raw materials, and the protease used to prepare the enzymolysis solution is Bacillus subtilis neutral protease, which is decolorized by activated carbon and dried in vacuum. The process steps are:

[0028] (1) Enzymatic hydrolysis of fish scales: Take 5000g decalcified fish scales and add water according to the ratio of material to water 1:5, then add neutral protease and papain to compound according to the weight ratio of 15000U / g fish scales (the ratio is 1:1), hydrolyze at 65℃ for 4 hour, inactivate the enzyme at 100°C for 20 minutes, cool down, centrifuge (4000rpm, 30min), and take the supernatant;

[0029] (2) Deodorization and decolorization of the enzymatic hydrolysis solution: add 2% activated carbon according to the mass percentage of the enzymatic hydrolysis solution, keep warm at 60°C for 40 minutes, and filter with diatomaceous earth to obtain a clarified fish scale enzymatic hydrolysis solution;

[0030] (3) Vacuum concentration ...

Embodiment 3

[0033] Using tilapia skin as raw material, the protease used in the preparation of the enzymatic hydrolyzate is neutral protease: papain is compounded at a ratio of 1:1, decolorized by activated carbon, and freeze-dried. The process steps are:

[0034] (1) Fat removal from fish skin: Take 5000g fish skin and add water according to the material-to-water ratio of 1:2, then heat and stir to 95°C, pick up, wash with running water, and drain to remove fat;

[0035] (2) Fish skin enzymatic hydrolysis: add water according to the weight of fish skin at 1:1, add 10000U / g fish skin weight compound protease, enzymolyze at 55°C for 4h, inactivate enzyme at 95°C for 10min, cool, and centrifuge (4000rpm, almost 30min), take the supernatant;

[0036] (3) Vacuum concentration of the enzymolysis solution: Concentrate the clarified fish scale enzymolysis solution at 60°C and -0.085MPa to a soluble solid content of 18-20%;

[0037] (4) Vacuum drying: vacuum-dry the concentrated solution at 65°...

Images