Method for carrying out field rapid assistant screening on crop drought-resisting mutants

Abstract

The invention provides a method for carrying out field rapid assistant screening on crop drought-resisting mutants. The method comprises the following steps of: 1, acquiring a thermal infrared image; 2, extracting temperature distribution characteristic parameters, namely processing the acquired thermal infrared image; 3, screening a characteristic parameter index, namely screening a character parameter expressing a crop drought-resisting difference from the characteristic parameters extracted in the step 2; 4, determining a threshold; and 5, calculating the drought-resisting level of a crop to be screened, namely checking a drought-resisting level interval corresponding to the parameter obtained in the step 3, and determining the drought-resisting level of the mutants. According to the invention, the thermal-infrared-imaging-based method for screening the crop drought-resisting mutants is established on the basis of the thermal infrared imaging technology. The method can realize rapid and lossless screening in the field. Compared with a conventional screening method of crop drought-resisting mutants, the method has the advantages of convenience in system carrying, simplicity in operation, short measurement period, low detection cost and high identification precision.

Description

technical field [0001] The invention belongs to the field of crop breeding methods and technical means, and in particular relates to a method for selecting drought-resistant breeding materials. Background technique [0002] With global warming, drought and water shortage are becoming more and more severe, and drought has become one of the main environmental factors limiting crop production. At present, the world's arid areas account for about 36% of the land area, accounting for 42.9% of the total cultivated land area, and other areas are often affected by seasonal drought or unpredictable irregular drought. According to statistics, the reduction in production caused by worldwide drought can exceed the sum of the reduction in production caused by other factors. In my country, arid and semi-arid areas account for 47% of the country's land area and 51% of the total cultivated land area. In the past 40 years, my country has lost a total of nearly 100 million tons of grain due...

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Problems solved by technology

[0008] There are also certain defects in the above-mentioned prior art: the field morphological identification method, which uses the external morphological parameters of crops as a feature to screen drought-resistant plants, can identify and evaluate the drought resistance of crops to a certain extent, but it needs to be tested in different Observation and determination are required for each period, which consumes a lot of manpower and material resources, and it needs to be determined in combination with other drought resistance indicators; in addition, this identification method mainly relies on artificial naked eye observation, which is relatively subjective, and the screening results of different breeders are different. larger;

[0009] Physiological and biochemical testing methods, which characterize drought resistance based on changes in biochemical components of crops under drought stress, need to be sampled and taken back to the laboratory for measurement using specific biochemical experimental instruments. The need for lossless screening;

[0010] Molecular marker method, using molecular technology to screen for drought resistance, the result is very accurate, but the operation is complicated, the cost is high, and it is difficult to screen a large number of materials;

[0011] Yield determination is a comprehensive, most widely used and relatively accurate identification method. It is currently mainly used in the drought resistance identification of high-generation strains or germplasm resources in breeding. For a large number of early-generation segregation in the breeding process Generation materials, difficult to apply in large quantities

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