Method for analyzing Parkinson disease therapeutic effect
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A treatment effect and Parkinson's disease technology, applied in the field of detection and analysis, can solve problems affecting PD movement behavior, etc., and achieve the effect of friendly detection environment, mature equipment and instrument technology, and reliable principles
Inactive Publication Date: 2013-06-12
QINGDAO UNIV
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Long-term research and a large number of experimental results have shown that XE991 has a significant modulation effect on the electrical activity of dopaminergic neurons in the substantia nigra, and thus affects the motor behavior of PD. There are literature reports on its role in the prevention and treatment of PD and the detection method of its therapeutic effect, so it is of great social value and scientific significance to explore a detection and analysis method for the therapeutic effect of XE991 on Parkinson's disease
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Embodiment 1
[0023] The procedure for analyzing the effect of the KCNQ potassium channel blocker XE991 on the electrical activity of substantia nigra dopaminergic neurons involved in this example is as follows:
[0024] (1) Preliminary preparation: select rats 12 to 14 days after birth, place them at room temperature 20-24°C, 50-60% relative humidity, and live under 12:12h day and night cycle light conditions, free to drink and eat, Standby; XE991 is a product of Tocris Bioscience, dissolved in DMSO and stored at 40mmol / L, for use; in the experiment, it is diluted with artificial cerebrospinal fluid to the required concentration of 1μmol / L;
[0025] (2) Solution preparation: artificial cerebrospinal fluid (ACSF) (mmol / L): 124NaCl, 3KCl, 1.3NaH 2 PO 4 , 26NaHCO 3 , 2.4CaCl 2 , 1.3MgSO 4 , 10glucose, 5HEPES, 1mol / LNaOH to adjust the pH to 7.4, continuous use containing 5% CO 2 And 95%O 2 Oxygenation of the mixed gas; Electrode liquid (mmol / L): 120K-gluconate, 20KCl, 10HEPES, 1EGTA, 2MgCl2, 2Na2ATP...
Embodiment 2
[0041] This example relates to the analysis of the therapeutic effect of KCNQ potassium channel blocker XE991 on Parkinson's disease. The specific analysis process is:
[0042] (1) Condition preparation:
[0043] Choose adult rats, weighing 200g~250g, placed at room temperature 20~24°C, 50~60% relative humidity, living under 12:12h day and night light cycle, free drinking and eating, spare; choose medicine as haloperidine Alcohol (haloperidol), provided by American Sigma company; choose the experimental instrument as the animal brain stereotaxic instrument (SP-6N, Japan Narishige company product);
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Abstract
The invention belongs to the technical field of detection and analysis, and relates to a method for analyzing Parkinson disease therapeutic effects. The method comprises the following steps of: selecting adult rats and living under room temperature condition, and selecting haloperidol as the medicine; selecting an animal brain three-dimensional position finder; dividing the rats into two groups, namely, a reference group and an XE991 group; in the state that the rats are conscious and act freely, carrying out intraperitoneal injection of haloperidol, and subsequently injecting normal saline or XE991 into substantia nigra through a metal casing; observing whether the heads of the rats deflect or not and observing the deflection directions and the angles, and scoring according to the head-back straight lines of the rats and the included angles of the back-tail straight lines; slightly putting the front legs of the rats on a metal rod so as to record the rigidity incubation periods of the double front legs of the rats on the metal rods, wherein the test results show that the XE991 has the therapeutic effect on the PD (Parkinson's Disease) rigid rats induced by haloperidol, thereby realizing the analysis on the Parkinson's disease therapeutic effects. The method is simple in detection and analysis process and reliable in principle, the used equipment and instruments are developed in techniques, the detection data are accurate and the detection is environment-friendly.
Description
Technical field: [0001] The invention belongs to the technical field of detection and analysis, and relates to a method for judging the therapeutic effect of KCNQ potassium channel blocker XE991 on Parkinson's disease, in particular, the modulating effect of XE991 on dopaminergic neurons and an analysis method for its therapeutic effect on Parkinson's disease . Background technique: [0002] With the intensification of the population aging process in the whole society, neurodegenerative diseases have become one of the diseases that seriously endanger human health. Parkinson's disease (PD) is the second largest degenerative disease of the nervous system after Alzheimer's disease. The main pathological changes are the loss of substantia nigra dopaminergic neurons and the decrease of dopamine transmitter content, leading to quiescence in the patient Various clinical symptoms such as sexual tremor, muscle stiffness, impaired movement and postural reflex disorders. So far, although ...
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