Quality detecting method of alpinia zerumbet medicinal material

A quality detection method and technology for medicinal materials, which are applied in measuring devices, material separation, and analysis of materials, etc., can solve the problem that the quality of Yanshan ginger medicinal materials cannot be effectively controlled, and achieve quality control, good recovery rate, and high accuracy and sensitivity. Effect

Inactive Publication Date: 2013-05-08
贵州远程制药有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Yanshanjiang is now included in the "Standards of Traditional Chinese Medicine and Ethnic Medicine in Guizhou Province" (2003 edition). The standard only includes the identification of the properties of the medicinal materials. Therefore, the quality of Yanshanjiang medicinal materials cannot be effectively controlled.

Method used

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  • Quality detecting method of alpinia zerumbet medicinal material
  • Quality detecting method of alpinia zerumbet medicinal material
  • Quality detecting method of alpinia zerumbet medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A quality detection method for the medicinal material of Yanshan Ginger, comprising the following steps:

[0025] (1) Preparation of the test solution: Accurately weigh 0.5g of medicinal material powder (passed through a 60-mesh sieve), accurately add 30mL of methanol, accurately weigh, extract in a water bath at 80°C for 2 hours, take it out and let it cool, and accurately weigh , use methanol to make up for weight loss, shake well, and filter through a microporous membrane (0.45 μm) to get final product;

[0026] Weigh two parallel samples S 1 , S 2 :

[0027]

[0028] (2) Preparation of reference substance solution: Accurately weigh 5.1 mg of demethoxycapsaicin reference substance dried to constant weight by phosphorus pentoxide, put it in a 25mL volumetric flask, add methanol to dissolve and dilute to the mark, shake well, and prepare into a reference solution containing 0.204 mg of demethoxycapsaicin per 1 mL. Dilute the above stock solution solution to a re...

Embodiment 2

[0040] A quality detection method for the medicinal material of Yanshan Ginger, comprising the following steps:

[0041] (1) Preparation of the test solution: Accurately weigh 0.5g of medicinal material powder (passed through a 60-mesh sieve), accurately add 30mL of methanol, accurately weigh, extract in a water bath at 80°C for 2 hours, take it out and let it cool, and accurately weigh , use methanol to make up for the weight loss, shake well, and filter through a microporous membrane (0.45 μm). ;

[0042] Weigh two parallel samples S 1 , S 2 :

[0043]

[0044] All the other are the same as embodiment 1

[0045] The measurement results:

[0046] sample injection Reference solution sample 1 sample 2 retention time (min) 12.73 12.70 12.76 Peak area 1528.7 1120.5 1132.1

[0047] ②Calculation: (average content 3.0270g / bag)

[0048]

Embodiment 3-12

[0050] Embodiment 3-12 ten batches of test sample measurement results are shown in the following table (method is the same as embodiment 1):

[0051] Example Batch Demethoxycapsaicin content (mg / g) 3 201107255 5.42 4 201107262 5.01 5 201107261 9.60 6 201107254 5.81 7 201107234 6.65 8 201107231 5.32 9 201108102 5.55 10 201108042 6.34 11 201108092 9.02 12 201108091 5.05

[0052] According to the content measurement results of 12 batches of samples in the above examples 1-12, and considering the difference in the place of origin of the medicinal materials, the picking period and the texture, it is determined that the content of demethoxycapsaicin in this medicinal material should not be less than 5 mg / g (calculated as dry product) ).

[0053]

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Abstract

The invention discloses a quality detecting method of an alpinia zerumbet medicinal material. The quality detecting method comprises the following steps of: preparation of a test solution and preparation of a comparison solution, wherein the chromatographic conditions are as follows: a chromatographic column is an AgilentEclipseXDB-C18 column (150mm*4.6mm, 5 microns); a volume ratio of (flowing phases) water to acetonitrile equals to 80:20; a flow rate is 1mL / min; the detecting wavelength is 345nm, the column temperature is 25 DEG C; and the feeding quantity is 10 microliters. A content determination method comprises the steps of: respectively and accurately absorbing 10 microliters of comparison solution and 10 microliters of test solution, injecting the comparison solution and the test solution into a liquid chromatograph, and determining the solutions according to a high performance liquid chromatography, wherein the content of demethoxyyangonin is not less than 5mg / g by taking anhydrous substance as a reference. The quality detecting method of the alpinia zerumbet medicinal material is high in accuracy, reliable, easy to operate, good in reproducibility, and capable of effectively controlling the quality of the alpinia zerumbet medicinal material.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a method for detecting the quality of Alpinia japonicus medicinal material. [0002] Background technique [0003] Yanshanjiang is a plant of Zingiberaceae Yanshanjiang ( Alpinia zerumbet (Pers.) Burtt. et Smith ) fresh or dried ripe fruit. [0004] Properties: This product is spherical, slightly pointed at both ends, about 2cm long, 1.5cm in diameter, yellowish brown, slightly shiny, with more than ten raised longitudinal ribs, with a protrusion at the top, which is the remnant of perianth, and some at the base With fruit stalk broken marks. The petals of the seed clusters are loosely arranged, easy to scatter, and the aril is membranous and white. The seeds are polyhedrons, 0.4-0.5 cm long and 0.3-0.4 cm in diameter. Slight gas, light taste, slightly pungent. [0005] Identification: [0006] ① Seed cross-section: epidermis cells of test...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 黄家宇李莉谭济苍
Owner 贵州远程制药有限责任公司
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