Kit and method for detecting porous dental hydroxy apatite

A hydroxyapatite and kit technology, which can be used in the detection/measurement of irregular teeth, dental preparations, dentistry, etc., can solve the problems of difficult differential diagnosis and poor MIH effect.

Active Publication Date: 2013-05-08
INCISIVE TECH PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are currently no products available for the diagnosis and restoration of MIH or other DDD, and the differential diagnosis of caries and different types of DDD is diffi

Method used

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  • Kit and method for detecting porous dental hydroxy apatite
  • Kit and method for detecting porous dental hydroxy apatite
  • Kit and method for detecting porous dental hydroxy apatite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0185] Example 2 - Preparation and testing of porous hydroxyapatite probes

[0186] Materials and Methods

[0187] SMCC (N-hydroxysuccinimidyl succinimidyl 4-[N-maleimidomethyl]cyclohexanecarboxylate; CAS#: 64987-85-5) is a non-cleavable isoform Bifunctional cross-linking agent, has been The spacer arm reactively separates the amine and sulfhydryl groups. Amino Black (CAS#: 1064-48-8) is a general purpose blue / black dye, used here as a chromogenic reporter containing primary amine groups. Hemoglobin from cow (CAS#: 9008-02-0) comprises a heterotetramer of 2 pairs of polypeptide chains (α and β; respectively: SEQ ID NO 20 and SEQ ID NO 21). The beta chain has a single solvent-exposed thiol-containing cysteine ​​residue, while the alpha chain has no cysteine.

[0188] SMCC (75 mM in dimethyl sulfoxide) was added to 9 volumes of amino black (37.5 mM in phosphate buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 10 mM disodium hydrogen phosphate, pH 7.4)) and Incubate at 21°C...

Embodiment 3

[0195] Example 3 - Probes that specifically bind to porous enamel ( Figure 30 )

[0196] method

[0197] To test whether the probe in Example 2 specifically binds to porous enamel, a complex carious lesion was covered with the probe and rinsed thoroughly.

[0198] Photograph of a human first molar with a large area of ​​caries (porous enamel, white opaque areas) before and after application of the probe ( Figure 30 ). Use a brush to apply the probe to the entire crown area for 1 minute. After application of the probe, the tooth was rinsed under running water for 10 seconds, photographed, and then the tooth was rinsed again for 2 minutes, and photographed.

[0199] result

[0200] Normal enamel is not labeled.

[0201] Clearly carious areas were marked specifically and clearly, but the markings were patchy in some places. Unmarked carious areas show a bright surface that is resistant to scratching, while marked areas have a dark surface that is easily scratched. T...

Embodiment 4

[0204] Example 4 - The probe is able to specifically detect early demineralization of the enamel surface (incipient caries model) ( Figure 31 )

[0205] method

[0206] To test whether the probe in Example 2 could specifically detect early caries, artificial caries lesions were created (before probe application) on normal enamel surfaces using strong acid spots.

[0207] Human first molars, caries-free prior to acid treatment, were photographed before and after probe application ( Figure 31 ). Three enamel areas were then exposed to acid (0.5 μl 85% H 3 PO 4 ) for 1, 3 or 10 minutes to introduce artificial carious lesions, followed by a two-minute rinse in 100 ml TBS (25 mM Tris pH 7.2, 160 mM NaCl), followed by an additional 2-minute rinse under running water. The tooth was air dried and the probe was applied to the entire area with a brush for 3 minutes. After application, unbound probes were first removed by wiping with absorbent paper and then rinsed under runnin...

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Abstract

The present invention relates to a kit and a probe for detecting porous dental hydroxyapatite, comprising a protein capable of binding porous dental hydroxyapatite or a detector thereof. The invention also relates to a method for detecting a condition involving porous dental hydroxyapatite comprising detecting in or on a tooth or a sample of the tooth of a subject a protein bound to porous dental hydroxyapatite. The invention also relates to methods for detecting a hypomineralisation developmental dental defect or detecting intact and/or broken MIH enamel, and to a kit and method for removing a protein bound to porous dental hydroxyapatite.

Description

technical field [0001] The present invention relates to a kit and a probe for detecting porous hydroxyapatite in teeth, and a method for detecting conditions involving porous hydroxyapatite in teeth. Background technique [0002] The elasticity of teeth depends on a complex interplay between minerals (called hydroxyapatite) and organic components (proteins, cells and tissues). Under normal conditions, hydroxyapatite in enamel and dentin forms an extremely dense structure that provides the hardness and toughness needed to maintain tooth integrity. The loss of mineral density in enamel and dentin leads to abnormally porous hydroxyapatite, which in turn leads to a decrease in tooth physical elasticity and structural damage. Porous hydroxyapatite is caused by several prevalent conditions, including dental caries and developmental tooth defects (DDD). [0003] Dental caries (tooth decay) is a disease caused by acid-secreting bacteria. Acids produced by cariogenic bacteria can ...

Claims

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Application Information

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IPC IPC(8): A61K6/00A61K49/00A61Q11/00
CPCG01N33/6893A61K49/0438C07K14/00A61K6/002A61K49/001A61K6/25A61K6/00A61K49/00A61Q11/00A61B5/4547A61K49/006
Inventor 迈克尔·詹姆斯·哈伯德乔纳森·爱德华·曼格姆
Owner INCISIVE TECH PTY LTD
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