Method for detecting infectious haematopoietic necrosis virus based on liquid-phase chip

A hematopoietic organ necrosis and infectivity technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Polluted environment, short time required, good specific effect
CN103173569AInactive Publication Date: 2013-06-26INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2013-06-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting an infectious haematopoietic necrosis virus based on liquid-phase chip. The invention provides a specific primer pair for identifying the infectious haematopoietic necrosis virus in an auxiliary manner, wherein the specific primer pair is composed of a single-chain DNA (Deoxyribonucleic Acid) molecule as shown in sequence 1 of a sequence table and the single-chain DNA molecule as shown in sequence 2 of the sequence table. The invention further protects a primer probe composition for identifying the infectious haematopoietic necrosis virus in the auxiliary manner, wherein the primer probe composition is composed of the specific primer pair and a probe T1; and a nucleotide sequence of the probe T1 is as shown in sequence 3 of the sequence table. The specific primer pair provided by the invention has good specificity in identifying the infectious haematopoietic necrosis virus. The primer probe composition provided by the invention is combined with the liquid-phase chip to identify the infectious haematopoietic necrosis virus, and therefore, the method has the advantages of being good in specificity, high in sensitivity, simple to operate, short in needed time, free of environmental pollution, free of health risks to the people and capable of carrying out high-flux detection.
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Description

technical field

[0001] The invention relates to a primer pair, a primer probe composition and their application for assisting in the identification of infectious hematopoietic organ necrosis virus, in particular to a method for detecting infectious hematopoietic organ necrosis virus based on a liquid phase chip. Background technique

[0002] Infectious hematopoietic necrosis (IHN) is a second-class infectious disease listed in the "List of Class I and Class II Infectious Diseases and Parasitic Diseases of Imported Animals of the People's Republic of China" It is an important epidemic disease in the OIE list of the International Office of Epizootics.

[0003] Fish infectious hematopoietic necrosis is caused by infectious hematopoietic necrosis virus (IHNV). IHNV is an RNA virus, a member of the Rhabdoviridae family, approximately 120-180 nm long. IHNV can infect a variety of seawater and freshwater cultured fish, and is prevalent in Europe, the United States, Japan, Korea a...

Claims

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