Pharmaceutical composition, and preparation method and application thereof
A composition and drug technology, applied in the directions of drug combination, medical formula, plant raw material, etc., can solve the problem of no drug composition being researched, and achieve the effects of enhancing immunity, lowering body immunity, and improving insulin resistance.
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Embodiment 1
[0029] The raw material drug formula is:
[0030] 50g of white grass, 30g of astragalus, 60g of bitter gourd, 30g of Pu'er tea, 10g of dew grass.
[0031] Preparation:
[0032] a. Take the Chinese herbal medicine according to the weight ratio of the crude drug, wash and powder it into powder and pass through a 60-mesh sieve for subsequent use;
[0033] b. Combine white grass, bitter gourd, and Puer tea, add water to decoct twice, add 15 times the amount of water for the first time, decoct for 2 hours, add 8 times the amount of water for the second time, decoct for 1 hour, and combine the decoction , filtered, and the filtrate was concentrated to a thick paste with a relative density of 1.25-1.30 (60°C), dried at 68°C for later use;
[0034] c. Merge astragalus and dew grass, add 15 times the amount of 75% ethanol to soak for 24 hours, extract with 500W ultrasonic wave for 30 minutes, filter, concentrate the filtrate to a thick paste with a relative density of 1.25-1.30 (60°C...
Embodiment 2
[0038] The raw material drug formula is:
[0039] 60g of white grass, 20g of astragalus, 50g of bitter gourd, 40g of Pu'er tea, 10g of dew grass.
[0040] Preparation:
[0041] a. Take the Chinese herbal medicine according to the weight ratio of the crude drug, wash and powder it into powder and pass through a 60-mesh sieve for subsequent use;
[0042] b. Combine white grass, bitter gourd, and Puer tea, add water to decoct twice, add 15 times the amount of water for the first time, decoct for 2 hours, add 8 times the amount of water for the second time, decoct for 1 hour, and combine the decoction , filtered, and the filtrate was concentrated to a thick paste with a relative density of 1.25-1.30 (60°C), dried at 68°C for later use;
[0043] c. Merge astragalus and dew grass, add 15 times the amount of 75% ethanol to soak for 24 hours, extract with 500W ultrasonic wave for 30 minutes, filter, concentrate the filtrate to a thick paste with a relative density of 1.25-1.30 (60°C...
Embodiment 3
[0047] In Vitro Experiment of Improving Insulin Resistance by the Active Ingredients FL1, FL2, FL3 and FL4 of the Pharmaceutical Composition of the Present Invention
[0048] 1. Establishment of 3T3-L1 cell insulin resistance model
[0049]3T3-L1 cells were cultured on a 6-well plate with high-glucose DMEM medium. After the cells reached a certain density, they were replaced with a medium containing a certain concentration of insulin, dexamethasone, and IBMX for value-added culture. After a certain level, the cells were seeded in 24-well plates and cultured with normal medium for 2 days, and resistance was induced after 2 days. The 2 wells were used as blank control, and the rest were cultured with medium containing insulin and dexamethasone. After 5 days of induction, Detect the glucose concentration in the cell culture medium. When the glucose concentration that induces resistance reaches 0.5-1 times the glucose concentration of the blank control, the establishment of resist...
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