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Optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, preparation process and application

A Bacillus licheniformis, preparation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., to achieve the effects of high number of viable bacteria, optimization of preparation process, and increase of production cost

Inactive Publication Date: 2013-05-08
刘显军
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on Bacillus licheniformis-xylooligosaccharide synbiotic products on the market, so the research on the preparation parameters and process of Bacillus licheniformis-xylooligosaccharide synbiotics is of great significance to the production and application of synbiotics

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  • Optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, preparation process and application
  • Optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, preparation process and application
  • Optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, preparation process and application

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Embodiment Construction

[0028] (1) Drawing of Bacillus licheniformis growth curve and determination of culture conditions

[0029] In a sterile laboratory, inoculate the activated Bacillus licheniformis solution in a 100 mL conical flask ( Composition and ratio of LB medium: peptone 10.0g, yeast powder 5.0g, NaCl 10.0g, agar 15.0g, distilled water 1.0L, PH7.0), respectively, at 34℃-120r·min -1 , 37℃-120r·min -1 , 40℃-120r·min -1 and 34℃-150r·min -1 , 37℃-150r·min -1 ,40℃-150r·min -1 and 34℃-180r·min -1 , 37℃-180r·min -1 ,40℃-180r·min -1 Under the condition of shaking culture, take samples every 0.5h, culture continuously for 12h, measure its absorbance at 600nm wavelength, draw a curve with time (h) as abscissa and absorbance (OD value) as ordinate (see figure 1 , 2 , 3), according to the growth curve to determine the optimal temperature and speed of Bacillus licheniformis culture. Depend onfigure 1 It can be seen that the strain can grow well at three temperatures and speeds, but at 40°C, ...

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Abstract

The invention relates to an optimal selection method of preparation parameters of bacillus licheniformis-xylo-oligosaccharide synbiotics, a preparation process and an application. The method comprises the following steps of: inoculating bacterial liquid of activated bacillus licheniformis CICC20446 into a liquid culture medium containing 2.5-4 mg.mL<1> of xylo-oligosaccharide according to the capacity ratio of 1%, and placing the mixture in an incubator for shake cultivation for 3.5-4.5h under the conditions of 40 DEG C and 120 r.min<1>. The bacillus licheniformis-xylo-oligosaccharide synbiotics provided by the invention can be capable of obviously improving the daily gain of animals and reducing material-to-weight ratio and diarrhea rate, and has an obvious improvement effect on the growth of the animals.

Description

technical field [0001] The invention belongs to a micro-ecological regulator for animal feed, in particular to a method for optimizing the preparation parameters of a Bacillus licheniformis-xylo-oligosaccharide synbiotic, a preparation process and an application. Background technique [0002] The gut is the largest digestive organ in the body and also the largest immune organ. A stable intestinal microbiota can promote the improvement of intestinal function, and can improve the production performance and immune performance of animals. Under the stimulation of various external or internal factors (such as disease, weaning, etc.), the structure of intestinal microbial flora changes, harmful flora (such as Escherichia coli) increases, and beneficial flora (such as Lactobacillus, Bacillus ) will be reduced, which will lead to intestinal dysfunction, diarrhea, poor appetite, food allergies, decreased resistance, and repeated illnesses. For a long time, the prevention and treatm...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12R1/10
Inventor 刘显军李建涛陈静王佳
Owner 刘显军