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Method for utilizing molecularly imprinted electrochemical sensor to detect trace gibberellin A3

A technology of molecular imprinting and gibberellin, applied in the direction of electrochemical variables of materials, etc., can solve the problems such as the lack of relevant research on the detection of trace gibberellins, and achieve the effect of easy automation of detection, and improved sensitivity and selectivity.

Active Publication Date: 2013-06-12
常州庆源机械科技有限公司
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Problems solved by technology

Molecular imprinting is a molecular-specific recognition technology that has emerged in recent years. Combining molecular imprinting technology with electrochemical analysis technology can obtain high-sensitivity and high-selectivity biosensors. There have been related reports, but the application of trace gibberellin There is no relevant research on the detection of

Method used

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  • Method for utilizing molecularly imprinted electrochemical sensor to detect trace gibberellin A3
  • Method for utilizing molecularly imprinted electrochemical sensor to detect trace gibberellin A3
  • Method for utilizing molecularly imprinted electrochemical sensor to detect trace gibberellin A3

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Embodiment Construction

[0023] (1) Treatment of gold electrodes:

[0024] The gold electrodes were surface-polished with 1.0 μm, 0.3 μm and 0.05 μm alumina powder in turn, and then soaked and washed in 50% nitric acid and absolute ethanol for 5 min respectively, and were taken out and placed in pure water. Ultrasonic washing for 5 min, and then in 0.5 mol / L H 2 SO 4 The cyclic voltammetry (CV) electrochemical treatment was performed at -0.2-1.0 V until a stable cyclic voltammetry (CV) curve was obtained.

[0025] (2) Preparation of gibberellin A3 molecularly imprinted electrochemical sensor (prepared by electropolymerization of o-phenylenediamine):

[0026] Place the clean gold electrode treated in step (1) in a solution containing 1.5×10 -3 mol / L o-phenylenediamine and 5×10 -4 In a mixed solution of mol / L gibberellin A3, scan 30 cycles with cyclic voltammetry at a scan rate of 50 mV / s between 0 V and 0.8 V; take out the gold electrode and rinse it with double distilled water, then let it dry ...

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Abstract

The invention discloses a method for utilizing a molecularly imprinted electrochemical sensor to detect trace gibberellin A3. The method comprises the following steps of: o-phenylenediamine is taken as a function monomer, and gibberellin is adopted as a moldboard molecule to obtain a gibberellin A3 molecularly imprinted film through polymerization of mitallic electrode surface electricity; in the hydrogen peroxide-hydroquinone bottom solution with the pH of 7.4, the polyamide-amine arborization macromolecule complexed with a ferric ion can carry out catalyzing degradation on the hydrogen peroxide under energization; and the gibberellin A3 in the sample is utilized to replace functionalized arborization macromolecule gibberellin A3, so that the reduction strength of p-benzoquinone to hydroquinone is increased, and a method for detecting the gibberellin A3 is established. When the gibberellin A3 is in concentration range of 3*10-7mol / L-6*10-9mol / L, the lifting degree of an electrochemistry signal and the concentration of the gibberellin A3 is of a good linear relationship, and the method detection limit is 2.0*10-9 mol / L. The method provided by the invention improves the detective sensitivity and selectivity, and the detection of the light concentration gibberellin A3 is easy to be automated.

Description

technical field [0001] The invention relates to a method for rapidly measuring trace gibberellins by using molecular imprinting technology, functionalized dendritic macromolecular amplification effect and electrochemical sensor. Background technique [0002] Gibberellin (Gibberellin, GA) is a very important class of plant hormones and has been widely used in agricultural production. Gibberellin regulates various stages of plant growth and development, and has a significant effect on increasing the yield of vegetables and fruits. Different gibberellins have different biological activities, and gibberellin A3 (GA3) has the highest activity. However, studies have shown that gibberellin may accumulate in the human body after long-term use, causing chronic poisoning and even canceration, which poses a safety hazard to human health. At present, some developed countries have made strict regulations on the maximum residue limits of gibberellins in fruits, vegetables and beer. The...

Claims

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Application Information

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IPC IPC(8): G01N27/48
Inventor 李建平付丛魏小平
Owner 常州庆源机械科技有限公司
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