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Method for extracting peanut polysaccharide from cold pressed peanut cake

A technology of cold-pressing peanut meal and peanut meal, which is applied in the field of extracting peanut polysaccharides, can solve the problem of low purity of peanut meal polysaccharides, and achieve the effects of small structural damage, mild conditions, and improved purity

Active Publication Date: 2013-06-05
北京中农探味科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the purity of the peanut meal polysaccharide obtained by the above extraction method is low, because the peanut meal contains a large amount of protein, so in order to obtain a high-purity peanut meal polysaccharide, the protein in the peanut meal should be effectively removed.

Method used

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  • Method for extracting peanut polysaccharide from cold pressed peanut cake

Examples

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Effect test

Embodiment 1

[0037] Embodiment 1, prepare peanut polysaccharide from cold-pressed peanut meal

[0038] (1) Take cold-pressed peanut meal powder, add distilled water, and prepare a peanut meal suspension with a mass concentration of 8% to dissolve it, so as to fully combine with protease;

[0039] (2) Add Neutrase neutral protease (purchased from Beijing Suolaibao Technology Co., Ltd.) to the above solution, the enzyme dosage is 5200U / g cold-pressed peanut meal, and enzymatically react in a constant temperature water bath shaker at 45°C for 150min;

[0040] (3) Take it out after the enzymatic hydrolysis reaction, put it in a constant temperature water bath at 90°C for 10 minutes to inactivate neutral protease, and centrifuge at 4500r / min for 20 minutes to obtain a precipitate, which is the peanut meal residue from which protein has been removed;

[0041] (4) Extract polysaccharides from the obtained peanut meal residue by hot water extraction, the extraction temperature is 90°C, the extract...

Embodiment 2

[0045] Embodiment 2, the method for preparing peanut polysaccharide from cold-pressed peanut meal

[0046] (1) Take cold-pressed peanut meal powder, add distilled water, and prepare a peanut meal solution with a concentration of 8% to dissolve it, so as to fully combine with protease;

[0047] (2) Add Flavorzyme neutral protease (purchased from Beijing Suolaibao Technology Co., Ltd.) to the above solution, the enzyme dosage is 5200U / g cold-pressed peanut meal, and enzymatically react in a constant temperature water bath shaker at 45°C for 150min;

[0048] (3) Take it out after the reaction is over, put it in a constant temperature water bath at 90°C for 10 minutes to inactivate neutral protease, and centrifuge at 4500r / min for 20 minutes to obtain a precipitate, which is the peanut meal residue from which protein has been removed;

[0049] (4) Extract polysaccharides from the peanut meal residue obtained above by hot water extraction, the extraction temperature is 80°C, the ex...

Embodiment 3

[0053] Embodiment 3, the method for preparing peanut polysaccharide from cold-pressed peanut meal

[0054] (1) Take cold-pressed peanut meal powder, add distilled water, and prepare a peanut meal solution with a concentration of 8% to dissolve it, so as to fully combine with protease;

[0055] (2) Add Protamex neutral protease (purchased from Beijing Suolaibao Technology Co., Ltd.) to the above solution, the enzyme dosage is 2600U / g cold-pressed peanut meal, and react in a constant temperature water bath shaker at 45°C for 150min;

[0056] (3) Take it out after the reaction is over, put it in a constant temperature water bath at 90°C for 10 minutes to inactivate neutral protease, and centrifuge at 4500r / min for 20 minutes to obtain a precipitate, which is the peanut meal residue from which protein has been removed;

[0057] (4) Extract polysaccharides from the peanut meal residue obtained above by hot water extraction, the extraction temperature is 50°C, the extraction time is...

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Abstract

The invention discloses a method for extracting peanut polysaccharide from cold pressed peanut cake. The method comprises the following steps of: (1) adding water into cold pressed peanut cake powder to obtain a peanut cake suspension; (2) adding a neutral protease into the peanut cake suspension to carry out an enzymatic hydrolysis reaction; (3) after the enzymatic hydrolysis reaction is ended, carrying out enzyme inactivation treatment on a reaction liquid obtained by the enzymatic hydrolysis reaction, and then, separating the reaction liquid to obtain protein-removed peanut cake residues; (4) extracting the peanut polysaccharide from the protein-removed peanut cake residues by using hot water to obtain a peanut cake polysaccharide extracting solution, wherein the hot water extraction temperature ranges from 50 DEG C to 90 DEG C; and (5) adding ethanol into the supernatant liquid of the peanut cake polysaccharide extracting solution to carry out sedimentation treatment to obtain a precipitate, i.e., the peanut polysaccharide. The peanut cake is subjected to enzymatic hydrolysis by using an efficient and nontoxic biological enzyme to remove proteins in the peanut cake effectively, so that the purity of the peanut polysaccharide is increased and is as high as 92.87%, and the extracting rate of the peanut polysaccharide is up to 27.04%.

Description

technical field [0001] The invention relates to a method for extracting peanut polysaccharide from cold-pressed peanut meal. Background technique [0002] The output of my country's peanut planting area is increasing year by year, with a total annual output of about 13.341 million tons, accounting for more than 40% of the global total, ranking first in the world. 55% of the peanuts produced in my country are used for oil production, 30% are used for food, 7% are directly exported as peanut kernels, and 8% are used as seeds. Among them, the annual peanut cake after oil extraction is about 2.7 million tons. It contains a variety of active ingredients, among which polysaccharide is the second largest component of peanut meal, and the content of soluble sugar in peanut meal is 32.50%. Polysaccharides have biological activities such as immune regulation, anti-tumor, anti-oxidation, and hypolipidemic, but peanut meal polysaccharides have not been fully developed and utilized. At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王强刘红芝刘丽姜楠胡晖
Owner 北京中农探味科技有限公司
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