Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compound, and preparation method and application thereof

A technology for separation and purification of compounds, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problem of few studies on metabolites, and achieve the effect of extensive development and application prospects

Inactive Publication Date: 2013-06-12
SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the metabolites of this strain

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound, and preparation method and application thereof
  • Compound, and preparation method and application thereof
  • Compound, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the preparation of compound

[0027] 1.1. Fermentation

[0028] The Xylaria sp. strain HCCB03890 was solidly fermented with a rice medium, the fermentation temperature was 27-30° C., and the fermentation time was 28-35 days.

[0029] 1.2. Separation and purification

[0030] The fermented rice product obtained in 1.1 was extracted with ethyl acetate, and then separated on a column with vacuum silica gel, eluted with ethyl acetate, and concentrated to obtain a crude sample.

[0031] Then, use Diane P680 liquid chromatography (preparative column YMC-ODS C-18, 5 μm, 20 × 250mm) to separate (0-40min with 20%-100% methanol elution, flow rate 8ml / min), to obtain crude Sample.

[0032] The obtained crude sample was further separated by semi-preparative liquid chromatography (the semi-preparative column was Agilent ZORBAX SB-C18, 5 μm, 9.4mm × 250mm) (0-25min with 18% acetonitrile isocratic elution, 25-45min with 18% ~35% acetonitrile gradient elution, flow ra...

Embodiment 2

[0033] Example 2 , Compound structure identification

[0034] The compound obtained in Example 1 is detected by positive ion electrospray mass spectrometry, and the spectrum is as follows figure 1 As shown, it shows that the quasi-molecular ion peak is: m / z 357.1186[M+H] + , the corresponding molecular formula is C 16 h 21 o 9 , adopt Bruker Avance II-400 type superconducting nuclear magnetic resonance instrument to measure the hydrogen spectrum of sample ( figure 2 ), carbon spectrum ( image 3 ) and NMR (MeOH-d 6 , 400MHz), and the NMR data are shown in Table 1.

[0035] Table 1. NMR data of compounds

[0036]

[0037]

[0038] Through analysis, the ownership of all carbon atoms and hydrogen atoms of the compound was determined, and the structure of the compound was obtained as follows:

[0039]

[0040] The molecular formula of this compound is C 16 h 20 o 9 , the molecular weight is 356, the chemical Chinese name is 2-methyl-7-(3,4,5-trihydroxy-6(hyd...

Embodiment 3

[0043] Embodiment 3, the biological activity of compound

[0044] The cells used in this example (all derived from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences, and the Cell Resource Center of the Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences) are: A549 (human non-small cell lung cancer cells), MDA-MB-231 ( human breast cancer cells), PANC-1 (human pancreatic cancer cells).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a compound with the following structural formula, and provides a method for preparing the compound by fermentation culture of a Xylaria sp. strain CGMCC No. 5410. The compound has good anti-tumor activity for a plurality of tumor cells such as human non-small cell lung cancer cells, human breast cancer cells, human pancreatic cancer cells and the like, and can be used for preparing anti-tumor drugs.

Description

technical field [0001] The present invention relates to new compounds, in particular, to a new compound and its preparation method and application. Background technique [0002] The inventors of the present application obtained a strain of Xylaria sp. through natural screening, named HCCB03890, and the preservation number of this strain is CGMCC No.5410. At present, there are few studies on the metabolites of this strain. Contents of the invention [0003] The inventors of the present application isolated a compound during the research on the metabolites of Xylaria sp. strain CGMCC No.5410, which was identified as a new compound by structure. [0004] Therefore, the primary object of the present invention is to provide a novel compound. [0005] The second object of the present invention is to provide the preparation method of said compound. [0006] The third object of the present invention is to provide the application of said compound. [0007] The compound of the p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H17/04C12P19/60A61P35/00C12R1/645
Inventor 杨志钧阮丽军戈梅魏维殷瑜
Owner SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com