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Combined inactivate vaccine for haemophilus parasuis disease and streptococcus suis disease and preparation method for same

A technology of haemophilus porcine disease and double inactivated vaccine, which is applied in the direction of antibacterial drugs, pharmaceutical formulas, bacterial antigen components, etc., can solve the problem of inability to know whether the antigen inactivation is complete, large vaccine side effects, inflammation or swelling And other issues

Active Publication Date: 2013-06-19
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adjuvant of the vaccine is white oil, and the side effects of this adjuvant vaccine are relatively large, which will cause inflammation or swelling at the injection site; in addition, according to research data, the use of mineral oil adjuvants will stimulate and improve the quality of pigs such as pig rings to a certain extent. disease incidence
Moreover, in the preparation process of the vaccine, the obtained antigen has no inactivation effect judgment and related inspection, and it is impossible to know whether the antigen inactivation is complete, and the obtained antigen cannot be used as an antigen for vaccine production.

Method used

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  • Combined inactivate vaccine for haemophilus parasuis disease and streptococcus suis disease and preparation method for same
  • Combined inactivate vaccine for haemophilus parasuis disease and streptococcus suis disease and preparation method for same
  • Combined inactivate vaccine for haemophilus parasuis disease and streptococcus suis disease and preparation method for same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: Preparation of Haemophilus parasuis, Streptococcus suis dual inactivated vaccine (JS strain+ZJ strain+SC strain+Streptococcus equi zooepidemic subspecies ATCC35246 strain)

[0060] 1. Preparation of strains for production

[0061] Haemophilus parasuis serotype 4 JS strain, type 5 ZJ strain, Streptococcus suis type 2 SC strain, Streptococcus equi subsp. Serum type 4 JS strain, type 5 ZJ strain, Streptococcus suis type 2 SC strain, Streptococcus equi subspecies zooepidemic ATCC35246 strain for production.

[0062] (1) Primary seed propagation

[0063] First-class seed propagation was carried out on Haemophilus parasuis serotype 4 JS strain, type 5 ZJ strain, Streptococcus suis type 2 SC strain, and Streptococcus equi subspecies zooepidemic ATCC35246 strain. Streak and inoculate the freeze-dried strains of Haemophilus parasuis serotype 4 JS strain and type 5 ZJ strain on the TSA / NAD plate, culture at 37°C for 18 hours, select colonies that meet the requirem...

Embodiment 2

[0098] Embodiment 2: safety research

[0099] (1) Safety test of the vaccine on Balb / C mice

[0100] Three batches of prepared vaccines (batch numbers 09001, 09002, and 09003) were inoculated into 18-22 g Balb / C mice respectively, each subcutaneously injected with 0.4 ml, and observed for 14 days. The results are shown in Table 2. All Balb / C mice had no localized Responsive and all alive.

[0101] Table 2 vaccine is to the safety test result of Balb / C mouse

[0102] Vaccine batch Number of injections number of deaths local reactions only Pass rate 09001 10 0 0 5 / 5 09002 10 0 0 5 / 5 09003 10 0 0 5 / 5 control group 10 0 0 5 / 5

[0103] (2) Safety test of vaccine on piglets

[0104] Three batches of prepared vaccines (batch numbers 09001, 09002, and 09003) were injected intramuscularly into 5 30-day-old healthy susceptible pigs, each with 4 mL. There was no local reaction within 14 days a...

Embodiment 3

[0108] Embodiment 3: Efficacy test (tested in our company's experimental animal room)

[0109] (1) Vaccine efficacy test on Balb / C mice

[0110] Balb / C mice weighing 18 to 22 g were subcutaneously inoculated with 0.1 mL of dual inactivated vaccine, and divided into 3 groups 28 days after immunization, together with the control group of 10 mice in each group. Inject 5LD 50 (The bacterial content is 4.0×10 9 CFU), the type 5 ZJ strain was injected intraperitoneally with 5LD 50 (The bacterial content is 2.5×10 9 CFU); Streptococcus suis type 2 SC strain subcutaneous injection 2LD 50 (The bacterial content is 1.0×10 2 CFU), Streptococcus suis group C Streptococcus equi subspecies zooepidemic ATCC35246 subcutaneous injection 5LD 50 (The bacterial content is 4.0×10 2 CFU) another 10 were set as blank control, observed for 14 days, and the test results are shown in Table 4.

[0111] Table 4 vaccine is to Balb / C mouse effect test result

[0112]

[0113]

[0114] (2) Pi...

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Abstract

The invention relates to a combined inactivate vaccine for haemophilus parasuis disease and streptococcus suis disease and a preparation method for the same. The vaccine is prepared from inactivated haemophilus parasuis serotype-4 JS strain, haemophilus parasuis serotype-5 ZJ strain, streptococcus suis serotype-2 colony-R SC strain and streptococcus suis colony-C streptococcus equisubsp zooepidemius which are used as antigens, and adjuvants which are acceptable in veterinary medicine, and capable of performing immunoprophylaxis on the haemophilus parasuis disease caused by haemophilus parasuis, the streptococcus suis disease caused by streptococcus suis, and the mixed infection of haemophilus parasuis and streptococcus suis simultaneously. The vaccine is low in side effects, free from endotoxin and impure proteins in serum, good in immune safety, capable of achieving the effect of preventing multiple diseases by one injection in clinical and thus reducing cost, and capable of meeting the requirements of different users.

Description

technical field [0001] The invention relates to the technical field of animal biopharmaceuticals, in particular to a dual inactivated vaccine of Haemophilus parasuis and Streptococcus suis and a preparation method thereof. Background technique [0002] Haemophilus parasuis and Streptococcus suis are the two most important bacterial infectious diseases in pig herds in my country. Haemophilus parasuis disease is multiple serositis and arthritis of pigs caused by Haemophilus parasuis (Hps), which is also known as Glsser's Disease. Haemophilus parasuis can affect young pigs from 2 weeks to 4 months, mainly in post-weaning and nursery stages, usually seen in pigs aged 5 to 8 weeks, the incidence rate can reach 40%, and the mortality rate can reach 50% %. The main clinical symptoms were cough, dyspnea, emaciation, lameness, and rough coat; the main necropsy lesions were fibrinous pleurisy, pericarditis, peritonitis, arthritis, and meningitis. In addition, Haemophilus parasuis c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61P31/04A61K39/09A61K39/102
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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