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Application of poly cytosine binding protein-1 in preparation of medicine for preventing and treating Parkinson disease

A polycytosine and protein-binding technology that can be used in drug combinations, peptide/protein components, and pharmaceutical formulations to solve problems such as oxidative stress and cell damage

Inactive Publication Date: 2013-05-08
首都医科大学附属复兴医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We can hypothesize that, even if the total iron content in the cell does not change, the dysfunction or misfolding of the iron-storing ferritin under the action of the toxin will cause excess iron to be released, thereby triggering oxidative stress, leading to cell damage

Method used

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  • Application of poly cytosine binding protein-1 in preparation of medicine for preventing and treating Parkinson disease
  • Application of poly cytosine binding protein-1 in preparation of medicine for preventing and treating Parkinson disease
  • Application of poly cytosine binding protein-1 in preparation of medicine for preventing and treating Parkinson disease

Examples

Experimental program
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Embodiment 1

[0057] Example 1 Application of PCBP1 in preventing or resisting Parkinson's disease

[0058] 1 material

[0059] SH-SY5Y cells, purchased from American Standard Biology Collection (ATCC, CRL-2266); recombinant plasmid PCBP1-pcDNA containing hnRNPE1 sequence TM 4 / His C (constructed and preserved in our laboratory); plasmid pEGFP-N2 (GeneBank Accession#:U57608) was purchased from Invitrogen; the open reading frame (ORF) of human PCBP 1 cDNA (nt 292-1362) (GenBank accession#genbank: NM 006196) was cloned and recombined to form a PCBP1-pEGFP-N2 expression vector, and the specific sequence of PCBP1-ORF is shown in SEQ ID NO.1 in the sequence table.

[0060] Reagents used: TRIZOL reagent was purchased from Invitrogen; transfection reagent: lipofectamine TM 2000 was purchased from Invitrogen; PCR reagents and endonucleases were purchased from TaKaRa; various cell culture media were purchased from Gibicol; antibodies including PCBP1 and BDNF primary antibodies and their correspond...

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Abstract

The invention relates to application of poly cytosine binding protein-1 (PCBP1) in preparation of a medicine for preventing and treating Parkinson disease (PD). According to the construction of a cell model of the PD, the PCBP1 which is introduced from outside can be used for promoting the growth of the human dopaminergic cells (SH-SY5Y cells); simultaneously, the outside PCBP1 can inhibit the damage of the nerve cells caused by a PD toxic substance 6-OHDA; as the confocal analysis shown, the PCBP1 can improve the expression level of BDNF (Brain Derived Neurotrophic Factor) in PD model cells; in addition, pre-pouring the PCBP1 to improve the action of an animal disease model based on the animal model subjected to the PD further shows the effects of the isleta myloid poly peptide on preventing and resisting the PD, so that the multi-target isleta myloid poly peptide of the PCBP1 and similar isleta myloid poly peptide are expected to be developed into polypeptide biological preparations with the effect of resisting the PD, and the important value and the application prospect are brought in clinical application.

Description

technical field [0001] The present invention relates to a new application of polycytosine binding protein 1 (PCBP1), in particular to the application of polycytosine binding protein 1 (PCBP1) in the preparation of drugs for the prevention and treatment of Parkinson's disease, which belongs to the field of biomedicine . Background technique [0002] Parkinson's disease (PD) is a relatively common degenerative disease of the central nervous system, which mainly occurs in middle-aged and elderly people, and has the characteristics of high morbidity and disability. The main clinical symptoms of the disease are decreased voluntary movement, muscle stiffness, limb tremor, and postural balance disorder; the progressive degeneration of dopaminergic neurons in the substantia nigra compact part of the midbrain reduces the content of dopamine (DA) in the striatum, accompanied by The formation of Lewy bodies is the main pathological feature. According to the "Ninth Five-Year Plan" sur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P25/16A61P25/00
Inventor 霍丽蓉王晓民王兰英梁建涛
Owner 首都医科大学附属复兴医院
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